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BMC veterinary research2014; 10; 119; doi: 10.1186/1746-6148-10-119

Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts.

Abstract: Airway fibroblasts have become a critical addition to all facets of structural lung tissue changes such as in human asthma and chronic obstructive pulmonary disease, but little is known about their role in the equine recurrent airway obstruction, a disease that resembles to the human asthma. Since the equine bronchial fibroblasts (EBF) have not been isolated and characterized yet, the use of defined medium was investigated. Results: Primary EBF were cultured on non-collagen coated flasks without serum or in the presence of fetal bovine serum (FBS) or horse serum (HS) or in serum depleted medium. EBF cultured in serum-free basal media and those serum deprived were not able to proliferate and even exhibited considerable cell death. In media containing FBS or HS, proliferation of the cells was reproducible between different primary cultures and cells demonstrated expression of vimentin. Large variations were found in the ability of FBS and HS to support growth and differentiation of EBF in monolayer culture. Indications of growth-promoting actions, increasing passage number as well as maintaining fibroblast morphology were found rather in FBS than in HS. EBF culturing in HS needed longer doubling and confluence time. The protein content of the cell pellets was higher in EBF cultured in medium containing HS than FBS. Alpha-smooth muscle actin seemed to be less expressed in EBF cultured in medium containing FBS than those in HS. Conclusions: In sum, serum addition to basal EBF medium enhanced EBF differentiation into myofibroblasts, and these findings are useful to develop in vitro fibroblast culture models that mimic in vivo physiological processes and to study airway disease mechanisms and remodeling.
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Publication Date: 2014-05-26 PubMed ID: 24886635PubMed Central: PMC4040117DOI: 10.1186/1746-6148-10-119Google Scholar: Lookup
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  • Comparative Study
  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the comparative effects of using fetal bovine serum and horse serum in the growth and development of primary bronchial fibroblasts in horses. The results of this study are beneficial for understanding and studying the mechanisms of airway diseases and the process of remodeling them.

Background and Objective

  • The research aimed to examine the effects of using different serums, specifically fetal bovine serum (FBS) and horse serum (HS), on the growth and differentiation of equine bronchial fibroblasts (EBF).
  • This study was carried out due to the emerging importance of airway fibroblasts in lung tissue changes in diseases like asthma and chronic obstructive pulmonary disease.
  • Although the role of such fibroblasts in equine recurrent airway obstruction—similar to human asthma—was not well known, the investigation into a defined medium for EBF could be of potential value.

Methods and Results

  • EBF was cultured in non-collagen coated flasks in the absence of serum, with FBS or HS or in serum-depleted medium.
  • EBF that were devoid of serum, or those deprived, suffered from considerable cell death and couldn’t proliferate.
  • In the presence of FBS and HS, cell proliferation was evident, with reproducible results across different primary cultures. Additionally, these cells showcased vimentin expression.
  • However, differences were observed in the abilities of FBS and HS to promote EBF growth and differentiation, with FBS seeming to support growth-promotion, passage number increment and morphology preservation better than HS.

Detailed Observations and Conclusion

  • EBF cultured with horse serum took longer to reach population doubling and confluence times.
  • The protein content of EBF was higher when cultured in HS as compared to FBS.
  • Alpha-smooth muscle actin, a signifier of fibroblast to myofibroblast transition, was less expressed in cells cultured in FBS as compared to those in HS.
  • In conclusion, addition of serum to EBF medium resulted in improved differentiation of EBF into myofibroblasts. This aids in developing effective in vitro fibroblast culture models that resemble in vivo physiological processes and facilitate the understanding of airway disease mechanisms and remodeling.

Cite This Article

APA
Franke J, Abs V, Zizzadoro C, Abraham G. (2014). Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts. BMC Vet Res, 10, 119. https://doi.org/10.1186/1746-6148-10-119

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 10
Pages: 119

Researcher Affiliations

Franke, Jana
    Abs, Vanessa
      Zizzadoro, Claudia
        Abraham, Getu
        • Institute of Pharmacology, Pharmacy and Toxicology, University of Leipzig, An den Tierkliniken 15, Leipzig 04103, Germany. gabraham@rz.uni-leipzig.de.

        MeSH Terms

        • Actins / genetics
        • Actins / metabolism
        • Animals
        • Bronchi / cytology
        • Cattle
        • Cell Culture Techniques
        • Cell Survival
        • Culture Media / chemistry
        • Fibroblasts / drug effects
        • Fibroblasts / physiology
        • Gene Expression Regulation / drug effects
        • Horses
        • Serum

        References

        This article includes 37 references

        Citations

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