Comparative Proteome Analysis of Serum Uncovers Differential Expression of Proteins in Donkeys (Equus Asinus) With Endometritis Caused by Escherichia Coli.
Abstract: Endometritis is a common disease in donkeys that causes economic losses to donkey farms and the common cause is bacterial infection. Uterine flush fluid proteomics has been used to study protein biomarkers associated with endometritis in mares. As a convenient diagnostic tool, serum proteomics has not been studied yet in equine species with endometritis. This study is aiming to evaluate the serum proteomics in jennies with and without endometritis and identify potential proteins as biomarker for endometritis diagnosis. Nine donkeys recruited into this study were diagnosed of bacterial (Escherichia coli) endometritis and nine healthy jennies were selected as control. Blood samples of each donkey was collected, and serum was separated from each sample. Peptides samples extracted from the serum were analyzed using nano-ultrahigh-performance liquid chromatography-tandem mass spectrometry in data-independent acquisition mode. Protein identification and quantification were performed followed by differential and functional analysis. Of 579 proteins identified in all jennies, 12 proteins were exclusively identified in jennies with endometritis (group E) including myeloperoxidase and Ras-related protein Rab-1B, which might be associated with bacterial infection. There were 11 differentially expressed proteins detected between the two groups of jennies with 4 downregulated proteins and 7 upregulated proteins in jennies with endometritis. Some upregulated proteins along with the GO and KEGG annotation indicated inflammatory response against uterine infection. Characteristic serum proteins identified in jennies with endometritis were associated with inflammation or bacterial infection. These proteins might be potential biomarkers for endometritis diagnosis in jennies.
Copyright © 2023. Published by Elsevier Inc.
Publication Date: 2023-01-07 PubMed ID: 36623579DOI: 10.1016/j.jevs.2023.104221Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research is about identifying potential biomarkers for diagnosing endometritis in donkeys caused by E.coli, using serum proteomics. Results showed certain proteins were differentially expressed in diseased donkeys compared to healthy ones, suggestive of inflammation or bacterial infection.
Methodology
- The study used 18 donkeys; 9 healthy and 9 diagnosed with bacterial (E.coli) endometritis.
- Blood samples were collected from each donkey, from which serum was separated to analyze their proteome.
- The peptides in the serum were examined using nano-ultrahigh-performance liquid chromatography-tandem mass spectrometry in data-independent acquisition mode.
- This method yielded protein identification and quantification, which was then used to conduct differential and functional analysis.
Findings
- 579 proteins were identified across all donkeys. Among these, 12 were found only in the donkeys with endometritis, including myeloperoxidase and Ras-related protein Rab-1B, indicating a potential association with bacterial infection.
- A total of 11 proteins were differentially expressed between the two groups; 4 were downregulated and 7 upregulated in the diseased donkeys.
- These upregulated proteins and their respective GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) annotations suggested an inflammatory response against uterine infection.
- The researchers concluded that these characteristic serum proteins found exclusively in donkeys with endometritis could be potential biomarkers for diagnosing the disease.
Implications
- This study provides a basis for the development of a diagnostic kit based on proteomics to detect endometritis in donkeys.
- It paves the way for further in-depth studies on the mechanism of bacterial endometritis in donkeys and other equine species.
- Diagnosing endometritis at an early stage can help improve treatment success rates, reduce economic losses to farms, and improve overall equine health.
Cite This Article
APA
Li J, Zhao Y, Mi J, Yi Z, Holyoak GR, Wu R, Wang Z, Zhu Y, Zeng S.
(2023).
Comparative Proteome Analysis of Serum Uncovers Differential Expression of Proteins in Donkeys (Equus Asinus) With Endometritis Caused by Escherichia Coli.
J Equine Vet Sci, 122, 104221.
https://doi.org/10.1016/j.jevs.2023.104221 Publication
Researcher Affiliations
- Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China; National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
- Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
- School of Veterinary Science, University of Sydney, Sydney, Australia.
- Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
- College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, USA.
- Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
- College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
- Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing, China.
- National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address: zengsm@cau.edu.cn.
MeSH Terms
- Horses
- Animals
- Female
- Equidae / physiology
- Endometritis / veterinary
- Proteome
- Escherichia coli
- Horse Diseases
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