Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples.
Abstract: In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. Methods: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. Results: Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. Conclusions: Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.
© 2017 John Wiley & Sons Ltd.
Publication Date: 2017-02-21 PubMed ID: 28220614DOI: 10.1111/vco.12308Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research article discusses a comparative analysis of DNA methylation patterns between equine sarcoid (a type of skin tumor in horses) and healthy skin samples. The study identified differentially methylated genomic regions, many of which were located within genes related to oncogenesis. This information could potentially help to develop new diagnostic or therapeutic approaches to equine sarcoids.
Methods
- The researchers used the Reduced Representation Bisulfite Sequencing (RRBS) technique to detect and analyze the DNA methylation patterns. RRBS is a method that can examine methylation patterns on a genome-wide scale.
- Through this technique, they identified 136 regions showing differential methylation between sarcoid and normal skin tissue.
- Most of the differentially methylated genomic regions (DMRs) identified were short fragments, less than 1kb in size, and located in the intergenic regions. Intergenic regions are spaces in the genome that are located between genes.
Results
- Among the identified DMRs, some regions were located within genes that are directly or indirectly related to oncogenesis, the process of tumor formation.
- They validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in the lesional sample. CpG sites are regions of DNA where a cytosine nucleotide is followed by a guanine nucleotide.
- Hypomethylation is a decrease in the level of methylation, while hypermethylation is an increase in the level of methylation.
- The changes in the DNA methylation at these sites confirmed the previously identified changes.
Conclusions
- The researchers concluded that understanding the changes that occur in DNA methylation in equine sarcoids could provide valuable insights for the development of new alternative diagnostic or therapeutic approaches.
- The results indicate that differences in DNA methylation patterns between healthy and tumor tissues might play a crucial role in the development of the disease.
Cite This Article
APA
Semik E, Ząbek T, Gurgul A, Fornal A, Szmatoła T, Pawlina K, Wnuk M, Klukowska-Rötzler J, Koch C, Mählmann K, Bugno-Poniewierska M.
(2017).
Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples.
Vet Comp Oncol, 16(1), 37-46.
https://doi.org/10.1111/vco.12308 Publication
Researcher Affiliations
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- Department of Genetics, Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Rzeszow, Poland.
- Division of Pedriatric Hematology/Oncology, Department of Clinical Research, University of Bern, Bern, Switzerland.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Bern, Switzerland.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Bern, Switzerland.
- Swiss Institute of Equine Medicine ISME, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern and Agroscope, Bern, Switzerland.
- Equine Clinic, General Surgery and Radiology, Freie Universität Berlin, Berlin, Germany.
- Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
MeSH Terms
- Animals
- DNA Methylation
- DNA, Neoplasm / metabolism
- Horse Diseases / metabolism
- Horses
- Skin / metabolism
- Skin Neoplasms / metabolism
- Skin Neoplasms / veterinary
Citations
This article has been cited 3 times.- Semik-Gurgul E, Gurgul A, Szmatoła T. Transcriptome and methylome sequencing reveals altered long non-coding RNA genes expression and their aberrant DNA methylation in equine sarcoids.. Funct Integr Genomics 2023 Aug 8;23(3):268.
- Pawlina-Tyszko K, Semik-Gurgul E, Ząbek T, Witkowski M. Methylation Status of Gene Bodies of Selected microRNA Genes Associated with Neoplastic Transformation in Equine Sarcoids.. Cells 2022 Jun 14;11(12).
- Unger L, Jagannathan V, Pacholewska A, Leeb T, Gerber V. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression.. J Vet Intern Med 2019 Jan;33(1):241-250.
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