Combined use of platelet rich plasma and vitamin C positively affects differentiation in vitro to mesodermal lineage of adult adipose equine mesenchymal stem cells.
Abstract: Repair of injured soft and hard tissues in horses can benefit greatly from the use of regenerative therapies with mesenchymal stem cells (MSC). Vitamin-C and platelet-rich-plasma had been used for in vitro differentiation of MSC. This study was aimed to evaluate the effect of vitamin-C, platelet-rich-plasma and their combination on the in vitro differentiation of adipose horse MSC. We isolated MSC from horse fat and differentiated them in vitro into osteogenic and chondrogenic lineages, as demonstrated by specific staining and RT-qPCR of selected genes. Combining vitamin-C and plasma-rich-platelet positively affected the ability of MSC to differentiate in vitro into mesodermal lineages during 14 days of culture; this effect was not as marked when differentiation was attempted for 21 days. This provides valuable information on the effect of combined use of these molecules in regenerative therapies and their potential application along stem cells for lesions of musculoskeletal tissue in sport horses.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication Date: 2013-12-13 PubMed ID: 24377415DOI: 10.1016/j.rvsc.2013.12.005Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper discusses how the combined use of platelet rich plasma and vitamin C can improve the in vitro differentiation of horse mesenchymal stem cells for potential applications in regenerative therapies.
Overview of the Study
- The study primarily focuses on the repair of injured tissues in horses using regenerative therapies with mesenchymal stem cells (MSC).
- These stem cells were isolated specifically from adipose (fat) tissue of horses.
- The use of both platelet rich plasma and Vitamin C for inducing in vitro differentiation of these stem cells was evaluated.
- Two types of biological lineages were targeted for the differentiation process – osteogenic and chondrogenic lineages.
Methodology and Findings
- Specific staining and RT-qPCR of selected genes were used to demonstrate the differentiation of the stem cells into osteogenic and chondrogenic lineages.
- When used in combination, Vitamin C and platelet rich plasma were found to have a positive effect on the differentiation ability of the MSCs.
- This effect, however, was prominent only during the initial 14 days of the culture and decreased when the differentiation was attempted for a longer period of 21 days.
Implications of the Study
- The results of this study provide valuable insight into the potential applications of MSCs in regenerative therapies, specifically in healing tissue damage in sport horses.
- It is also notable that the efficiency of differentiation with the combined use of these molecules decreases over time, indicating a need for further research on sustained differentiation strategies.
Cite This Article
APA
Castro FO, Torres A, Cabezas J, Rodríguez-Alvarez L.
(2013).
Combined use of platelet rich plasma and vitamin C positively affects differentiation in vitro to mesodermal lineage of adult adipose equine mesenchymal stem cells.
Res Vet Sci, 96(1), 95-101.
https://doi.org/10.1016/j.rvsc.2013.12.005 Publication
Researcher Affiliations
- Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile. Electronic address: fidcastro@udec.cl.
- Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
- Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
- Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
MeSH Terms
- Adipose Tissue / cytology
- Adipose Tissue / metabolism
- Animals
- Ascorbic Acid / metabolism
- Cell Differentiation / genetics
- Cell Differentiation / physiology
- Core Binding Factor Alpha 1 Subunit / genetics
- Core Binding Factor Alpha 1 Subunit / metabolism
- Female
- Histocytochemistry / veterinary
- Horses / metabolism
- Hyaluronan Receptors / genetics
- Hyaluronan Receptors / metabolism
- Integrin beta1 / genetics
- Integrin beta1 / metabolism
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / metabolism
- Osteogenesis / physiology
- Osteonectin / genetics
- Osteonectin / metabolism
- Platelet-Rich Plasma / metabolism
- RNA / chemistry
- RNA / genetics
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- SOX9 Transcription Factor / genetics
Citations
This article has been cited 10 times.- Taihi I, Pilon C, Cohen J, Berdal A, Gogly B, Nassif A, Fournier BP. Efficient isolation of human gingival stem cells in a new serum-free medium supplemented with platelet lysate and growth hormone for osteogenic differentiation enhancement. Stem Cell Res Ther 2022 Mar 25;13(1):125.
- Bukowska J, Szóstek-Mioduchowska AZ, Kopcewicz M, Walendzik K, Machcińska S, Gawrońska-Kozak B. Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science. Stem Cell Rev Rep 2021 Jun;17(3):719-738.
- Echeverry DM, Asenjo PA, Rojas DM, Aguilera CJ, Rodríguez-Álvarez L, Castro FO. Characterization of mesenchymal stem cells derived from adipose tissue of a cougar (Puma concolor). Anim Reprod 2020 May 5;17(2):e20190109.
- Mançanares ACF, Cabezas J, Manríquez J, de Oliveira VC, Wong Alvaro YS, Rojas D, Navarrete Aguirre F, Rodriguez-Alvarez L, Castro FO. Edition of Prostaglandin E2 Receptors EP2 and EP4 by CRISPR/Cas9 Technology in Equine Adipose Mesenchymal Stem Cells. Animals (Basel) 2020 Jun 23;10(6).
- Yan X, Zhou Z, Guo L, Zeng Z, Guo Z, Shao Q, Xu W. BMP7-overexpressing bone marrow-derived mesenchymal stem cells (BMSCs) are more effective than wild-type BMSCs in healing fractures. Exp Ther Med 2018 Aug;16(2):1381-1388.
- Fernandes G, Yang S. Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering. Bone Res 2016;4:16036.
- Wang Y, Yu X, Chen E, Li L. Liver-derived human mesenchymal stem cells: a novel therapeutic source for liver diseases. Stem Cell Res Ther 2016 May 12;7(1):71.
- Ye JS, Su XS, Stoltz JF, de Isla N, Zhang L. Signalling pathways involved in the process of mesenchymal stem cells differentiating into hepatocytes. Cell Prolif 2015 Apr;48(2):157-65.
- Sawicki S, Bugno-Poniewierska M, Żurowski J, Szmatoła T, Semik-Gurgul E, Bochenek M, Karnas E, Gurgul A. Comparative Transcriptome and MicroRNA Profiles of Equine Mesenchymal Stem Cells, Fibroblasts, and Their Extracellular Vesicles. Genes (Basel) 2025 Aug 5;16(8).
- Orazi S, Boffa A, Salerno M, Angelelli L, Zaffagnini S, Filardo G. Adipose tissue-derived injectable products combined with platelet-rich plasma for the treatment of osteoarthritis: the promising preclinical results are not confirmed by the clinical evidence. EFORT Open Rev 2024 Nov 8;9(11):1023-1033.
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