Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts.
Abstract: Airway fibroblasts have become a critical addition to all facets of structural lung tissue changes such as in human asthma and chronic obstructive pulmonary disease, but little is known about their role in the equine recurrent airway obstruction, a disease that resembles to the human asthma. Since the equine bronchial fibroblasts (EBF) have not been isolated and characterized yet, the use of defined medium was investigated. Results: Primary EBF were cultured on non-collagen coated flasks without serum or in the presence of fetal bovine serum (FBS) or horse serum (HS) or in serum depleted medium. EBF cultured in serum-free basal media and those serum deprived were not able to proliferate and even exhibited considerable cell death. In media containing FBS or HS, proliferation of the cells was reproducible between different primary cultures and cells demonstrated expression of vimentin. Large variations were found in the ability of FBS and HS to support growth and differentiation of EBF in monolayer culture. Indications of growth-promoting actions, increasing passage number as well as maintaining fibroblast morphology were found rather in FBS than in HS. EBF culturing in HS needed longer doubling and confluence time. The protein content of the cell pellets was higher in EBF cultured in medium containing HS than FBS. Alpha-smooth muscle actin seemed to be less expressed in EBF cultured in medium containing FBS than those in HS. Conclusions: In sum, serum addition to basal EBF medium enhanced EBF differentiation into myofibroblasts, and these findings are useful to develop in vitro fibroblast culture models that mimic in vivo physiological processes and to study airway disease mechanisms and remodeling.
Publication Date: 2014-05-26 PubMed ID: 24886635PubMed Central: PMC4040117DOI: 10.1186/1746-6148-10-119Google Scholar: Lookup
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- Comparative Study
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the comparative effects of using fetal bovine serum and horse serum in the growth and development of primary bronchial fibroblasts in horses. The results of this study are beneficial for understanding and studying the mechanisms of airway diseases and the process of remodeling them.
Background and Objective
- The research aimed to examine the effects of using different serums, specifically fetal bovine serum (FBS) and horse serum (HS), on the growth and differentiation of equine bronchial fibroblasts (EBF).
- This study was carried out due to the emerging importance of airway fibroblasts in lung tissue changes in diseases like asthma and chronic obstructive pulmonary disease.
- Although the role of such fibroblasts in equine recurrent airway obstruction—similar to human asthma—was not well known, the investigation into a defined medium for EBF could be of potential value.
Methods and Results
- EBF was cultured in non-collagen coated flasks in the absence of serum, with FBS or HS or in serum-depleted medium.
- EBF that were devoid of serum, or those deprived, suffered from considerable cell death and couldn’t proliferate.
- In the presence of FBS and HS, cell proliferation was evident, with reproducible results across different primary cultures. Additionally, these cells showcased vimentin expression.
- However, differences were observed in the abilities of FBS and HS to promote EBF growth and differentiation, with FBS seeming to support growth-promotion, passage number increment and morphology preservation better than HS.
Detailed Observations and Conclusion
- EBF cultured with horse serum took longer to reach population doubling and confluence times.
- The protein content of EBF was higher when cultured in HS as compared to FBS.
- Alpha-smooth muscle actin, a signifier of fibroblast to myofibroblast transition, was less expressed in cells cultured in FBS as compared to those in HS.
- In conclusion, addition of serum to EBF medium resulted in improved differentiation of EBF into myofibroblasts. This aids in developing effective in vitro fibroblast culture models that resemble in vivo physiological processes and facilitate the understanding of airway disease mechanisms and remodeling.
Cite This Article
APA
Franke J, Abs V, Zizzadoro C, Abraham G.
(2014).
Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts.
BMC Vet Res, 10, 119.
https://doi.org/10.1186/1746-6148-10-119 Publication
Researcher Affiliations
- Institute of Pharmacology, Pharmacy and Toxicology, University of Leipzig, An den Tierkliniken 15, Leipzig 04103, Germany. gabraham@rz.uni-leipzig.de.
MeSH Terms
- Actins / genetics
- Actins / metabolism
- Animals
- Bronchi / cytology
- Cattle
- Cell Culture Techniques
- Cell Survival
- Culture Media / chemistry
- Fibroblasts / drug effects
- Fibroblasts / physiology
- Gene Expression Regulation / drug effects
- Horses
- Serum
References
This article includes 37 references
Citations
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- Petrova V, Vachkova E. Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses.. Vet Sci 2023 May 12;10(5).
- Xie D, Deng T, Zhai Z, Sun T, Xu Y. The cellular model for Alzheimer's disease research: PC12 cells.. Front Mol Neurosci 2022;15:1016559.
- Pilgrim CR, McCahill KA, Rops JG, Dufour JM, Russell KA, Koch TG. A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine.. Front Vet Sci 2022;9:859025.
- Rampin A, Skoufos I, Raghunath M, Tzora A, Diakakis N, Prassinos N, Zeugolis DI. Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.. Cells 2022 May 5;11(9).
- Ribeiro MC, Rivera-Arbeláez JM, Cofiño-Fabres C, Schwach V, Slaats RH, Ten Den SA, Vermeul K, van den Berg A, Pérez-Pomares JM, Segerink LI, Guadix JA, Passier R. A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues.. J Pers Med 2022 Feb 4;12(2).
- Jeong Y, Choi WY, Park A, Lee YJ, Lee Y, Park GH, Lee SJ, Lee WK, Ryu YK, Kang DH. Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.. Sci Rep 2021 Mar 1;11(1):4906.
- Samareh Salavati Pour M, Vahidi R, Lashkari M, Derakhshani A, Ameri Z, Farsinejad A. Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells.. Cytotechnology 2020 Aug;72(4):551-567.
- Ou Q, Abouleisa RRE, Tang XL, Juhardeen HR, Meki MH, Miller JM, Giridharan G, El-Baz A, Bolli R, Mohamed TMA. Slicing and Culturing Pig Hearts under Physiological Conditions.. J Vis Exp 2020 Mar 20;(157).
- Abs V, Bonicelli J, Kacza J, Zizzadoro C, Abraham G. Equine bronchial fibroblasts enhance proliferation and differentiation of primary equine bronchial epithelial cells co-cultured under air-liquid interface.. PLoS One 2019;14(11):e0225025.
- Ou Q, Jacobson Z, Abouleisa RRE, Tang XL, Hindi SM, Kumar A, Ivey KN, Giridharan G, El-Baz A, Brittian K, Rood B, Lin YH, Watson SA, Perbellini F, McKinsey TA, Hill BG, Jones SP, Terracciano CM, Bolli R, Mohamed TMA. Physiological Biomimetic Culture System for Pig and Human Heart Slices.. Circ Res 2019 Aug 30;125(6):628-642.
- Duan J, Navarro-Dorado J, Clark JH, Kinnear NP, Meinke P, Schirmer EC, Evans AM. The cell-wide web coordinates cellular processes by directing site-specific Ca(2+) flux across cytoplasmic nanocourses.. Nat Commun 2019 May 24;10(1):2299.
- Kim SH, Jeong JY, Park HJ, Moon BM, Park YR, Lee OJ, Sultan MT, Kim DK, Park HS, Lee JH, Park CH. Application of a Collagen Patch Derived from Duck Feet in Acute Tympanic Membrane Perforation.. Tissue Eng Regen Med 2017 Jun;14(3):233-241.
- Eydt C, Geburek F, Schröck C, Hambruch N, Rohn K, Pfarrer C, Staszyk C. Sternal bone marrow derived equine multipotent mesenchymal stromal cells (MSCs): investigations considering the sampling site and the use of different culture media.. Vet Med Sci 2016 Aug;2(3):200-210.
- Nims RW, Harbell JW. Best practices for the use and evaluation of animal serum as a component of cell culture medium.. In Vitro Cell Dev Biol Anim 2017 Sep;53(8):682-690.
- Fang CY, Wu CC, Fang CL, Chen WY, Chen CL. Long-term growth comparison studies of FBS and FBS alternatives in six head and neck cell lines.. PLoS One 2017;12(6):e0178960.
- Ziegler A, Everett H, Hamza E, Garbani M, Gerber V, Marti E, Steinbach F. Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.. BMC Vet Res 2016 Nov 15;12(1):254.
- Poosala P, Ichinose H, Kitaoka T. Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion.. Int J Mol Sci 2016 May 6;17(5).
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