FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
The veterinary quarterly2014; 34(2); 92-97; doi: 10.1080/01652176.2014.949390

Clinical follow-up of horses treated with allogeneic equine mesenchymal stem cells derived from umbilical cord blood for different tendon and ligament disorders.

Abstract: Mesenchymal stem cells (MSCs) offer promise as therapeutic aids in the repair of tendon and ligament disorders in sport horses. Equine allogeneic MSCs derived from umbilical cord blood (eUCB-MSCs) can be obtained in a minimally invasive fashion with successful propagation of MSCs. Objective: The objective of this study was to determine the applicability and therapeutic effect of eUCB-MSCs on tendinitis of the superficial digital flexor tendon, desmitis of the suspensory ligament, tendinitis of the deep digital flexor tendon, and desmitis of the inferior check ligament in clinical cases. Methods: A retrospective clinical study was performed. At two equine clinics, 52 warmblood horses were treated with cultured eUCB-MSCs between 2009 and 2012. About 2-10 × 10(6) cells per lesion were administered. When a lesion was treated twice, the total amount could run up to 20 × 10(6) cells. Pearson's chi-squared test was used to compare the effect of the injured structure on the success rate, as well as the effect of the age of the horse. Results: Based on repeated examinations, 40 horses (77%) returned to work on the same or a higher level based on information provided by the owner. Neither the injured structure nor the age of the horse had a statistically significant influence on the result. Conclusions: Overall, the results of treatment of some tendon and ligament injuries with eUCB-MSCs in clinical cases are promising.
Get Full Text
Publication Date: 2014-08-26 PubMed ID: 25072527DOI: 10.1080/01652176.2014.949390Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper assesses the therapeutic effects of using Mesenchymal stem cells (MSCs) drawn from equine-derived Umbilical Cord Blood, known as eUCB-MSCs, in treating tendon and ligament disorders in sport horses. The findings reveal that 77% of the treated horses were able to return to work at their previous or better capacity, suggesting promising results.

Research Objective

The main aim of this research was to investigate the effectiveness of eUCB-MSCs in treating four specific disorders: tendinitis of the superficial digital flexor tendon, desmitis of the suspensory ligament, tendinitis of the deep digital flexor tendon, and desmitis of the inferior check ligament. This was done through a retrospective clinical study of sport horses treated with this therapy between 2009 and 2012.

Methodology

  • A retrospective clinical study was conducted using data from two equine clinics.
  • The study observed a total of 52 warmblood horses that received treatment with eUCB-MSCs between 2009 and 2012.
  • Each lesion was treated with approximately 2-10×10(6) cells. In some cases, where the lesion was treated twice, the overall amount of cells could reach up to 20×10(6).
  • The research applied Pearson’s chi-squared test to evaluate the effect of the injured structure on the success rate, and to determine the impact of the horse’s age on the results.

Findings

  • Out of the 52 warmblood horses treated, 40 (77%) were able to return to work at the same or higher level of activity as per the data provided by the owners.
  • The researchers found no statistically significant correlation between the age of the horse or the type of injury and the success of the treatment.

Conclusions

  • The use of eUCB-MSCs to treat tendon and ligament disorders in sport horses shows promise, based on this study’s findings. The high success rate of horses returning to their previous performance level suggests the treatment is effective.
  • Neither the age of the horse nor the type of injured structure posed a significant influence on the results, suggesting the treatment could be broadly applicable.

Cite This Article

APA
(2014). Clinical follow-up of horses treated with allogeneic equine mesenchymal stem cells derived from umbilical cord blood for different tendon and ligament disorders. Vet Q, 34(2), 92-97. https://doi.org/10.1080/01652176.2014.949390

Publication

The veterinary quarterly
ISSN: 1875-5941
NlmUniqueID: 7909485
Country: England
Language: English
Volume: 34
Issue: 2
Pages: 92-97

Researcher Affiliations

MeSH Terms

  • Animals
  • Cord Blood Stem Cell Transplantation / veterinary
  • Female
  • Horses / injuries
  • Lameness, Animal / diagnostic imaging
  • Lameness, Animal / therapy
  • Ligaments / injuries
  • Male
  • Mesenchymal Stem Cell Transplantation / veterinary
  • Mesenchymal Stem Cells
  • Retrospective Studies
  • Sports
  • Tendinopathy / diagnostic imaging
  • Tendinopathy / therapy
  • Tendinopathy / veterinary
  • Transplantation, Homologous / veterinary
  • Ultrasonography

Citations

This article has been cited 29 times.
  1. Stage HJ, Trappe S, Söllig K, Trachsel DS, Kirsch K, Zieger C, Merle R, Aschenbach JR, Gehlen H. Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources. Animals (Basel) 2023 Apr 15;13(8).
    doi: 10.3390/ani13081352pubmed: 37106915google scholar: lookup
  2. El-Husseiny HM, Mady EA, Helal MAY, Tanaka R. The Pivotal Role of Stem Cells in Veterinary Regenerative Medicine and Tissue Engineering. Vet Sci 2022 Nov 21;9(11).
    doi: 10.3390/vetsci9110648pubmed: 36423096google scholar: lookup
  3. Duddy HR, Schoonover MJ, Hague BA. Outcome following local injection of a liquid amnion allograft for treatment of equine tendonitis or desmitis - 100 cases. BMC Vet Res 2022 Nov 7;18(1):391.
    doi: 10.1186/s12917-022-03480-5pubmed: 36345002google scholar: lookup
  4. Kearney CM, Khatab S, van Buul GM, Plomp SGM, Korthagen NM, Labberté MC, Goodrich LR, Kisiday JD, Van Weeren PR, van Osch GJVM, Brama PAJ. Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation. Front Vet Sci 2022;9:907616.
    doi: 10.3389/fvets.2022.907616pubmed: 35812845google scholar: lookup
  5. Soukup R, Gerner I, Gültekin S, Baik H, Oesterreicher J, Grillari J, Jenner F. Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells. Int J Mol Sci 2022 May 23;23(10).
    doi: 10.3390/ijms23105858pubmed: 35628667google scholar: lookup
  6. Depuydt E, Broeckx SY, Chiers K, Patruno M, Da Dalt L, Duchateau L, Saunders J, Pille F, Martens A, Van Hecke L, Spaas JH. Cellular and Humoral Immunogenicity Investigation of Single and Repeated Allogeneic Tenogenic Primed Mesenchymal Stem Cell Treatments in Horses Suffering From Tendon Injuries. Front Vet Sci 2021;8:789293.
    doi: 10.3389/fvets.2021.789293pubmed: 35281431google scholar: lookup
  7. Meeremans M, Van de Walle GR, Van Vlierberghe S, De Schauwer C. The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research. Front Cell Dev Biol 2021;9:651164.
    doi: 10.3389/fcell.2021.651164pubmed: 34012963google scholar: lookup
  8. Kamm JL, Riley CB, Parlane N, Gee EK, McIlwraith CW. Interactions Between Allogeneic Mesenchymal Stromal Cells and the Recipient Immune System: A Comparative Review With Relevance to Equine Outcomes. Front Vet Sci 2020;7:617647.
    doi: 10.3389/fvets.2020.617647pubmed: 33521090google scholar: lookup
  9. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
    doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
  10. Chung MJ, Son JY, Park S, Park SS, Hur K, Lee SH, Lee EJ, Park JK, Hong IH, Kim TH, Jeong KS. Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases. Int J Stem Cells 2021 May 30;14(2):150-167.
    doi: 10.15283/ijsc20167pubmed: 33377459google scholar: lookup
  11. Zygmuntowicz A, Burmańczuk A, Markiewicz W. Selected Biological Medicinal Products and Their Veterinary Use. Animals (Basel) 2020 Dec 9;10(12).
    doi: 10.3390/ani10122343pubmed: 33316993google scholar: lookup
  12. Bukowska J, Szóstek-Mioduchowska AZ, Kopcewicz M, Walendzik K, Machcińska S, Gawrońska-Kozak B. Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science. Stem Cell Rev Rep 2021 Jun;17(3):719-738.
    doi: 10.1007/s12015-020-10049-ypubmed: 33025392google scholar: lookup
  13. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
    doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
  14. Voga M, Adamic N, Vengust M, Majdic G. Stem Cells in Veterinary Medicine-Current State and Treatment Options. Front Vet Sci 2020;7:278.
    doi: 10.3389/fvets.2020.00278pubmed: 32656249google scholar: lookup
  15. Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1β model of synovitis. Equine Vet J 2020 Jul;52(4):601-612.
    doi: 10.1111/evj.13222pubmed: 31821594google scholar: lookup
  16. Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges. Stem Cell Res Ther 2019 Jun 18;10(1):181.
    doi: 10.1186/s13287-019-1291-0pubmed: 31215490google scholar: lookup
  17. Liu L, Hindieh J, Leong DJ, Sun HB. Advances of stem cell based-therapeutic approaches for tendon repair. J Orthop Translat 2017 Apr;9:69-75.
    doi: 10.1016/j.jot.2017.03.007pubmed: 29662801google scholar: lookup
  18. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
    doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
  19. Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses. Stem Cells Transl Med 2018 Jan;7(1):98-108.
    doi: 10.1002/sctm.17-0071pubmed: 29063737google scholar: lookup
  20. Beerts C, Suls M, Broeckx SY, Seys B, Vandenberghe A, Declercq J, Duchateau L, Vidal MA, Spaas JH. Tenogenically Induced Allogeneic Peripheral Blood Mesenchymal Stem Cells in Allogeneic Platelet-Rich Plasma: 2-Year Follow-up after Tendon or Ligament Treatment in Horses. Front Vet Sci 2017;4:158.
    doi: 10.3389/fvets.2017.00158pubmed: 29018808google scholar: lookup
  21. Vandenberghe A, Broeckx SY, Beerts C, Seys B, Zimmerman M, Verweire I, Suls M, Spaas JH. Tenogenically Induced Allogeneic Mesenchymal Stem Cells for the Treatment of Proximal Suspensory Ligament Desmitis in a Horse. Front Vet Sci 2015;2:49.
    doi: 10.3389/fvets.2015.00049pubmed: 26664976google scholar: lookup
  22. Dai L, Hu X, Zhang X, Zhu J, Zhang J, Fu X, Duan X, Ao Y, Zhou C. Different tenogenic differentiation capacities of different mesenchymal stem cells in the presence of BMP-12. J Transl Med 2015 Jun 24;13:200.
    doi: 10.1186/s12967-015-0560-7pubmed: 26104414google scholar: lookup
  23. Cao M, Tong M, Pu Y, Lv X, Sun Y, Zhang H, Sheng R, Wang H, Gao Y, Dai G, Xiong F, Wang Q, Rui Y. Human Umbilical Cord-Derived Mesenchymal Stem Cells-Involved Strategies: Advancing Tendon Injuries Therapy Towards Clinical Translation. Stem Cell Rev Rep 2026 Apr;22(3):1177-1199.
    doi: 10.1007/s12015-025-11054-9pubmed: 41528668google scholar: lookup
  24. Barrachina L, Ivanovska A, Eslami Arshaghi T, O'Brien A, Cequier A, Murphy M, Hollinshead F, Rodellar C, Barry F. Generation of equine induced pluripotent stem cells from cells of embryonic, perinatal and adult tissues. Stem Cell Res Ther 2025 Oct 8;16(1):547.
    doi: 10.1186/s13287-025-04671-1pubmed: 41063189google scholar: lookup
  25. Guest DJ, Birch HL, Thorpe CT. A review of the equine suspensory ligament: Injury prone yet understudied. Equine Vet J 2025 Sep;57(5):1167-1182.
    doi: 10.1111/evj.14447pubmed: 39604165google scholar: lookup
  26. Giraldo A, Koch TG, Madan P, Lepage S, Monteith G, Alizadeh AH, Tran A, Mortagy N, Koenig JB. Effect of extracorporeal shockwave therapy on the immunomodulatory and anti-inflammatory properties of cultured equine umbilical cord blood mesenchymal stromal cells. Can J Vet Res 2024 Jul;88(3):87-93.
    pubmed: 38988333
  27. Liang W, Zhou C, Deng Y, Fu L, Zhao J, Long H, Ming W, Shang J, Zeng B. The current status of various preclinical therapeutic approaches for tendon repair. Ann Med 2024 Dec;56(1):2337871.
    doi: 10.1080/07853890.2024.2337871pubmed: 38738394google scholar: lookup
  28. Taguchi T, Lopez M, Takawira C. Viable tendon neotissue from adult adipose-derived multipotent stromal cells. Front Bioeng Biotechnol 2023;11:1290693.
    doi: 10.3389/fbioe.2023.1290693pubmed: 38260742google scholar: lookup
  29. Tamura N, Heidari N, Faragher RGA, Smith RKW, Dudhia J. Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. J Equine Sci 2023 Sep;34(3):67-72.
    doi: 10.1294/jes.34.67pubmed: 37781569google scholar: lookup
Subscribe to our newsletter
Join 99,850 horse owners like you who receive our email updates on horse health and nutrition.