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Home / Equine Research Bank / J Vet Diagn Invest / Comparative evaluation of Rose Bengal plate agglutination te...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2007; 19(4); 362-367; doi: 10.1177/104063870701900404

Comparative evaluation of Rose Bengal plate agglutination test, mallein test, and some conventional serological tests for diagnosis of equine glanders.

Abstract: The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and other serological tests, including indirect hemagglutination test (IHAT), complement fixation test (CFT), and modified counter immunoelectrophoresis test (mCIET). Sera from 70 naturally infected culture-positive, 96 potentially exposed cohorts, and 110 healthy equines were tested. All tests but mCIET showed 100% specificity when testing the sera from glanders-negative equines. The calculated sensitivities of RBT, IHAT, CFT, mCIET, and mallein test when testing culture-positive equines were 90.0, 97.1, 91.4, 81.4, and 75.7%, respectively. The RBT was significantly (P < 0.05) more sensitive than the mallein test and mCIET. The positive and negative predictive values of each test (RBT, IHAT, CFT, mallein test, and mCIET) were as follows: 100 and 94, 100 and 98.2, 100 and 96.7, 100 and 86.6, and 90.5 and 88.6, respectively. On comparing glandered and nonglandered animals, the highest agreement (0.987) was found between RBT and CFT followed by RBT and IHAT (0.940), RBT and mallein test (0.871), and RBT and mCIET (0.852). Because the RBT is simpler and rapid to perform, the inclusion of the test as a supplementary test for the diagnosis of glanders in field conditions is recommended.
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Publication Date: 2007-07-05 PubMed ID: 17609344DOI: 10.1177/104063870701900404Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study compares the efficiency of Rose Bengal plate agglutination test (RBT) with other serological tests in diagnosing equine glanders. The research suggests that RBT is more sensitive and reliable than other tests, establishing it as a potential supplementary test for glander diagnosis in horses in field conditions.

Research and Its Methodology

  • The study evaluated the Rose Bengal Plate Agglutination Test (RBT) against other conventional serological tests like Mallein test, Indirect Hemagglutination Test (IHAT), Complement Fixation Test (CFT), and Modified Counter Immunoelectrophoresis Test (mCIET) for the diagnosis of equine glanders.
  • A total of 276 sera, originating from 70 naturally infected culture-positive equines, 96 potentially exposed cohorts and 110 healthy equines, underwent testing.

Findings of the Study

  • The tests, excluding mCIET, demonstrated 100% specificity when used to test sera from glanders-negative equines.
  • The calculated sensitivities of RBT, IHAT, CFT, mCIET, and Mallein test when applied to culture-positive equines were 90.0%, 97.1%, 91.4%, 81.4%, and 75.7%, respectively.
  • RBT was significantly more sensitive than both the mallein test and mCIET (with P < 0.05).
  • In terms of positive and negative predictive values, each test (RBT, IHAT, CFT, mallein test, and mCIET) recorded the following results: 100% and 94%, 100% and 98.2%, 100% and 96.7%, 100% and 86.6%, and 90.5% and 88.6%, respectively.

Implications of the Research

  • The RBT demonstrated high sensitivity and the highest agreement with both the CFT and IHAT tests, suggesting its effectiveness in diagnosing equine glanders.
  • Given the simplicity and speed of the RBT, the researchers recommend its inclusion as a supplementary test for diagnosing glanders in field conditions.
  • While the study suggests RBT as a potentially useful and reliable test, further studies are recommended to substantiate these findings under various field conditions and larger sample sizes.

Cite This Article

APA
Naureen A, Saqib M, Muhammad G, Hussain MH, Asi MN. (2007). Comparative evaluation of Rose Bengal plate agglutination test, mallein test, and some conventional serological tests for diagnosis of equine glanders. J Vet Diagn Invest, 19(4), 362-367. https://doi.org/10.1177/104063870701900404

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1040-6387
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 19
Issue: 4
Pages: 362-367

Researcher Affiliations

Naureen, Abeera
  • Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, 38040 Pakistan. drabeeran@yahoo.com
Saqib, Muhammad
    Muhammad, Ghulan
      Hussain, Muhammad H
        Asi, Muhammad N

          MeSH Terms

          • Agglutination Tests / veterinary
          • Animals
          • Glanders / blood
          • Glanders / diagnosis
          • Horses
          • Rose Bengal
          • Serologic Tests / veterinary

          Citations

          This article has been cited 18 times.
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