Comparison of eosin-thiazin and Papanicolaou-Shorr staining for endometrial cytologies of broodmares. Technical Note.
Abstract: Exfoliative endometrial cytology is an easy and valuable diagnostic tool for the detection of inflammatory processes of the uterus that correlates well to culture results. The practical use of this procedure is limited due to the time-consuming Papanicolaou-Shorr staining technique. In this study the suitability of the rapid eosin-thiazin staining for endometrial smears was investigated. Methods: Sample collection was carried out with a guarded culture swab (Knudsen-catheter) in 27 broodmares during routine gynaecological examination. Two smears were prepared from each collection. One was stained according to Papanicolaou-Shorr and the second using the eosin-thiazin method (Hemacolor®, Merck). Specimens with more than 0.5% neutrophil granulocytes were classified as positive for endometritis. The presentability of polymorphonuclear neutrophils (PMN), proportion of positive/negative samples, as well as the PMN contents were compared between the two staining methods. Results: PMN were easily identifiable in both specimens. In Papanicolaou-Shorr stained smears 10 samples showed > 0.5% neutrophil granulocytes (37%), whereas in the eosin-thiazin staining 12 samples were positive (44%). Thus results corresponded in 25 mares (95%). Conclusions: Eosin-thiazin staining is a suitable staining method for endometrial smears of broodmares, which surpassed Papanicolaou-Shorr method in two cases. Conclusions: The use of eosin-thiazin staining provides a considerable gain of time and renders endometrial cytology more attractive for routine stud farm practice.
Publication Date: 2011-12-15 PubMed ID: 22167080
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- Comparative Study
- Journal Article
Summary
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This research study evaluated and compared the effectiveness of using eosin-thiazin staining versus Papanicolaou-Shorr staining for examining endometrial cytologies in broodmares. The findings suggested that eosin-thiazin staining could be a more efficient method, saving time without compromising the accuracy of the diagnosis.
Methodology
- The study involved 27 broodmares undergoing routine gynaecological examinations.
- A guarded culture swab, known as a Knudsen-catheter, was used to collect samples.
- Two smears were prepared from each collected sample. One was stained using the traditional Papanicolaou-Shorr method and the other with the eosin-thiazin method using Hemacolor.
- The specimines were considered positive for endometritis, an inflammation of the uterus, if they contained more than 0.5% neutrophil granulocytes.
- The study then compared the visibility of polymorphonuclear neutrophils (PMN), the proportion of positive/negative samples, and the PMN contents between the two staining methods.
Results
- In both types of stained smears, PMN were easily identifiable, suggesting both methods are effective for diagnosing endometritis.
- With the Papanicolaou-Shorr staining, 10 out of 27 samples were positive for endometritis (more than 0.5% neutrophil granulocytes), whereas with the eosin-thiazin staining, 12 samples were positive.
- The results from both types of staining corresponded in 25 out of the 27 mares, demonstrating a 95% consistency rate.
- The eosin-thiazin method surpassed the Papanicolaou-Shorr method in two instances, indicating a potential for improved accuracy.
Conclusion
- The results support the use of eosin-thiazin staining as a suitable method for staining endometrial smears in broodmares. This provides a significant time-saving advantage while maintaining accuracy in detecting endometritis.
- Given these benefits, the use of eosin-thiazin staining could make endometrial cytology more appealing for routine stud farm practice.
Cite This Article
APA
Walter J, Klein C, Wehrend A.
(2011).
Comparison of eosin-thiazin and Papanicolaou-Shorr staining for endometrial cytologies of broodmares. Technical Note.
Tierarztl Prax Ausg G Grosstiere Nutztiere, 39(6), 358-362.
Publication
Researcher Affiliations
- Klinik für Fortpflanzungsmedizin, Vetsuisse Fakultät Universität Zürich, Winterthurerstrasse 260, 8057 Zürich, Switzerland. jwalter@vetclinics.uzh.ch
MeSH Terms
- Animals
- Endometritis / pathology
- Endometritis / veterinary
- Endometrium / pathology
- Eosine Yellowish-(YS)
- Female
- Fluorescent Dyes
- Horse Diseases / pathology
- Horses
- Organic Chemicals
- Papanicolaou Test
- Staining and Labeling / methods
- Staining and Labeling / veterinary
- Vaginal Smears / instrumentation
- Vaginal Smears / veterinary
Citations
This article has been cited 3 times.- Lim CJ, Lee SY, Ryu PD. Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats. Evid Based Complement Alternat Med 2015;2015:751937.
- Lim CJ, Yoo JH, Kim Y, Lee SY, Ryu PD. Gross morphological features of the organ surface primo-vascular system revealed by hemacolor staining. Evid Based Complement Alternat Med 2013;2013:350815.
- Salazar Moya J, Rojas-Zumaran V, Vegas C, Salafia A, Contreras-Pulache H. Use of grape-based stain (Vinatela) on cervical cytology: A Peruvian validation study. Cytojournal 2023;20:30.
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