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Home / Equine Research Bank / Infect Immun / Cloning and molecular characterization of an immunogenic Lig...
Infection and immunity2002; 70(11); 5924-5930; doi: 10.1128/IAI.70.11.5924-5930.2002

Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans.

Abstract: A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that had recently aborted due to leptospiral infection. LigA is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. A search of the NCBI database found that homology of the LigA repeat region was limited to an immunoglobulin-like domain of the bacterial intimin binding protein of Escherichia coli, the cell adhesion domain of Clostridium acetobutylicum, and the invasin of Yersinia pestis. Secondary structure prediction analysis indicates that LigA consists mostly of beta sheets with a few alpha-helical regions. No LigA was detectable by immunoblot analysis of lysates of the leptospires grown in vitro at 30 degrees C or when cultures were shifted to 37 degrees C. Strikingly, immunohistochemistry on kidney from leptospira-infected hamsters demonstrated LigA expression. These findings suggest that LigA is specifically induced only in vivo. Sera from horses, which aborted as a result of natural Leptospira infection, strongly recognize LigA. LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection. Thus, LigA may have value in serodiagnosis or as a protective immunogen in novel vaccines.
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Publication Date: 2002-10-16 PubMed ID: 12379666PubMed Central: PMC130282DOI: 10.1128/IAI.70.11.5924-5930.2002Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focused on the discovery and analysis of an immunoreactive protein, known as LigA, from the bacteria Leptospira interrogans. It highlighted that this protein, which appears to be uniquely expressed during infection, might prove useful in the development of new diagnostic methods or vaccines for leptospirosis.

Introduction

  • The paper discusses research conducted around a newly identified protein from the bacterium Leptospira interrogans, known as LigA. This bacterium is linked to leptospirosis, a disease that can lead to kidney damage, meningitis, liver failure, and respiratory distress.
  • The existence of LigA was discovered while examining genomic DNA of the bacteria using serum from a mare that had suffered a recent abortion due to a leptospira infection.

Characterization of LigA

  • LigA is a large protein of 130 kilodaltons, encoded by an open reading frame of 3,675 base pairs. The protein is built up from multiple repetitions of a 90 amino acid sequence.
  • Searches of the NCBI database revealed some similarity between LigA and proteins found in other bacterial species such as Escherichia coli, Clostridium acetobutylicum, and Yersinia pestis.
  • Structural analysis suggests that LigA consists mostly of beta-sheet structures with a few alpha-helical regions.

Expression of LigA

  • No evidence of LigA expression was found in leptospira cultures grown in the lab at either 30 degrees Celsius or after temperature was increased to 37 degrees Celsius, mimicking conditions inside a mammalian host.
  • However, LigA expression was detected when examining kidney tissue from hamsters infected with leptospira bacteria, indicating that the protein is likely expressed uniquely during infection in a host organism.

Immunological Value of LigA

  • Strong recognition of LigA by horse serum collected from animals naturally infected with leptospira suggests that the protein is a significant target of the immune response to infection.
  • The authors propose that LigA’s infectious-stage specific expression and its immunogenic properties make it a potentially valuable tool for serodiagnosis of leptospira infections or as a component of a vaccine intended to create immunity against this bacterium.

Cite This Article

APA
Palaniappan RU, Chang YF, Jusuf SS, Artiushin S, Timoney JF, McDonough SP, Barr SC, Divers TJ, Simpson KW, McDonough PL, Mohammed HO. (2002). Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans. Infect Immun, 70(11), 5924-5930. https://doi.org/10.1128/IAI.70.11.5924-5930.2002

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 70
Issue: 11
Pages: 5924-5930

Researcher Affiliations

Palaniappan, Raghavan U M
  • College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Chang, Yung-Fu
    Jusuf, S S D
      Artiushin, S
        Timoney, John F
          McDonough, Sean P
            Barr, Steve C
              Divers, Thomas J
                Simpson, Kenneth W
                  McDonough, Patrick L
                    Mohammed, Hussni O

                      MeSH Terms

                      • Amino Acid Sequence
                      • Animals
                      • Antibodies, Bacterial / blood
                      • Bacterial Proteins / analysis
                      • Bacterial Proteins / genetics
                      • Bacterial Proteins / immunology
                      • Base Sequence
                      • Cloning, Molecular
                      • Cricetinae
                      • Escherichia coli / genetics
                      • Immunohistochemistry
                      • Leptospira interrogans / chemistry
                      • Leptospira interrogans / immunology
                      • Molecular Sequence Data
                      • Polymerase Chain Reaction
                      • Rabbits

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