Comparison of 4 agar gel immunodiffusion kits for serologic detection of equine infectious anemia virus antibodies.
Abstract: Using 85 sera collected from horses that had been experimentally infected with equine infectious anemia virus (EIAV) and 200 field sera collected from racehorses in Japan, we compared 4 agar gel immunodiffusion (AGID) kits for serologic detection of EIAV antibodies from Idexx, VMRD, IDvet, and the National Engineering Research Center of Veterinary Biologics, China (NECVB). The positive control lines were sufficiently clear in all kits for evaluation to be made, with slight differences in sharpness: NECVB was the sharpest, followed by VMRD, IDvet, and Idexx. The test results for all 285 samples agreed among the 4 kits, with 62 positives and 223 negatives. The sensitivities and specificities of VMRD, IDvet, and NECVB compared with the Idexx kit were 100%, and the kappa coefficient values between the kits were 1.0 for all combinations. We concluded that the testing capacity of these 4 kits was virtually identical.
Publication Date: 2023-05-02 PubMed ID: 37129383PubMed Central: PMC10331386DOI: 10.1177/10406387231171567Google Scholar: Lookup
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- Journal Article
Summary
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The research compared four testing kits used to detect equine infectious anemia virus (EIAV) antibodies in horses and found that the performance of all four kits was virtually identical.
Objective and Methodology
- The main objective of the research was to compare the performance of four separate agar gel immunodiffusion (AGID) kits. These kits are designed to detect the presence of equine infectious anemia virus (EIAV) antibodies in horse sera.
- The kits compared were from Idexx, VMRD, IDvet, and the National Engineering Research Center of Veterinary Biologics, China (NECVB).
- The researchers used a total of 285 serum samples, 85 of which were from horses experimentally infected with EIAV, and the remaining 200 were collected from racehorses in Japan.
Findings
- All the kits produced clear positive control lines, which varied slightly in sharpness. The sharpest line was produced by the NECVB kit, followed by VMRD, IDvet, and Idexx.
- The test results from all 285 samples were consistent across all four kits. There were 62 positive and 223 negative results.
- The sensitivity and specificity of VMRD, IDvet, and NECVB kits were all found to be 100% compared to the Idexx kit.
- The kappa coefficient values, which measure the agreement between the kits, were 1.0 for all combinations, indicating perfect agreement.
Conclusion
- The researchers concluded that the testing capacity of the four kits was virtually identical.
- This means that all four kits are identically effective in detecting equine infectious anemia virus (EIAV) antibodies in horse sera.
Cite This Article
APA
Bannai H, Kambayashi Y, Nemoto M, Yamanaka T, Tsujimura K.
(2023).
Comparison of 4 agar gel immunodiffusion kits for serologic detection of equine infectious anemia virus antibodies.
J Vet Diagn Invest, 35(4), 430-432.
https://doi.org/10.1177/10406387231171567 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
MeSH Terms
- Animals
- Horses
- Infectious Anemia Virus, Equine
- Equine Infectious Anemia / diagnosis
- Agar
- Immunodiffusion / veterinary
- Immunodiffusion / methods
- Antibodies, Viral
- Enzyme-Linked Immunosorbent Assay / veterinary
- Horse Diseases
Conflict of Interest Statement
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
References
This article includes 10 references
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Citations
This article has been cited 3 times.- Liang H, Zhou B, Hu Z, Chu X, Wang X, Du C, Wang X. Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus. Microorganisms 2025 Jun 27;13(7).
- Ostuni A, Frontoso R, Crudele MA, Barca L, Amati M, Boni R, De Vendel J, Raimondi P, Bavoso A. Comparative Evaluation of a Multistrain Indirect ELISA Targeting Anti- p26 and gp45 Antibodies for EIAV Detection. Pathogens 2025 Jun 8;14(6).
- Domínguez-Odio A, González LIC, Alfonso DM, Guevara-Hernández F, La O Arias MA, Zayas MP, Ríos MÁB. Serodiagnosis of equine infectious anemia by indirect ELISA based on a novel synthetic peptide derived from gp45 glycoprotein. Vet Res Commun 2025 Apr 22;49(3):174.
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