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Home / Equine Research Bank / Can J Vet Res / Circadian rhythms of osteocalcin in equine serum. Correlatio...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1991; 55(1); 5-10;

Circadian rhythms of osteocalcin in equine serum. Correlation with alkaline phosphatase, calcium, phosphate and total protein levels.

Abstract: The purpose of the study was to determine whether there were circadian variations in serum osteocalcin in normal horses and to determine whether it was important to regulate the time of blood sampling in clinical investigations. Osteocalcin or bone Gla-protein (BGP), alkaline phosphatase, total calcium, phosphate and total protein were studied over a 24 h period. Blood samples were taken every 60 min from nine adult Standardbred horses. There was a correlation between serum levels of alkaline phosphatase (r = 0.3, p less than 0.01), phosphate (r = 0.42, p less than 0.01) and serum osteocalcin levels. There was a very marked individual effect on serum levels of osteocalcin and alkaline phosphatase (p less than 0.01). This effect was present for phosphate levels but not significant for total calcium. The individual effect was lower and time effect was higher for serum osteocalcin if the subjects were divided into two age groups, one of horses of five years or less (n = 4) and a second group older than five years (n = 5). In both groups a circadian rhythmicity was observed. Serum osteocalcin showed a biphasic pattern. Levels were constant during daytime (light period) and underwent significant variations during the night (dark period), going through a nadir at 2000 h and through a maximum peak at 0500 h. It was concluded that in normal horses the blood osteocalcin level follows a circadian variation. Also daytime (light period) seems to be the more appropriate period for blood sampling.
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Publication Date: 1991-01-01 PubMed ID: 1884284PubMed Central: PMC1263405
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the circadian variations in serum osteocalcin levels in healthy horses and investigates its relationship with alkaline phosphatase, calcium, phosphate, and total protein levels. By doing so, it presents crucial insights that can be used to determine the ideal time for blood sampling in clinical examinations.

Objective and Methodology

  • The primary objective of this study was to investigate if there were circadian variations in serum osteocalcin levels in healthy horses. Researchers also aimed to figure out if these variations necessitated time-specific blood sampling for precise clinical investigation.
  • To achieve this, nine adult Standardbred horses were studied over a 24-hour duration. Blood samples were taken every 60 minutes.
  • The researchers studied the serum levels of osteocalcin (also known as bone Gla-protein or BGP), alongside alkaline phosphatase, total calcium, phosphate, and total protein in each of the collected samples.

Results

  • Results revealed that serum osteocalcin levels correlated with alkaline phosphatase and phosphate levels.
  • A notably individual effect on serum levels of osteocalcin and alkaline phosphatase was found, which was also present for phosphate levels but not for total calcium.
  • When the horses were divided into two age groups—those five years or less and those older than five years—it was found that the individual effect decreased for osteocalcin, but the time effect was more prominent.
  • Circadian rhythmicity was observed for osteocalcin serum levels in both age groups, exhibiting a biphasic pattern. The levels were stable during daylight hours, but showed significant fluctuations during nighttime, reaching the lowest levels at 8:00 PM and peaking at 5:00 AM.

Conclusion

  • The study concluded that osteocalcin levels in horse blood display a circadian rhythm. As such, sampling blood during daylight hours would be more appropriate for clinical studies, given the stability of osteocalcin levels throughout this period.

Cite This Article

APA
Lepage OM, DesCôteaux L, Marcoux M, Tremblay A. (1991). Circadian rhythms of osteocalcin in equine serum. Correlation with alkaline phosphatase, calcium, phosphate and total protein levels. Can J Vet Res, 55(1), 5-10.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 55
Issue: 1
Pages: 5-10

Researcher Affiliations

Lepage, O M
  • Département de Médecine, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
DesCôteaux, L
    Marcoux, M
      Tremblay, A

        MeSH Terms

        • Alkaline Phosphatase / blood
        • Animals
        • Blood Proteins / analysis
        • Calcium / blood
        • Circadian Rhythm
        • Female
        • Horses / blood
        • Male
        • Osteocalcin / blood
        • Phosphates / blood
        • Pregnancy
        • Pregnancy, Animal / blood
        • Reference Values

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        Citations

        This article has been cited 3 times.
        1. Muñoz-Prieto A, Cerón JJ, Rubio CP, Contreras-Aguilar MD, Pardo-Marín L, Ayala-de la Peña I, Martín-Cuervo M, Holm Henriksen IM, Arense-Gonzalo JJ, Tecles F, Hansen S. Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome. Animals (Basel) 2022 Nov 23;12(23).
          doi: 10.3390/ani12233261pubmed: 36496782google scholar: lookup
        2. Lepage OM, Marcoux M. Comparison of serum osteocalcin concentration and alkaline phosphatase activity as indicators of bone activity in a foal following surgery. Can Vet J 1991 Apr;32(4):239-40.
          pubmed: 17423770
        3. Lepage OM, Marcoux M, Tremblay A, Dumas G. Sex does not influence serum osteocalcin levels in standardbred horses of different ages. Can J Vet Res 1992 Oct;56(4):379-81.
          pubmed: 1477808
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