Cobalt metabolism in horse. Serum level and biosynthesis of vitamin B12.
Abstract: The levels of serum vitamin B were determined on 16 mature partly warm-blooded, partly Finnish rural-race horses by the radioisotopic competitive inhibition assay method. The mean value from three samplings carried out in dupli- or triplicate was 1.54 ± 0.16 ng/ml. The utilization of serum inorganic cobalt for cyanocobalamin synthesis was studied on two geldings, which received a dose of 200 µCi CoGl i.v. A Sephadex G-100 gel filtration was carried out with the serum proteins from serial blood samplings at different time intervals 15 min. to 48 hrs. after administration. The gel filtration showed the presence of two labelled proteins in the serum, one of them appearing some time after administration and disappearing almost completely towards the end of the experimental period. The two elution peaks are considered to represent inorganic Co and Co labelled vitamin B. The appearance of labelling in serum vitamin B indicates the passing of cobalt into the intestine, and reabsorption into blood in the form of vitamin B. Koncentrationen av vitamin B i serum bestämdes hos 16 vuxna delvis varmbio diga, delvis finska hästar med hjälp av den kompetitiva radioisotop-inhibitionsmetoden. Medelvärdet av tre dubbelt eller tredubbelt undersökta prov var 1,54 ± 0,16 ng/ml. Utnyttjandet av serum oorganiskt kobolt för biosyntes av cyanokobalamin undersöktes hos två vallacker, som fick en dos av 200 µCi CoCl intravenöst. En Sephadex G-100 gelfiltration utfördes med serumproteiner från en serie av blodprov tagna 15 min. till 48 timmar efter injektionen. Gelfiltrationen visade, att det fanns två märkta proteiner i serum varvid den ena av dessa uppkom först en tid efter injektionen och försvann nästan helt mot slutet av försöksperioden. De två elutionstopparna ansågs representera oorganiskt Go och med Go märkt vitamin B. Uppkomsten av märkning serum vitamin B visar, att kobolt har passerat in i tarminnehållet, och reabsorberat i form av vitamin B.
Publication Date: 1975-01-01 PubMed ID: 1136912PubMed Central: PMC8396007DOI: 10.1186/BF03546698Google Scholar: Lookup
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Summary
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This study investigates the level of serum vitamin B and the use of serum inorganic cobalt for vitamin B12 synthesis in horses. The researchers showed that cobalt can enter the intestines and be reabsorbed back into the bloodstream as vitamin B.
Analysis of Serum Vitamin B Level
- The research was carried out on 16 mature horses of mixed breeds. Their serum vitamin B level was determined using a radioisotopic competitive inhibition assay, a laboratory method which employs radioisotopes to analyse the presence and concentration of biological molecules.
- Multiple tests were carried out, yielding a mean vitamin B level of 1.54 ± 0.16 ng/ml. This result provides a baseline of serum vitamin B level in the horses under study.
Investigation of Cobalt Utilization
- The researchers further studied the synthesis of cyanocobalamin, a form of vitamin B12, from serum inorganic cobalt. They administered 200 µCi of CoCl (Cobalt Chloride) intravenously to two geldings, a type of castrated male horse.
- Subsequent analysis of the horse’s serum at various minutes or hours post-administration was carried out using Sephadex G-100 gel filtration. This advanced laboratory technique helps in the separation and analysis of proteins and other macromolecules in a serum sample.
Results and Interpretation
- Results from the gel filtration revealed the presence of two labelled proteins. One appeared after the administration of CoCl and gradually disappeared as the experimental period progressed. The researchers believe the two peaks observed represent inorganic cobalt and cobalt labelled vitamin B.
- The transient appearance of cobalt labelled vitamin B in the serum supports the idea that cobalt can transition into the intestine and then be reabsorbed into the blood as vitamin B. This suggests that the body utilizes ingested cobalt to synthesize vitamin B12.
Cite This Article
APA
Salminen K.
(1975).
Cobalt metabolism in horse. Serum level and biosynthesis of vitamin B12.
Acta Vet Scand, 16(1), 84-94.
https://doi.org/10.1186/BF03546698 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cobalt / metabolism
- Cobalt Radioisotopes
- Horses
- Male
- Vitamin B 12 / biosynthesis
- Vitamin B 12 / blood
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