Clinical Observations After Prebreeding Intrauterine Plasma Infusion in 18 Mares Inseminated With Thawed Frozen Semen.
Abstract: The use of autologous blood processing tools including platelet-rich plasma (PRP) devices is increasingly widespread in veterinary medicine. In equine reproduction, a number of studies have explored the effects of intrauterine infusion of PRP on persistent mating-induced endometritis. Artificial insemination with thawed frozen semen incites an intrauterine inflammatory response and we sought to extend the applications of intrauterine PRP to normal mares being inseminated with frozen semen. We investigated a subset of our normal breeding population to observe the clinical effects of prebreeding intrauterine infusion of PRP and plasma followed by insemination with frozen semen. Eighteen mares were treated after failure to achieve pregnancy after one breeding cycle of artificial insemination with frozen semen. Breeding management was performed in a routine manner, and each mare was bred to the same stallion as the previous cycle. Autologous PRP was prepared using a commercial platelet isolation device; PRP was diluted with Platelet-poor Plasma, and intrauterine infusion was performed 12-48 hours before artificial insemination. This case series represents an initial investigation into the clinical effects of intrauterine PRP and plasma infused before insemination with thawed frozen semen in a population of clinically normal mares.
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication Date: 2021-01-16 PubMed ID: 33781416DOI: 10.1016/j.jevs.2021.103389Google Scholar: Lookup
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Summary
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The research article explores the effects of using platelet-rich plasma (PRP) within the uterus before inseminating horses with previously frozen semen, in order to increase pregnancy rates.
Context of the Study
- The study is situated in the field of veterinary medicine, particularly dealing with equine reproduction.
- In recent years, the use of autologous blood processing tools including Platelet-Rich Plasma (PRP) devices has been increasingly adopted in veterinary medicine.
Purpose of the Study and Methodology
- The main objective of the study was to investigate the clinical effects of prebreeding intrauterine infusion of PRP and Plasma, followed by insemination with thawed frozen semen.
- Artificial insemination with thawed frozen semen causes an intrauterine inflammatory response, hence the researchers explored the applications of intrauterine PRP to normal mares being inseminated with frozen semen.
- They carried out their investigation on a subset of the normal breeding population.
- The PRP was obtained from the mares using a commercial platelet isolation device, it was then diluted with Platelet-poor Plasma, and intrauterine infusion was carried out 12-48 hours before artificial insemination.
Participants in the Study
- A total of 18 mares were included in the study with each one being treated when they did not get pregnant after one breeding cycle of artificial insemination with frozen semen.
- For all the mares, the breeding management was handled in a routine manner, and each was bred to the same stallion as in the previous cycle.
Significance of the Study and its Future Implications
- This research represents a significant initial move towards understanding the clinical effects associated with infusing intrauterine PRP and plasma before insemination with thawed frozen semen, particularly in a population of clinically normal mares.
- The findings of the paper could have implications in optimizing procedures for artificial insemination in horses and potentially in other livestock.
Cite This Article
APA
Pasch L, Schmidt A, King W.
(2021).
Clinical Observations After Prebreeding Intrauterine Plasma Infusion in 18 Mares Inseminated With Thawed Frozen Semen.
J Equine Vet Sci, 99, 103389.
https://doi.org/10.1016/j.jevs.2021.103389 Publication
Researcher Affiliations
- Rhinebeck Equine LLP, Rhinebeck, NY. Electronic address: lpasch@rhinebeckequine.com.
- Wisconsin Equine Clinic and Hospital, Oconomowoc, WI.
- Owl Manor Medical, Warsaw, IN.
MeSH Terms
- Animals
- Endometritis / veterinary
- Female
- Horse Diseases
- Horses
- Insemination, Artificial / veterinary
- Male
- Platelet-Rich Plasma
- Pregnancy
- Reproduction
- Semen Preservation / veterinary
Citations
This article has been cited 2 times.- Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
- Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel) 2021 Apr 23;10(5).
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