Cladophora glomerata methanolic extract decreases oxidative stress and improves viability and mitochondrial potential in equine adipose derived mesenchymal stem cells (ASCs).
Abstract: Reactive oxygen species (ROS) are key mediators of several cellular damage and thus associated with equine diseases such as inflammation and metabolic syndrome. This study aimed to evaluate the protective and antioxidant activities of methanolic extract prepared from Cladophora glomerata (C. glomerata) biomass, on equine adipose derived mesenchymal stem cells (EqASCs), under experimental oxidative stress induced by HO. Pre-treatment of EqASCs cells with different concentrations of C. glomerata methanolic extract (1% and 5%) provided a clear protection against cellular damage triggered by HO. The cell's apoptotic status was significantly regulated, with promotion of cell viability, down-regulation of pro-apoptotic (p21, p53, Bax and Casp-9) genes expression, concomitant to up-regulation of the survival gene Bcl-2, this being supported by a mitigation of the endoplasmic reticulum (ER) stress and significant minimization of mitochondrial dysfunction. The results also showed that C. glomerata extract significantly increased the antioxidant enzymes Superoxide dismutase (SOD) and Catalase (CAT) activities, positively regulated the enzymes genes expression, and markedly reduced the protein carbonyls derivatives production. Finally, RT-qPCR analysis of the inflammatory related genes allowed to highlight a promising anti-inflammatory and immunomodulatory effect of this extract. Due to the valuable antioxidant and anti-inflammatory activities, C. glomerata may have potential benefits for the prevention of equine diseases associated with oxidative stress, including metabolic syndrome.
Copyright © 2018 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
Publication Date: 2018-12-12 PubMed ID: 30553132DOI: 10.1016/j.biopha.2018.12.020Google Scholar: Lookup
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- Journal Article
Summary
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The research article focuses on the potential protective and antioxidant properties of the methanolic extract of Cladophora glomerata, a type of algae, on horse adipose-derived mesenchymal stem cells (ASCs) under oxidativestress.
Objective
- The main aim of the study was to test the protective qualities and the antioxidant activities of methanolic extract derived from Cladophora glomerata algae on equine adipose stem cells (EqASCs). The study puts the EqASCs under artificially induced oxidative stress to observe the action of the extract.
Methods and Results
- Experimental oxidative stress was induced in EqASCs using hydrogen peroxide (HO). Then, the cells were pre-treated with different concentrations of C. glomerata methanolic extract (1% and 5%).
- The results indicated that the extract effectively protected the cells from damage instigated by oxidative stress. It was observed that the apoptotic (cell death) status of the EqASCs was considerably regulated by.
- In essence, the study observed an increase in cell viability, down-regulation of pro-apoptotic genes’ expression (p21, p53, Bax and Casp-9), and up-regulation of the survival gene (Bcl-2).
- Furthermore, the extract helped mitigate the stress in the endoplasmic reticulum and significantly minimize mitochondrial dysfunction.
Antioxidant Enzymes
- The study also revealed that C. glomerata extract significantly boosted the activities of antioxidant enzymes Superoxide dismutase (SOD) and Catalase (CAT).
- In addition, it positively regulated the expressions of the enzymes’ genes and significantly reduced the production of protein carbonyls derivatives.
Anti-Inflammatory and Immunomodulatory Effects
- An RT-qPCR analysis was performed on inflammatory-related genes which unveiled a promising anti-inflammatory and immunomodulatory effect of the C. glomerata extract.
- This suggests that, due to its potent antioxidant and anti-inflammatory properties, C. glomerata may hold potential benefits in preventing equine diseases linked with oxidative stress. This includes conditions such as metabolic syndrome.
Cite This Article
APA
Bourebaba L, Michalak I, Röcken M, Marycz K.
(2018).
Cladophora glomerata methanolic extract decreases oxidative stress and improves viability and mitochondrial potential in equine adipose derived mesenchymal stem cells (ASCs).
Biomed Pharmacother, 111, 6-18.
https://doi.org/10.1016/j.biopha.2018.12.020 Publication
Researcher Affiliations
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Norwida 27B, 50-375 Wrocław, Poland.
- Department of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, Smoluchowskiego 25, 50-372 Wrocław, Poland.
- Faculty of Veterinary Medicine, Equine Clinic - Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany.
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Norwida 27B, 50-375 Wrocław, Poland; Faculty of Veterinary Medicine, Equine Clinic - Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany. Electronic address: krzysztof.marycz@upwr.edu.pl.
MeSH Terms
- Adipose Tissue / drug effects
- Adipose Tissue / metabolism
- Animals
- Cell Survival / drug effects
- Cell Survival / physiology
- Cells, Cultured
- Dose-Response Relationship, Drug
- Horses
- Membrane Potential, Mitochondrial / drug effects
- Membrane Potential, Mitochondrial / physiology
- Mesenchymal Stem Cells / drug effects
- Mesenchymal Stem Cells / metabolism
- Oxidative Stress / drug effects
- Oxidative Stress / physiology
- Plant Extracts / isolation & purification
- Plant Extracts / pharmacology
- Seaweed / isolation & purification
- Seaweed / metabolism
Citations
This article has been cited 10 times.- Dziergowska K, Wełna M, Szymczycha-Madeja A, Chęcmanowski J, Michalak I. Valorization of Cladophora glomerata Biomass and Obtained Bioproducts into Biostimulants of Plant Growth and as Sorbents (Biosorbents) of Metal Ions. Molecules 2021 Nov 16;26(22).
- Li SW, Cai Y, Mao XL, He SQ, Chen YH, Yan LL, Zhou JJ, Song YQ, Ye LP, Zhou XB. The Immunomodulatory Properties of Mesenchymal Stem Cells Play a Critical Role in Inducing Immune Tolerance after Liver Transplantation. Stem Cells Int 2021;2021:6930263.
- Aldbass A, Amina M, Al Musayeib NM, Bhat RS, Al-Rashed S, Marraiki N, Fahmy R, El-Ansary A. Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays. Sci Rep 2021 Apr 19;11(1):8512.
- Bourebaba L, Kornicka-Garbowska K, Al Naem M, Röcken M, Łyczko J, Marycz K. MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress. Stem Cell Res Ther 2021 Feb 3;12(1):97.
- Bourebaba L, Michalak I, Baouche M, Kucharczyk K, Fal AM, Marycz K. Cladophora glomerata enriched by biosorption with Mn(II) ions alleviates lipopolysaccharide-induced osteomyelitis-like model in MC3T3-E1, and 4B12 osteoclastogenesis. J Cell Mol Med 2020 Jul;24(13):7282-7300.
- Bourebaba L, Michalak I, Baouche M, Kucharczyk K, Marycz K. Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome. Stem Cell Res Ther 2019 Dec 17;10(1):392.
- Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
- You Y, Wen DG, Gong JP, Liu ZJ. Research Status of Mesenchymal Stem Cells in Liver Transplantation. Cell Transplant 2019 Dec;28(12):1490-1506.
- Kot M, Bronowicka-Adamska P, Tyszka-Czochara M. Regulation of Human Stem Cells by Functional Food Components: How Vitamins, Minerals and Phytochemicals Influence Mesenchymal Stem Cells' Fate and Function. Nutrients 2025 Nov 13;17(22).
- Almalki SG, Al-Ghamdi YO, Algethami FK, Daoush WM, Alrfaei BM, Alanazi M, Alqurashi YE, Jabli M. Critical Evaluation of Combined Cu and Fe(3)O(4) Nanodots with Gum Arabic Nano-Hybrids Using in-vitro Model: Flow Cytometry and Cell Viability Studies. Int J Nanomedicine 2025;20:2593-2607.
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