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Animals : an open access journal from MDPI2024; 14(12); doi: 10.3390/ani14121717

Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses.

Abstract: Testicular development and spermatogenesis are tightly regulated by both coding and non-coding genes, with mRNA and lncRNA playing crucial roles in post-transcriptional gene expression regulation. However, there are significant differences in regulatory mechanisms before and after sexual maturity. Nevertheless, the mRNAs and lncRNAs in the testes of Mongolian horses have not been systematically identified. In this study, we first identified the testicular tissues of sexually immature and sexually mature Mongolian horses at the tissue and protein levels, and comprehensively analyzed the expression profiles of mRNA and lncRNA in the testes of 1-year-old (12 months, n = 3) and 10-year-old (n = 3) Mongolian horses using RNA sequencing technology. Through gene expression analysis, we identified 16,582 mRNAs and 2128 unknown lncRNAs that are commonly expressed in both sexually immature and sexually mature Mongolian horses. Meanwhile, 9217 mRNAs (p < 0.05) and 2191 unknown lncRNAs (p < 0.05) were identified as differentially expressed between the two stages, which were further validated by real-time fluorescent quantitative PCR and analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The analysis results showed that genes in the sexually immature stage were mainly enriched in terms related to cellular infrastructure, while genes in the sexually mature stage were enriched in terms associated with hormones, metabolism, and spermatogenesis. In summary, the findings of this study provide valuable resources for a deeper understanding of the molecular mechanisms underlying testicular development and spermatogenesis in Mongolian horses and offer new perspectives for future related research.
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Publication Date: 2024-06-07 PubMed ID: 38929336PubMed Central: PMC11200857DOI: 10.3390/ani14121717Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study primarily focuses on an in-depth comparison of mRNA and lncRNA expression profiles in testicular tissue of both sexually immature and sexually mature Mongolian horses. The aim is to enhance our understanding about the molecular mechanisms that facilitate testicular development and spermatogenesis with a potential to contribute to future relevant research.

Objective and Approach

  • The objective was to systematically identify and compare mRNAs and lncRNAs in the testes of Mongolian horses before and after they reach sexual maturity.
  • The researchers used RNA sequencing technologies to thoroughly examine the expression profiles of mRNA and lncRNA in the testes of Mongolian horses aged 1 (sexually immature) and 10 (sexually mature) years.

Main Findings

  • Through gene expression analysis, the study identified 16,582 mRNA and 2128 unknown lncRNA that were commonly expressed in both sexually immature and sexually mature Mongolian horses.
  • Differential gene expression was noted between the two stages i.e., 9217 mRNAs, and 2191 unknown lncRNAs were differently expressed. These findings were validated using real-time fluorescent quantitative PCR.

Analysis and Conclusion

  • The findings were analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG).
  • The analysis showed the genes in sexually immature stage were mainly associated with terms related to the cellular infrastructure, while those in the sexually mature stage were linked to hormones, metabolism, and spermatogenesis.
  • Thus, the study provides valuable insights into understanding the molecular mechanisms of testicular development and spermatogenesis in Mongolian horses and could potentially inform future research in this area.

Cite This Article

APA
Liu Y, Du M, Zhang L, Wang N, He Q, Cao J, Zhao B, Li X, Li B, Bou G, Zhao Y, Dugarjaviin M. (2024). Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses. Animals (Basel), 14(12). https://doi.org/10.3390/ani14121717

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 12

Researcher Affiliations

Liu, Yuanyi
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Du, Ming
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhang, Lei
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wang, Na
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
He, Qianqian
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Cao, Jialong
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhao, Bilig
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Li, Xinyu
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Li, Bei
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bou, Gerelchimeg
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhao, Yiping
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dugarjaviin, Manglai
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.

Grant Funding

  • U23A20224 / Key Support Project of the National Natural Science Joint Fund
  • BR221208 / Operating expenses of basic scientific research project of IMAU
  • QT202215 / High level Achievement Cultivation Project of the College of Animal Science, Inner Mongolia Agricultural University
  • CG202445 / Special Project for Cultivating High-level Achievements of the College of Animal Science, Inner Mongolia Agricultural University

Conflict of Interest Statement

The authors declare no conflicts of interest.

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