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Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe.
Abstract: The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagocytophilum was identified in the blood of 42 animals. The sequences of the 16S RNA gene amplicons that were obtained from our A. phagocytophilum isolates had 100 % similarity with each other and 96.4-100 % similarity with Anaplasma spp. sequences selected from those available in GenBank. Nucleotide substitutions at positions 248 and 249 were demonstrated in all the 16S RNA gene sequences of Anaplasma obtained in our study. This may indicate the emergence of a new rickettsial genotype that is the cause of equine granulocytic anaplasmosis in southern and eastern Europe. These results add new information on the epidemiology and genetic diversity of A. phagocytophilum detected in the blood of horses from southern and eastern Europe.
Publication Date: 2013-06-20 PubMed ID: 23788590DOI: 10.1099/jmm.0.058636-0Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research aimed to compare the genetic material of Anaplasma phagocytophilum, a bacteria found in horses that had come into contact with ticks, across different regions in Europe. It was noticed that a new interpretation of this bacteria could be responsible for causing a condition known as equine granulocytic anaplasmosis in Southern and Eastern Europe.
Research Procedure and Subjects
- The study involved 234 horses, each of which had been exposed to ticks.
- A process called Polymerase Chain Reaction (PCR) was used to identify the genetic material of Anaplasma phagocytophilum in the blood of these horses.
- Out of the 234 horses, A. phagocytophilum was detected in 42 animals.
Analysis of 16S RNA Gene Sequences
- 16S RNA gene sequences were analysed to identify the specific fragments of nucleotide sequences for A. phagocytophilum strains present in the horse’s blood.
- After analysing, it was found that the 16S RNA gene sequences in A. phagocytophilum from their sample had 100% similarity with each other.
- However, when compared with other Anaplasma sequences found in the GenBank database, the similarity ranged between 96.4% to 100%.
Discovery of a new Rickettsial Genotype
- The researchers noticed nucleotide substitutions at positions 248 and 249 in all the 16S RNA gene sequences of Anaplasma they obtained.
- These substitutions suggest the possibility of a new genotype of this bacterium emerging.
- This new genotype could be responsible for a condition known as equine granulocytic anaplasmosis, particularly prevalent in horses from southern and eastern Europe.
Significance of the Study
- This study contributes valuable new data to the understanding of the epidemiology and genetic diversity of A. phagocytophilum found in the blood of horses.
- Understanding the genetic diversity of this bacterium is crucial for detecting and managing the spread of equine granulocytic anaplasmosis.
Cite This Article
APA
Dzięgiel B, Adaszek L, Winiarczyk M, García-Bocanegra I, Carbonero A, Dębiak P, Winiarczyk S.
(2013).
Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe.
J Med Microbiol, 62(Pt 12), 1891-1896.
https://doi.org/10.1099/jmm.0.058636-0 Publication
Researcher Affiliations
- Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
- Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
- Department of Vitreoretinal Surgery, Medical University of Lublin, Chmielna 1, 20-079 Lublin, Poland.
- Departamento de Sanidad Animal, Facultad de Veterinaria, UCO Campus, Universitarios de Rabanales, 14071 Córdoba, Spain.
- Departamento de Sanidad Animal, Facultad de Veterinaria, UCO Campus, Universitarios de Rabanales, 14071 Córdoba, Spain.
- Laboratory of Radiology and Ultrasonography, Department and Clinic of Animal Surgery, University of Life Science in Lublin, Gleboka 30 St, 20-612 Lublin, Poland.
- Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 30 Głęboka St 20-612 Lublin, Poland.
MeSH Terms
- Anaplasma phagocytophilum / genetics
- Anaplasma phagocytophilum / isolation & purification
- Animals
- Base Sequence / genetics
- DNA, Bacterial / genetics
- Ehrlichiosis / microbiology
- Europe
- Genotype
- Horses
- Nucleotides / genetics
- Phylogeny
- RNA, Ribosomal, 16S / genetics
- Ticks / microbiology
Citations
This article has been cited 4 times.- Bogdan AM, Mitrea IL, Ionita M. Equine Granulocytic Anaplasmosis: A Systematic Review and Meta-Analysis on Clinico-Pathological Findings, Diagnosis, and Therapeutic Management. Vet Sci 2024 Jun 13;11(6).
- Fuehrer HP, Alho AM, Kayikci FN, Shahi Barogh B, Rosa H, Tomás J, Rocha H, Harl J, Madeira de Carvalho L. Survey of Zoonotic and Non-zoonotic Vector-Borne Pathogens in Military Horses in Lisbon, Portugal. Front Vet Sci 2020;7:591943.
- Dzięgiel B, Adaszek Ł, Carbonero A, Łyp P, Winiarczyk M, Dębiak P, Winiarczyk S. Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR. Parasitol Res 2016 Mar;115(3):1039-44.
- Savidge C, Ewing P, Andrews J, Aucoin D, Lappin MR, Moroff S. Anaplasma phagocytophilum infection of domestic cats: 16 cases from the northeastern USA. J Feline Med Surg 2016 Feb;18(2):85-91.
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