Comparison of autologous bone marrow and adipose tissue derived mesenchymal stem cells, and platelet rich plasma, for treating surgically induced lesions of the equine superficial digital flexor tendon.
Abstract: Several therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons). The remaining six tendons received lactated Ringer's solution as control. Serial ultrasound assessment was performed prior to treatment and at 2, 6, 10, 20 and 45 weeks post-treatment. At 45 weeks, histopathology and gene expression analyses were performed. At week 6, the ultrasound echogenicity score in tendons treated with BM-MSCs suggested earlier improvement, whilst all treatment groups reached the same level at week 10, which was superior to the control group. Collagen orientation scores on histological examination suggested a better outcome in treated tendons. Gene expression was indicative of better tissue regeneration after all treatments, especially for BM-MSCs, as suggested by upregulation of collagen type I, decorin, tenascin and matrix metalloproteinase III mRNA. Considering all findings, a clear beneficial effect was elicited by all treatments compared with the control group. Although differences between treatments were relatively small, BM-MSCs resulted in a better outcome than PRP and AT-MSCs.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication Date: 2017-05-02 PubMed ID: 28697880DOI: 10.1016/j.tvjl.2017.04.005Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research is about assessing the effectiveness of three different treatments for injuries to the superficial digital flexor tendon in horses. The best results were seen with bone marrow-derived mesenchymal stem cells, but all treatments were better than the control.
Background and Objectives
- The authors of this study wanted to understand the mechanism of healing enhanced by regenerative therapies on equine tendinopathies. Despite numerous therapies being investigated, consistent satisfactory long-term results haven’t been achieved.
- The three therapies in testing were autologous bone marrow and adipose tissue-derived mesenchymal stem cells (BM-MSCs and AT-MSCs respectively), and platelet-rich plasma (PRP).
- The goal was to compare the respective effects of these treatments on surgically induced lesions in the superficial digital flexor tendon (SDFT) of horses.
Methods
- The researchers created lesions in both SDFTs of the forelimbs of 12 horses.
- Six tendons were treated with BM-MSCs, six with AT-MSCs, and six with PRP. A control group of the remaining six tendons were treated with a lactated Ringer’s solution.
- They then carried out an ultrasound assessment prior to the treatment and at the intervals of 2, 6, 10, 20, and 45 weeks post-treatment.
- At the 45-week mark, the scientists performed histopathology and gene expression analyses.
Results
- At week 6, tendons treated with BM-MSCs showed signs of earlier improvement in the ultrasound echogenicity score. However, by week 10, all treatment groups had the same level which was superior to the control group.
- On histological examination, collagen orientation scores hinted at a better outcome in treated tendons than in the control group.
- Gene expression analyses pointed to improved tissue regeneration in all treatment groups, especially the BM-MSC group. This was suggested by the upregulation of collagen type I, decorin, tenascin, and matrix metalloproteinase III mRNA.
Conclusions
- The findings of the study demonstrated a clear beneficial effect from all treatments compared to the control group.
- Of the three treatments, the outcomes from the BM-MSCs were slightly superior to the outcomes from PRP and AT-MSCs, indicating it as the more promising candidate for treating equine tendinopathies.
Cite This Article
APA
Romero A, Barrachina L, Ranera B, Remacha AR, Moreno B, de Blas I, Sanz A, Vázquez FJ, Vitoria A, Junquera C, Zaragoza P, Rodellar C.
(2017).
Comparison of autologous bone marrow and adipose tissue derived mesenchymal stem cells, and platelet rich plasma, for treating surgically induced lesions of the equine superficial digital flexor tendon.
Vet J, 224, 76-84.
https://doi.org/10.1016/j.tvjl.2017.04.005 Publication
Researcher Affiliations
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain.
- Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain.
- Laboratorio de Genética Bioquímica (LAGENBIO), Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, 50013 Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), 50013 Zaragoza, Spain. Electronic address: rodellar@unizar.es.
MeSH Terms
- Adipose Tissue / cytology
- Animals
- Autografts
- Bone Marrow Transplantation / veterinary
- Horse Diseases / surgery
- Horse Diseases / therapy
- Horses
- Intraoperative Complications / therapy
- Intraoperative Complications / veterinary
- Mesenchymal Stem Cell Transplantation / veterinary
- Platelet-Rich Plasma
- Tendinopathy / therapy
- Tendinopathy / veterinary
- Tendon Injuries / etiology
- Tendon Injuries / therapy
- Tendon Injuries / veterinary
- Tendons / diagnostic imaging
- Tendons / pathology
- Tendons / surgery
- Ultrasonography / veterinary
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