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Home / Equine Research Bank / Mem Inst Oswaldo Cruz / Clostridium difficile ribotypes in humans and animals in Bra...
Memorias do Instituto Oswaldo Cruz2015; 110(8); 1062-1065; doi: 10.1590/0074-02760150294

Clostridium difficile ribotypes in humans and animals in Brazil.

Abstract: Clostridium difficile is an emerging enteropathogen responsible for pseudomembranous colitis in humans and diarrhoea in several domestic and wild animal species. Despite its known importance, there are few studies about C. difficile polymerase chain reaction (PCR) ribotypes in Brazil and the actual knowledge is restricted to studies on human isolates. The aim of the study was therefore to compare C. difficile ribotypes isolated from humans and animals in Brazil. Seventy-six C. difficile strains isolated from humans (n = 25), dogs (n = 23), piglets (n = 12), foals (n = 7), calves (n = 7), one cat, and one manned wolf were distributed into 24 different PCR ribotypes. Among toxigenic strains, PCR ribotypes 014/020 and 106 were the most common, accounting for 14 (18.4%) and eight (10.5%) samples, respectively. Fourteen different PCR ribotypes were detected among human isolates, nine of them have also been identified in at least one animal species. PCR ribotype 027 was not detected, whereas 078 were found only in foals. This data suggests a high diversity of PCR ribotypes in humans and animals in Brazil and support the discussion of C. difficile as a zoonotic pathogen.
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Publication Date: 2015-12-11 PubMed ID: 26676318PubMed Central: PMC4708028DOI: 10.1590/0074-02760150294Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the study conducted to compare the types of Clostridium Difficile, a disease-causing bacteria, found in humans and various animals in Brazil. This was done to understand the diversity of the bacteria’s ribotypes and assess the possibility of it being a zoonotic pathogen.

Objective and Methodology

  • This research aimed to compare the ribotypes (genetic strains) of Clostridium difficile (C. difficile), a bacteria causing digestive issues, isolated from humans and various domestic and wild animal species in Brazil.
  • The study analyzed 76 C.difficile strains obtained from 25 humans, 23 dogs, 12 piglets, 7 foals, 7 calves, one cat, and one manned wolf for their polymerase chain reaction (PCR) ribotypes. PCR is a biotechnological process that copies DNA to identify strains of bacteria.

Results and Findings

  • A total of 24 different PCR ribotypes of C.difficile were identified from the collected samples.
  • The most common toxin-producing strains among these were PCR ribotypes 014/020 and 106. They were found in 18.4% and 10.5% of the samples, respectively.
  • In the samples taken from humans, 14 different PCR ribotypes were detected. Among them, 9 ribotypes were also found in at least one species of animal.
  • PCR ribotype 027, a virulent strain associated with outbreaks of C.difficile-associated disease, was not detected in any of the samples. Ribotype 078 was only found in foals (young horses).

Implications of the Study

  • The findings indicate a high diversity of C. difficile PCR ribotypes in Brazil, suggesting a widespread occurrence among both human and different animal species.
  • The similar strain types found in humans and animals hint at C. difficile being a zoonotic pathogen – a type of pathogen that can be passed from animals to humans. The presence of C.difficile in many species supports the discussion and further research on this aspect for the bacterial transfer.

Cite This Article

APA
Silva RO, Rupnik M, Diniz AN, Vilela EG, Lobato FC. (2015). Clostridium difficile ribotypes in humans and animals in Brazil. Mem Inst Oswaldo Cruz, 110(8), 1062-1065. https://doi.org/10.1590/0074-02760150294

Publication

Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
NlmUniqueID: 7502619
Country: Brazil
Language: English
Volume: 110
Issue: 8
Pages: 1062-1065
PII: S0074-02762015005026105

Researcher Affiliations

Silva, Rodrigo Otávio Silveira
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Rupnik, Maja
  • Faculty of Medicine, University of Maribor, Maribor, Slovenia.
Diniz, Amanda Nádia
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Vilela, Eduardo Garcia
  • Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Lobato, Francisco Carlos Faria
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

MeSH Terms

  • Animals
  • Brazil
  • Cats
  • Cattle
  • Clostridioides difficile / classification
  • Clostridioides difficile / genetics
  • Clostridioides difficile / isolation & purification
  • Databases, Chemical
  • Dogs
  • Feces / microbiology
  • Horses
  • Humans
  • Polymerase Chain Reaction
  • RNA, Bacterial
  • Ribotyping
  • Swine
  • Wolves

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Citations

This article has been cited 13 times.
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