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Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue.
Abstract: Oviductal and uterine embryos were collected from mares at 5 to 7 days following ovulation 1) to evaluate the effects of oviductal tissue explants on in vitro growth and development of equine embryos and 2) to study the morphologic development of equine embryos in culture. Embryos were incubated for 5 days in a medium (control group) or in medium supplemented with oviductal tissue explants (co-culture group). Embryos were evaluated and the media changed daily. Following 5 days in culture, 10 10 (100%) control embryos and 27 29 (93%) co-cultured embryos had doubled in diameter. All embryos that were recovered as morulae developed to the blastocyst stage in culture. By 5 days in culture, 6 10 (60%) control embryos and 19 29 (66%) co-cultured embryos had reached the hatching blastocyst stage of development. By 3 days in culture, significantly more (P<0.05) control embryos versus co-cultured embryos had degenerated (4 10 vs 2 29 , respectively). By 5 days in culture, significantly more (P<0.01) control embryos versus co-cultured embryos had degenerated (6 10 vs. 3 29 , respectively). Embryos cultured with oviductal tissue were sustained longer than embryos cultured in medium alone. Hatching was characterized by the blastocyst squeezing through a small opening in the zona pellucida or by the zona pellucida thinning over approximately half of the blastocyst surface and subsequently disappearing entirely.
Publication Date: 1991-11-01 PubMed ID: 16727050DOI: 10.1016/0093-691x(91)90347-gGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research is centered around studying the effects of oviductal tissue in the growth and morphologic development of equine embryos in vitro, and it indicates that incorporating oviductal tissue in the culture medium can potentially enhance embryo survival and development.
Introduction
This study involved observing the effects of oviductal tissue explants on equine embryos’ growth and development when cultured in vitro. Its focus was primarily on assessing the morphological developments of the embryos. The embryos were collected from mares between the 5th and 7th days post ovulation and then incubated for 5 days either in a medium alone (control group) or a medium supplemented with oviductal tissue explants (co-culture group).
Methodology and Results
- Embryos were evaluated and the media changed daily for both the control and co-culture groups.
- After being cultured for 5 days, all embryos that were found as morulae developed to the blastocyst stage.
- It was observed that 60% of the control embryos and 66% of the co-cultured embryos had reached the hatching blastocyst stage of development by the 5th day of being cultured.
- On the 3rd day of culture, more control embryos had degenerated compared to the co-cultured embryos. This difference became even larger by the 5th day of culture, indicating that the addition of oviductal tissue in the culture medium improved embryo viability.
- Moreover, the co-cultured embryos exhibited enhanced longevity compared to those grown in medium alone.
Hatching Process
In this study, the hatching process was characterized by two distinct phenomena. Either by the blastocyst squeezing through an opening in the zona pellucida or by the zona pellucida thinning across half of the blastocyst surface and subsequently disappearing entirely.
Conclusions
Overall, the study suggests that utilizing oviductal tissue explants in the culture medium of in vitro equine embryos may result in better embryo survival and morphologic development. The research indicates that the oviductal tissue can play a critical role in promoting the growth and development of equine embryos.
Cite This Article
APA
Freeman DA, Butler JE, Weber JA, Geary RT, Woods GL.
(1991).
Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue.
Theriogenology, 36(5), 815-822.
https://doi.org/10.1016/0093-691x(91)90347-g Publication
Researcher Affiliations
- Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science University of Idaho Moscow, ID 83843 USA.
Citations
This article has been cited 1 times.- Iqbal K, Chitwood JL, Meyers-Brown GA, Roser JF, Ross PJ. RNA-seq transcriptome profiling of equine inner cell mass and trophectoderm. Biol Reprod 2014 Mar;90(3):61.
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