Comparative immunohistochemical study of stellate cells in normal canine and equine adenohypophyses and in pituitary tumours.
Abstract: The presence and distribution of S100 protein (alpha and beta subunits), cytokeratin polypeptides, glial fibrillary acidic protein, neurofilaments, vimentin, neuron specific enolase, synaptophysin, HLA class II DR antigen, and pituitary hormones (prolactin, adrenocorticotropic hormone and human chorionic gonadotrophin) in stellate cells were studied immunohistochemically in four normal canine pituitary glands, five canine pituitary adenomas, two canine pituitary carcinomas and two equine pituitary adenomas (with surrounding normal glandular tissue). Stellate cells of the pars distalis and pars intermedia of canine and equine adenohypophyses showed a strong reaction with antibodies against S100 protein subunits alpha and beta. They also reacted with antibody against high and low molecular weight cytokeratins, but not with those against other intermediate filament proteins, neuroendocrine markers, the HLA-class II DR antigen or the pituitary hormones. Other populations of cells expressing both subunits of the S100 protein were polygonal cells of the pars distalis of the adenohypophysis (horse) and marginal epithelial cells of the pars intermedia of the adenohypophysis (dog and horse). Some pituitary tumours had S100-immunoreactive cells with a distribution of alpha and beta subunits that differed between the two species. Some canine tumours (one adenoma and one carcinoma) expressed only the alpha subunit, but both of the equine adenomas expressed alpha and beta protein subunits. Some of the S100-immunoreactive tumour cells reacted with RCK-102 (cytokeratins 5+8) antibody in the dog but not in the horse. The results suggested that canine and equine stellate cells of the adenohypophysis are more closely related to epithelial than to glial cells, as is the case in cattle, sheep and goats but not human beings or mice. No subpopulation of cells of bone marrow origin could be identified among canine stellate cells, as they lack MHC class II antigen. The results also suggested that the presence of S100-immunoreactive cells is more striking in canine and equine tumours than in human tumours.
Publication Date: 1998-03-21 PubMed ID: 9500236DOI: 10.1016/s0021-9975(98)80025-xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article presents an immunohistochemical comparative study of stellate cells in normal and tumorous pituitary glands in dogs and horses. The study examined the presence and distribution of several proteins and antigens within these cells and found a significant presence of S100 protein subunits. Results also indicated the stellate cells in these animals are more closely related to epithelial than glial cells, a characteristic not seen in humans or mice.
Objective of the Research
- The main aim of the research was to study the presence and distribution of certain proteins and antigens in stellate cells, both in normal and tumorous pituitary glands of dogs and horses. Proteins and antigens, like the S100 protein (alpha and beta subunits), cytokeratin polypeptides, etc., were examined in the research.
Methodology of the Study
- The study was conducted immunochemically on four normal canine pituitary glands, five canine pituitary adenomas, two canine pituitary carcinomas and two equine pituitary adenomas, along with surrounding normal glandular tissue.
- Its focus was on the stellate cells of the pars distalis and pars intermedia of the adenohypophyses (anterior pituitary gland) of both dogs and horses.
Key Findings of the Research
- The stellate cells showed a strong reaction with antibodies against S100 protein subunits alpha and beta.
- In contrast, the stellate cells did not react with antibodies against intermediate filament proteins, neuroendocrine markers, HLA-class II DR antigen or pituitary hormones.
- Other cell types, i.e., polygonal cells and marginal epithelial cells, also expressed both subunits of the S100 protein.
- An interesting observation was that the distribution of alpha and beta subunits in pituitary tumour cells differed between dogs and horses.
Conclusion and Suggestions of the Study
- The study concluded that stellate cells of the adenohypophysis in dogs and horses are more closely related to epithelial than to glial cells, distinct from the case in humans or mice.
- No subpopulation of cells of bone marrow origin was identified among the canine stellate cells, as they lack MHC class II antigen.
- The researchers noted that the presence of S100-immunoreactive cells was more striking in canine and equine tumours compared to human tumours.
Cite This Article
APA
Méndez A, Martín de las Mulas J, Bautista MJ, Chacón F, Millán Y, Fondevila D, Pumarola M.
(1998).
Comparative immunohistochemical study of stellate cells in normal canine and equine adenohypophyses and in pituitary tumours.
J Comp Pathol, 118(1), 29-40.
https://doi.org/10.1016/s0021-9975(98)80025-x Publication
Researcher Affiliations
- Department of Comparative Pathology, Veterinary School, University of Córdoba, Spain.
MeSH Terms
- Adenoma / chemistry
- Adenoma / pathology
- Adenoma / veterinary
- Animals
- Carcinoma / chemistry
- Carcinoma / pathology
- Carcinoma / veterinary
- Dog Diseases / metabolism
- Dog Diseases / pathology
- Dogs
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Immunoenzyme Techniques
- Pituitary Gland, Anterior / cytology
- Pituitary Gland, Anterior / pathology
- Pituitary Neoplasms / chemistry
- Pituitary Neoplasms / pathology
- Pituitary Neoplasms / veterinary
Citations
This article has been cited 2 times.- Acosta M, Filippa V, Mohamed F. Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study. Eur J Histochem 2010 Jan 20;54(1):e1.
- Salinas E, Quintanar JL. Thyroidectomy induces neurofilament expression in adenohypophyses of rats. Endocrine 2001 Aug;15(3):317-22.
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