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Home / Equine Research Bank / Sci Rep / Circulating miR-146b and miR-27b are efficient biomarkers fo...
Scientific reports2023; 13(1); 7966; doi: 10.1038/s41598-023-35207-3

Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis.

Abstract: One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA.
© 2023. The Author(s).
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Publication Date: 2023-05-17 PubMed ID: 37198318PubMed Central: PMC10192321DOI: 10.1038/s41598-023-35207-3Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigated potentially effective biomarkers for the early diagnosis of osteoarthritis in donkeys. The specific biomarkers explored were miR-146b and miR-27b, and the research indicated these could be potential tools for early detection of this common equine ailment.

Introduction and Methodology

  • In this study, the researchers aimed to identify potential early biomarkers for the detection of osteoarthritis (OA), a prevalent orthopedic issue in the Equidae family, which includes horses, donkeys, and zebras.
  • OA was induced in nine donkeys using a single intra-articular injection of 25 mg of monoiodoacetate (MIA) into the left radiocarpal joint. MIA is a known OA-inducing agent, catalyzing the degeneration of cartilage in a manner similar to the degenerative disease.
  • Throughout different stages of the administered OA, the researchers monitored specific biochemical, epigenetic, and transcriptomic factors, sampling serum and synovial fluid at various intervals.

Assessment and Findings

  • During the trial, the researchers observed and accounted for levels of total GAGs (Glycosaminoglycans) and CS (Chondroitin Sulfate), as well as the gene expression of miR-146b, miR-27b, TRAF-6, and COL10A1.
  • Data showed that total GAGs and CS levels increased at different stages of OA, suggesting they might be indicative biomarkers for OA detection.
  • Focusing on transcriptomics, the researchers noticed variations in the expression of miR-146b, miR-27b, TRAF-6, and COL10A1 genes over different stages of OA progression.
  • For instance, the level of miR-146b and miR-27b expression was upregulated as OA progressed, reducing at the late stages. The TRAF-6 gene also saw increased activity at the late stages of the disease.
  • Interestingly, the COL10A1 gene appeared to be overexpressed in the early stages, then decreased at the later stages.

Conclusions and Implications

  • From these findings, the researchers concluded that both miR-146b and miR-27b, along with COL10A1, could be effective noninvasive early biomarkers for OA diagnosis.
  • Being able to identify osteoarthritis in its early stages through the use of these biomarkers could pave the way for earlier and more effective treatments, potentially slowing the progression of this degenerative condition.

Cite This Article

APA
Yassin AM, AbuBakr HO, Abdelgalil AI, Farid OA, El-Behairy AM, Gouda EM. (2023). Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis. Sci Rep, 13(1), 7966. https://doi.org/10.1038/s41598-023-35207-3

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 7966
PII: 7966

Researcher Affiliations

Yassin, Aya M
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. ayamohye@cu.edu.eg.
AbuBakr, Huda O
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Abdelgalil, Ahmed I
  • Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Farid, Omar A
  • Department of Physiology, National Organization for Drug Control and Research, Giza, Egypt.
El-Behairy, Adel M
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Gouda, Eman M
  • Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Early Diagnosis
  • Equidae / genetics
  • MicroRNAs / metabolism
  • Osteoarthritis / diagnosis
  • Osteoarthritis / genetics
  • Osteoarthritis / metabolism

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 1 times.
  1. Felekkis K, Pieri M, Papaneophytou C. Exploring the Feasibility of Circulating miRNAs as Diagnostic and Prognostic Biomarkers in Osteoarthritis: Challenges and Opportunities.. Int J Mol Sci 2023 Aug 24;24(17).
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