Comparative growth of different rotavirus strains in differentiated cells (MA104, HepG2, and CaCo-2).
Abstract: The production of viral antigen after infection of MA104, HepG2 (derived from human liver), and CaCo-2 (derived from human colon) cells with various cultivatable human and animal rotavirus strains was compared using immunofluorescence tests. All rotavirus strains examined expressed antigen in CaCo-2 cells and MA104 cells, but only some virus strains, namely, SA11-Cl3 (simian), RRV (simian), CU-1 (canine), and Ty1 (turkey), produced antigen in numbers of infected HepG2 cells comparable to infections in MA104 and CaCo-2 cells. Fl-14 (equine), OSU (porcine), NCDV (bovine), and Ch2 (chicken) strains were found to infect moderate numbers of HepG2 cells. Most human rotaviruses (representing viruses in serotypes 1, 2, 3, 4, 8, and 9), a simian rotavirus variant (SA11-4F), lapine (Ala, C-11 and R-2) viruses and porcine (Gottfried) virus infections resulted either in no detectable antigen or antigen synthesis in a low percentage of HepG2 cells. Human rotavirus isolates obtained from the stool specimens of an immunocompromised child with rotavirus antigen in his liver showed two different patterns of replication in HepG2 cells. Examination of the replication of a subset of viruses in the liver and intestinal tissues of orally infected suckling mice showed the CU-1 and Ty1 strains replicated well, while the OSU and human rotavirus strains did not. These results indicate that growth restriction in HepG2 cells is not serotype-specific, and growth of a virus in HepG2 cells does not necessarily correlate with the hepatotropic potential of a virus strain. Factors that may influence these differences of virus infectivity in HepG2 cells are discussed.
Publication Date: 1991-10-01 PubMed ID: 1653495DOI: 10.1016/0042-6822(91)90443-fGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This study investigates the growth of various human and animal rotavirus strains in three types of cells: MA104, HepG2 (human liver cells), and CaCo-2 (human colon cells). The paper suggests no clear correlation between a virus’s growth in HepG2 cells and its potential to infect the liver.
Research Methodology and Objectives
- The aim of the study was to compare the production of viral antigen, a substance that creates an immune response, in different cells (MA104, HepG2, and CaCo-2) when infected by different rotavirus strains.
- The rotavirus strains chosen for the study included strains from various animals (including simians, canines, turkeys, horses, pigs, cows, and chickens) and humans. The human rotavirus strains represented serotypes 1,2,3,4,8 and 9.
- The viral antigens were detected and measured using immunofluorescence tests, a laboratory technique used to identify the presence and distribution of specific proteins.
Key Findings
- The researchers discovered that all rotavirus strains examined were capable of expressing antigen in both CaCo-2 cells and MA104 cells.
- However, when it comes to HepG2 cells, only some rotavirus strains such as SA11-Cl3 (simian strain), RRV (simian strain), CU-1 (canine), and Ty1 (turkey) were found to produce antigen in significant amounts.
- Conversely, most human rotaviruses, a simian rotavirus variant (SA11-4F), lapine (Ala, C-11 and R-2) viruses, and porcine (Gottfried) virus infections resulted in either no detectable antigen or a low level of antigen synthesis in HepG2 cells.
- The growth restriction seen in HepG2 cells was not found to be serotype-specific, and the ability of a virus to grow in HepG2 cells did not necessarily suggest that the virus strain has a strong potential to infect the liver (hepatotropic potential).
Implications and Further Discussion
- This research is significant as it sheds light on the variability of rotavirus growth in different cell environments. The study demonstrates that not all strains behave the same way in all types of cells. Hence, understanding the cell-specific response to rotavirus infection can help in the development of effective therapies.
- The factors influencing the differences in virus infectivity in HepG2 cells may require further research to fully understand the infection and transmission patterns of different rotavirus strains, which could further aid in developing effective preventions and treatments.
Cite This Article
APA
Kitamoto N, Ramig RF, Matson DO, Estes MK.
(1991).
Comparative growth of different rotavirus strains in differentiated cells (MA104, HepG2, and CaCo-2).
Virology, 184(2), 729-737.
https://doi.org/10.1016/0042-6822(91)90443-f Publication
Researcher Affiliations
- Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
MeSH Terms
- Animals
- Antigens, Viral / analysis
- Cell Differentiation
- Cells, Cultured
- Diarrhea / microbiology
- Haplorhini
- Humans
- In Vitro Techniques
- Intestines / microbiology
- Kidney / microbiology
- Liver / microbiology
- Mice
- Rotavirus / growth & development
- Rotavirus / immunology
- Virus Replication
Grant Funding
- AI 20649 / NIAID NIH HHS
- AI16687 / NIAID NIH HHS
- DK 30144 / NIDDK NIH HHS
Citations
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