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Veterinary microbiology2015; 176(1-2); 172-178; doi: 10.1016/j.vetmic.2015.01.008

Cloning and nucleotide sequence analyses of 11 genome segments of two American and one British equine rotavirus strains.

Abstract: Group A equine rotavirus (ERV) is the main cause of diarrhea in foals and causes severe economic loss due to morbidity and mortality on stud farming worldwide. Molecular evolution of equine rotaviruses remains understudies. In this study, whole-genomic analysis of 2 group A ERV, FI-14 (G3P[12]), H-2 (G3P[12]) isolated from American, and FI23 (G14P[12]) from British was carried out and genotype constellations were determined as G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 for FI-14; G14-P[12]-I2-R2-C2-M3-A10-N2-T3-E2-H7 for FI23; and G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 for H-2, respectively. With the exception of the VP7 and VP6 gene, 2 G3P[12] strains (FI-14 and H-2) and one G14P[12] strain (FI23) were highly related genetically. Of note, the VP6 genotype of H-2 strain was previously reported to be I2, however, sequence and phylogenetic analyses demonstrated that it was I6. Therefore, it showed that G3P[12] ERV strains and G14P[12] ERV strains bore a distinct VP6 genotype: I6 for G3P[12] strains and I2 for G14P[12] strains. Moreover, it demonstrated that T-cell epitope 299P-300P/Q residues (PP/Q) of VP6 may be considered as I2 ERV typical molecular marker, which facilitates the analysis of the molecular evolution of equine rotaviruses.
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Publication Date: 2015-01-15 PubMed ID: 25631250PubMed Central: PMC4329044DOI: 10.1016/j.vetmic.2015.01.008Google Scholar: Lookup
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Summary

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The research article is about the genomic analysis of two American and one British equine rotavirus strains, showing specific genotypes for each and suggesting potential molecular markers for virus evolution study.

Genome Analysis of Equine Rotavirus Strains

The study involves a comprehensive analysis of genome sequences from three group A equine rotavirus (ERV) strains: FI-14 and H-2 from America, and FI23 from Britain. ERV is a major cause of diarrhea in foals and leads to considerable economic losses due to high morbidity and mortality rates in stud farming worldwide. The primary objective of the study is to understand the molecular evolution of these rotaviruses, which has not been thoroughly explored.

  • The researchers sequenced all 11 genome segments of these rotavirus strains, identifying their genotype constellations.
  • FI-14 and H-2, both G3P[12] strains, bore similar genetic profiles across their genomes, except for the VP7 and VP6 genes.
  • The British FI23 strain, a G14P[12] virus, showed significant genetic similarity to the American strains, albeit with a different G14 genotype.

Identification of a Distinct VP6 Genotype

Through sequence and phylogenetic analysis, the VP6 gene of the H-2 American strain was reclassified from I2, as reported in previous literature, to I6. This discrepancy indicates that the G3P[12] and G14P[12] strains exhibit distinct VP6 genotypes – I6 for G3P[12] strains and I2 for G14P[12] strains. This distinct VP6 genotype distribution might have implications in the virus’s evolutionary patterns and host responses.

  • The researchers proposed T-cell epitope 299P-300P/Q (PP/Q) residues of the VP6 gene as a possible molecular marker for I2 ERV strains.
  • Identifying this molecular marker could enhance the analysis of the molecular evolution of equine rotaviruses by offering a reliable frame of reference in studying genetic changes.

Cite This Article

APA
Ma Y, Wen X, Hoshino Y, Yuan L. (2015). Cloning and nucleotide sequence analyses of 11 genome segments of two American and one British equine rotavirus strains. Vet Microbiol, 176(1-2), 172-178. https://doi.org/10.1016/j.vetmic.2015.01.008

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 176
Issue: 1-2
Pages: 172-178
PII: S0378-1135(15)00025-5

Researcher Affiliations

Ma, Yongping
  • Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, 400016 Chongqing, China; Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA.
Wen, Xiaobo
  • Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Hoshino, Yasutaka
  • Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA.
Yuan, L
  • Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA. Electronic address: lyuan@vt.edu.

MeSH Terms

  • Animals
  • Genotype
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses / genetics
  • Nucleotides / genetics
  • Phylogeny
  • RNA, Viral / genetics
  • Rotavirus / genetics
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / veterinary
  • Rotavirus Infections / virology
  • United Kingdom / epidemiology
  • United States / epidemiology

Grant Funding

  • Z99 AI999999 / Intramural NIH HHS

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Citations

This article has been cited 3 times.
  1. Pathak A, Gulati BR, Maan S, Mor S, Kumar D, Soman R, Punia S, Chaudhary D, Khurana SK. Complete Genome Sequencing Reveals Unusual Equine Rotavirus A of Bat Origin from India. J Virol 2022 Oct 26;96(20):e0140822.
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  2. Nemoto M, Matsumura T. Equine rotavirus infection. J Equine Sci 2021 Mar;32(1):1-9.
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  3. Azevedo LS, Costa FF, Ghani MBA, Viana E, França Y, Medeiros RS, Guiducci R, Morillo SG, Primo D, Lopes RD, Gomes-Gouvêa MS, da Costa AC, Luchs A. Full genotype characterization of Brazilian canine G3P[3] strains during a 10-year survey (2012-2021) of rotavirus infection in domestic dogs and cats. Arch Virol 2023 Jun 12;168(7):176.
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