Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse.
Abstract: Insulin-like growth factor-1 (IGF-1) plays several important physiological roles, and IGF-related pathways have been implicated in developmental osteochondral disease and endocrinopathic laminitis. This factor is also a downstream marker of growth hormone activity and its peptide mimetics. Unfortunately, previously used assays for measuring equine IGF-1 (radioimmunoassays and ELISAs) are no longer commercially available, and many of the kits on the market give poor results when used on horse samples. The aim of the present study was to compare three different ELISA assays (two human and one horse-specific). Plasma samples from six Standardbreds, six ponies and six Andalusians were used. The human IGF-1 ELISA kit from Immunodiagnostic Systems (IDS) proved to be the most accurate and precise of the three kits; the other two assays gave apparently much lower concentrations, with poor recovery of spiked recombinant human IGF-1 and unacceptably poor intra-assay coefficients of variation (CV). The IDS assay gave an intra-assay CV of 3.59 % and inter-assay CV of 7.31%. Mean percentage recovery of spiked IGF-1 was 88.82%, and linearity and dilutional parallelism were satisfied. The IGF-1 plasma concentrations were 123.21 ±8.24 ng/mL for Standardbreds, 124.95 ±3.69 ng/mL for Andalusians and 174.26 ±1.94 ng/mL for ponies. Therefore of the three assays assessed, the IGF-1 ELISA manufactured by IDS was the most suitable for use with equine plasma samples and may have many useful applications in several different research areas. However, caution should be used when comparing equine studies where different analytical techniques and assays may have been used to measure this growth factor.
Publication Date: 2017-03-31 PubMed ID: 28540255PubMed Central: PMC5443403DOI: 10.4314/ovj.v7i1.12Google Scholar: Lookup
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- Journal Article
Summary
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This study examined three ELISA assays for accurately measuring insulin-like growth factor-1 (IGF-1) in horses. The researchers found the human IGF-1 ELISA kit from Immunodiagnostic Systems (IDS) was the most accurate and consistent of the kits tested.
Objective of the Research
- The purpose of this study was to evaluate three different ELISA assays (two designed for humans and one for horses) for their ability to measure insulin-like growth factor-1 (IGF-1) in horse plasma.
The Importance of IGF-1
- IGF-1 has several important physiological roles in the body, including as an indicator of growth hormone activity and its peptide mimetics. It is especially relevant in the context of certain equine diseases such as developmental osteochondral disease and endocrinopathic laminitis.
The Problem with Existing Assays
- The researchers pointed out that many of the existing assays for equine IGF-1 measurement are either no longer commercially available or give inaccurate results when used with horse samples. This required the development and validation of new assays.
Comparative Analysis of Three ELISA Assays
- The researchers compared three different ELISA assays using plasma samples from six Standardbreds, six ponies, and six Andalusians.
- The human IGF-1 ELISA kit from Immunodiagnostic Systems (IDS) demonstrated the highest levels of accuracy and precision. The other two assays showed low concentrations and poor recovery of IGF-1, and weren’t consistent in their results, making them less reliable.
Conclusion
- The researchers concluded that, based on their comparative analysis, the IGF-1 ELISA kit produced by IDS was the most suitable for measuring IGF-1 in equine plasma samples.
- However, they also noted caution should be exercised when comparing different studies on equine IGF-1, as various techniques and assays may have been used, potentially leading to inconsistencies.
Cite This Article
APA
Baskerville CL, Bamford NJ, Harris PA, Bailey SR.
(2017).
Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse.
Open Vet J, 7(1), 75-80.
https://doi.org/10.4314/ovj.v7i1.12 Publication
Researcher Affiliations
- Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia.
- Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia.
- Equine Studies Group, WALTHAM Centre for Pet Nutrition, Melton Mowbray, Leicestershire, UK.
- Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia.
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Citations
This article has been cited 1 times.- Koch DW, Schnabel LV, Ellis IM, Bates RE, Berglund AK. TGF-β2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing.. Stem Cell Res Ther 2022 Sep 16;13(1):477.
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