Comparison of Enterprise Point-of-Care and Nova Biomedical Critical Care Xpress analyzers for determination of arterial pH, blood gas, and electrolyte values in canine and equine blood.
Abstract: Point-of-care analyzers can provide a rapid turnaround time for critical blood test results. Agreement between the Enterprise Point-of-Care (EPOC) and bench-top laboratory analyzers is important to determine the clinical reliability of the EPOC. Objective: The aim of the study was (1) to evaluate the precision (repeatability) of blood gas values measured by the EPOC and (2) to determine the level of agreement between the EPOC and Nova Critical Care Express (Nova CCX) for the assessment of arterial pH, blood gases, and electrolyte variables in canine and equine blood. Methods: Arterial blood samples from dogs were analyzed on the EPOC and Nova CCX analyzers to determine precision and agreement of pH, PaCO , PaO , and HCT. The same analytes plus Na , K , and Cl were analyzed for agreement using equine blood. Statistical analyses included assessment of precision using the coefficient of variation (CV%), and agreement using the Deming regression, Pearson correlation, and Bland-Altman plots. Results: Both analyzers provided precise results of pH, PaCO , PaO and HCT, meeting CV% quality requirement values. In both species, Deming regression results were acceptable and correlation values were above 0.93 for arterial pH and blood gases, but lower for sodium and chloride. Bland-Altman plots demonstrated varying degrees of bias, but good agreement between the 2 analyzers was seen when arterial blood gases and electrolytes were measured, except for PaCO and Cl CONCLUSION: The EPOC analyzer provides consistent, reliable results for canine arterial blood gas values and for equine arterial blood gas and electrolyte values. Cl results could be acceptable with the application of a correction factor, but the PaCO results were more variable.
© 2018 American Society for Veterinary Clinical Pathology.
Publication Date: 2018-07-09 PubMed ID: 29989207DOI: 10.1111/vcp.12635Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article compares the performance of the Enterprise Point-of-Care (EPOC) and Nova Critical Care Express (Nova CCX) blood analyzers, focusing on precision and agreement between these two devices, using canine and equine blood samples.
Objective of the Research
- The objective of the study is twofold. Firstly, it seeks to establish the precision or repeatability of the EPOC in measuring blood gas values. The second objective then focuses on comparing the EPOC with the Nova CCX, specifically in terms of assessing arterial pH, blood gases, and electrolyte variables in canine and equine blood.
Methodology
- To achieve these objectives, the researchers analyzed arterial blood samples from dogs on both the EPOC and Nova CCX analyzers. The aim was to determine the precision and alignment of several variables; specifically, of pH, PaCO, PaO, and HCT.
- Furthermore, through analyzing equine blood, additional analytes such as Na, K, and Cl, were observed for comparison.
- Statistical analyses were conducted with a focus on precision using the coefficient of variation (CV%) and agreement, which was evaluated utilizing Deming regression, Pearson correlation, and Bland-Altman plots.
Results of the Study
- Results indicated that both analyzers offer precise results for pH, PaCO, PaO and HCT, aligning with CV% quality requirement values.
- Broadly, the results of arsenic pH and blood gases in both species showed acceptable Deming regression and correlation values, the latter being above 0.93. Sodium and chloride, however, showed lower correlation.
- Bland-Altman plots exhibited various degrees of bias, but overall substantial agreement between the two analyzers in terms of measuring arterial blood gases and electrolytes was established, with the exception of PaCO and Cl.
Conclusion of the Research
- The research concluded that, overall, the EPOC analyzer offers consistent and reliable results for canine arterial blood gas values and for equine arterial blood gas and electrolyte values.
- For chloride (Cl) results, the researchers suggested that acceptable output could be achieved by applying a correction factor; however, PaCO results were found to be more variable.
Cite This Article
APA
Elmeshreghi TN, Grubb TL, Greene SA, Ragle CA, Wardrop JA.
(2018).
Comparison of Enterprise Point-of-Care and Nova Biomedical Critical Care Xpress analyzers for determination of arterial pH, blood gas, and electrolyte values in canine and equine blood.
Vet Clin Pathol, 47(3), 415-424.
https://doi.org/10.1111/vcp.12635 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
MeSH Terms
- Animals
- Autoanalysis / instrumentation
- Autoanalysis / methods
- Autoanalysis / veterinary
- Blood Gas Analysis / instrumentation
- Blood Gas Analysis / methods
- Blood Gas Analysis / veterinary
- Blood Specimen Collection / veterinary
- Dogs / blood
- Electrolytes / blood
- Horses / blood
- Hydrogen-Ion Concentration
- Point-of-Care Systems
- Reproducibility of Results
Grant Funding
- Washington State University
Citations
This article has been cited 5 times.- Sandersen C, Dmitrovic P, Dupont J, Cesarini C, Guyot H, Serteyn D, Kirsch K. Analytical Performance Evaluation of the New GEM(®) Premier™ 5000 in Comparison to the Epoc(®) Blood Gas Analyzer in Horses.. Vet Sci 2023 Feb 3;10(2).
- Coenen MC, Gille L, Eppe J, Casalta H, Bayrou C, Dubreucq P, Frisée V, Moula N, Evrard J, Martinelle L, Sartelet A, Bossaert P, Djebala S. Blood Inflammatory, Hydro-Electrolytes and Acid-Base Changes in Belgian Blue Cows Developing Parietal Fibrinous Peritonitis or Generalised Peritonitis after Caesarean Section.. Vet Sci 2022 Mar 14;9(3).
- Nawrocki J, Furian M, Buergin A, Mayer L, Schneider S, Mademilov M, Bloch MS, Sooronbaev TM, Ulrich S, Bloch KE. Validation of a Portable Blood Gas Analyzer for Use in Challenging Field Conditions at High Altitude.. Front Physiol 2020;11:600551.
- Burchell RK, Gal A, Friedlein R, Leisewitz AL. Role of electrolyte abnormalities and unmeasured anions in the metabolic acid-base abnormalities in dogs with parvoviral enteritis.. J Vet Intern Med 2020 Mar;34(2):857-866.
- Kirsch K, Detilleux J, Serteyn D, Sandersen C. Comparison of two portable clinical analyzers to one stationary analyzer for the determination of blood gas partial pressures and blood electrolyte concentrations in horses.. PLoS One 2019;14(2):e0211104.
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