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Animal reproduction science2019; 210; 106195; doi: 10.1016/j.anireprosci.2019.106195

Combined addition of superoxide dismutase, catalase and glutathione peroxidase improves quality of cooled stored stallion semen.

Abstract: During cold storage stallion spermatozoa experience undergo oxidative stress, which can impair sperm function and fertilizing capacity. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) are the main endogenous enzymatic antioxidants in stallion seminal plasma, and counteract reactive oxygen species. Semen dilution reduces the endogenous antioxidant concentrations. The aim of this study was to investigate whether addition of 15 IU/mL each of SOD, CAT, and GPX to diluted stallion semen would ameliorate a reactive oxygen-mediated decrease in semen quality during 72 h of storage at 5 °C. Ejaculates (n = 7) were divided in two aliquots and diluted in INRA 96 without (control) or with addition of antioxidants. Semen analysis was performed at the time of dilution and every 24 h during chilled storage. Antioxidant supplementation completely inhibited the storage-dependent increase in activated caspase 3 (P < 0.05). Concomitantly, the antioxidant-supplemented samples had a greater percentage of viable, motile and rapidly moving sperm than control samples after 72 h storage (P < 0.05). The DNA damage, as evaluated by TUNEL assay and SCSA, increased with storage time (P < 0.05). Antioxidant supplementation did not prevent, but did significantly reduce the increase in DNA strand breakage. The results indicate part of the intrinsic apoptotic pathway leading to effector caspase activation was inhibited, although an activation of molecules with endonuclease activity still occurred. In conclusion, adding equal concentrations of SOD, CAT and GPX to a semen extender suppressed caspase-3 activation and improved preservation of stallion sperm motility and viability during 72 h of storage at 5 °C.
Copyright © 2019 Elsevier B.V. All rights reserved.
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Publication Date: 2019-09-23 PubMed ID: 31635777DOI: 10.1016/j.anireprosci.2019.106195Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how adding antioxidant enzymes to stallion semen during cold storage can prevent oxidative stress and enhance the longevity and quality of the semen.

Underlying Problem

  • When stallion sperm is stored in cold conditions, it can experience oxidative stress. Oxidative stress harms sperm function and can reduce the ability of the sperm to fertilize an egg.
  • As semen is diluted for storage, it loses much of its natural antioxidants – superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX).

Aim of the Study

  • The researchers purpose is to examine if the decline in semen quality during cold storage can be mitigated by adding an equal combination of SOD, CAT, and GPX to the semen.

Study Methodology

  • Seven ejaculates were collected and divided into two sample batches.
  • One batch was stored with added antioxidants and the other served as a control with no antioxidants added.
  • Semen samples were analyzed at the start of the study and every 24 hours during chilled storage for 72 hours.

Findings

  • The addition of the antioxidants inhibited the activation of caspase 3, a protein that typically marks the early stages of cell apoptosis (cell death).
  • Samples with added antioxidants had a significantly higher percentage of viable, motile and rapidly moving sperm than the control samples after 72 hours.
  • Although DNA damage did occur with storage time, antioxidant supplementation significantly reduced the increase in DNA strand breakage.

Conclusion

  • The study showed that adding equal concentrations of SOD, CAT and GPX to diluted semen suppressed caspase-3 activation and enhanced the preservation of stallion sperm’s motility and viability during 72 hours of storage at 5°C.

Cite This Article

APA
Del Prete C, Stout T, Montagnaro S, Pagnini U, Uccello M, Florio P, Ciani F, Tafuri S, Palumbo V, Pasolini MP, Cocchia N, Henning H. (2019). Combined addition of superoxide dismutase, catalase and glutathione peroxidase improves quality of cooled stored stallion semen. Anim Reprod Sci, 210, 106195. https://doi.org/10.1016/j.anireprosci.2019.106195

Publication

Animal reproduction science
ISSN: 1873-2232
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 210
Pages: 106195
PII: S0378-4320(18)31018-2

Researcher Affiliations

Del Prete, Chiara
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy. Electronic address: chiara.delprete@unina.it.
Stout, Tom
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM, Utrecht, the Netherlands.
Montagnaro, Serena
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Pagnini, Ugo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Uccello, Melania
  • Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Napoli, Italy.
Florio, Pasquale
  • Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Napoli, Italy.
Ciani, Francesca
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Tafuri, Simona
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Palumbo, Veronica
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Pasolini, Maria Pia
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Cocchia, Natascia
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino, 1, 80137, Napoli, Italy.
Henning, Heiko
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM, Utrecht, the Netherlands.

MeSH Terms

  • Animals
  • Catalase / administration & dosage
  • Catalase / pharmacology
  • Glutathione Peroxidase / administration & dosage
  • Glutathione Peroxidase / pharmacology
  • Horses / physiology
  • Male
  • Semen / drug effects
  • Semen Analysis / veterinary
  • Semen Preservation / veterinary
  • Sperm Motility
  • Spermatozoa / physiology
  • Superoxide Dismutase / administration & dosage
  • Superoxide Dismutase / pharmacology

Citations

This article has been cited 12 times.
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