Comparative mapping of 18 equine type I genes assigned by somatic cell hybrid analysis.
Abstract: Polymerase chain reaction primers designed from horse cDNA sequences and from consensus sequences highly conserved in mammalian species were used to amplify markers for synteny mapping 18 equine type I genes. These markers were used to screen a horse-mouse somatic cell hybrid panel (UCDavis SCH). Fourteen primer sets amplified horse-specific fragments, while restriction enzyme digests of PCR products were used to distinguish the fragments amplified from horse and mouse with four primer sets. Synteny assignments were made based on correlation values between each marker tested and other markers in the UCDavis SCH panel database. The 18 horse genes were assigned to previously established synteny groups. Synteny mapping of two genes previously mapped in the horse by FISH was used to anchor two UCD synteny groups to horse chromosomes. Previous chromosome assignments of three equine loci by FISH were confirmed. Comparative mapping analysis based on published human-horse Zoo-FISH data and the synteny mapping of 14 horse genes confirmed the physical assignment of 12 synteny groups to the respective horse chromosomes and was used to infer the physical location of one synteny group.
Publication Date: 1999-03-02 PubMed ID: 10051323DOI: 10.1007/s003359900985Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the mapping of 18 equine type I genes using somatic cell hybrid analysis. The study further supports and confirms the previous physical assignments of certain gene groups to horse chromosomes.
Overview of the Study
- The study carried out comparative mapping of 18 equine type I genes. For this task, the researchers utilized polymerase chain reaction (PCR) primers created from horse cDNA sequences and consensus sequences common in mammalian species.
- The PCR primers were used to amplify markers for mapping the synteny of the 18 equine type I genes. These markers were then used to examine a horse-mouse somatic cell hybrid panel, or UCDavis SCH.
- Of all the primers, 14 successfully amplified horse-specific fragments. Meanwhile, the other four were distinguished by restriction enzyme digests targeting the PCR products.
Synteny Assignments
- The researchers determined synteny assignments by comparing the correlation values between each tested marker and other markers in the UCDavis SCH panel database.
- The aforementioned 18 horse genes were then assigned to existing synteny groups – groups of genes on a chromosome that are inherited together. This establishes a genetic map for the horse genome.
Comparative Mapping Analysis
- Two genes previously mapped in the horse by Fluorescence in situ hybridization (FISH), were mapped and anchored to horse chromosomes, providing a physical reference for the UCD synteny groups.
- The chromosome assignments of three equine loci previously mapped with FISH were confirmed in this study. This supports the accuracy of the prior mapping via FISH.
- Last, the researchers used Zoo-FISH data in combination with the synteny mapping of 14 horse genes to confirm the physical placement of 12 synteny groups to their appropriate horse chromosomes and infer the physical location of one synteny group.
In summary, the defined objectives of this research were realized. The study successfully mapped 18 equine type I genes and corroborated previous assignment of certain gene groups to horse chromosomes.
Cite This Article
APA
Caetano AR, Pomp D, Murray JD, Bowling AT.
(1999).
Comparative mapping of 18 equine type I genes assigned by somatic cell hybrid analysis.
Mamm Genome, 10(3), 271-276.
https://doi.org/10.1007/s003359900985 Publication
Researcher Affiliations
- University of California-Davis, Veterinary Genetics Laboratory, Davis, California 95616-8744, USA.
MeSH Terms
- Animals
- Base Sequence
- Chromosome Mapping / methods
- Chromosome Mapping / veterinary
- DNA Primers
- DNA, Complementary
- Horses / genetics
- Hybrid Cells
- Polymerase Chain Reaction
Citations
This article has been cited 5 times.- Dall'Olio S, Fontanesi L, Nanni Costa L, Tassinari M, Minieri L, Falaschini A. Analysis of horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types. J Biomed Biotechnol 2010;2010.
- Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals. Mamm Genome 2005 Aug;16(8):631-49.
- Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis. Chromosome Res 2001;9(1):53-9.
- Shiue Y-L, Millon LV, Skow LC, Honeycutt D, Murray JD, Bowling AT. Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes. Chromosome Res 2000;8(1):45-55.
- Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. A comparative gene map of the horse (Equus caballus). Genome Res 1999 Dec;9(12):1239-49.
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