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Comparison of hematologic values and transforming growth factor-beta and insulin-like growth factor concentrations in platelet concentrates obtained by use of buffy coat and apheresis methods from equine blood.
Abstract: To evaluate the buffy coat and apheresis methods for preparation of platelet concentrates from equine blood by comparing platelet and growth factor concentrations. Methods: 15 mature mixed-breed geldings. Methods: Whole blood samples were collected and processed by use of a buffy coat or apheresis method to obtain platelet poor and platelet concentrated fractions. The PCV, WBC count, and platelet count were compared among whole blood samples, platelet poor fractions, concentrates obtained by use of the apheresis method (ie, apheresis platelet concentrates), and concentrates obtained by use of the buffy coat method (ie, buffy coat platelet concentrates). Concentrations of transforming growth factor-beta (ie,TGF-beta1 andTGF-beta2) and insulin-like growth factor were compared between buffy coat and apheresis platelet concentrates. Results: Platelet concentrations were 8.9-fold and 5.2-fold greater in buffy coat and apheresis platelet concentrates, respectively, compared with whole blood. Platelet concentrations were 13.1-fold greater in filtered apheresis platelet concentrates, compared with whole blood. TGF-beta1 concentrations were 2.8-fold and 3.1-fold greater in buffy coat and apheresis platelet concentrates, respectively, and TGF-beta1 concentrations were 10.5-fold greater in filtered apheresis platelet concentrates, compared with whole blood. TGF-beta2 concentrations were 3.6-fold greater in apheresis platelet concentrates, compared with whole blood. Platelet concentrations correlated with growth factor concentrations across all blood and platelet fractions. White blood cell counts had a significant positive correlation with TGF-beta1 concentration in buffy coat platelet concentrates. Conclusions: Platelets and TGF-beta1 can be concentrated reliably from equine blood by use of buffy coat or apheresis methods, without modification of the protocols used for humans.
Publication Date: 2004-07-30 PubMed ID: 15281650DOI: 10.2460/ajvr.2004.65.924Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research paper is examining the feasibility and effectiveness of two methods for extracting platelet concentrates from equine blood: the buffy coat and apheresis methods. It discovers that both methods concentrate platelets and transforming growth factor-beta effectively from horse blood.
Methods Used in the Research
The study featured several important methods:
- The experiment involved 15 mature mixed-breed geldings as subjects.
- Blood samples were taken and processed with either the buffy coat or apheresis process to obtain different platelet fractions.
- The researchers compared the packed cell volume (PCV), white blood cell (WBC) count, and platelet count among whole blood samples, platelet-poor fractions, apheresis platelet concentrates, and buffy coat platelet concentrates.
- Additionally, the levels of insulin-like growth factor and different types of transforming growth factor-beta (TGF-beta1 and TGF-beta2) were compared between the buffy coat method and the apheresis method.
Key Results
The primary findings of the paper were:
- The platelet concentrations were significantly higher in the buffy coat (8.9-fold) and apheresis (5.2-fold) platelet concentrates compared to the whole blood samples.
- Filtered apheresis platelet concentrates yielded a remarkable 13.1-fold more platelets compared with whole blood.
- TGF-beta1 concentrations were significantly more (2.8-fold for buffy coat and 3.1-fold for apheresis) and reached up to 10.5-fold in filtered apheresis platelet concentrates.
- TGF-beta2 concentrations were 3.6-fold greater in apheresis platelet concentrates compared to whole blood.
- A strong correlation was found between platelet concentrations and growth factor concentrations across all blood and platelet fractions.
- A significant positive correlation was observed between white blood cell counts and TGF-beta1 concentration in buffy coat platelet concentrates.
Conclusions Drawn
Based on their findings, the researchers concluded:
- Both the buffy coat and apheresis methods can reliably concentrate platelets and TGF-beta1 from equine blood.
- There was no need to modify the protocols used for humans when applying these methods to equine blood.
Cite This Article
APA
Sutter WW, Kaneps AJ, Bertone AL.
(2004).
Comparison of hematologic values and transforming growth factor-beta and insulin-like growth factor concentrations in platelet concentrates obtained by use of buffy coat and apheresis methods from equine blood.
Am J Vet Res, 65(7), 924-930.
https://doi.org/10.2460/ajvr.2004.65.924 Publication
Researcher Affiliations
- Department of Clinical Sciences, Comparative Orthopedic Molecular Medicine Research Laboratories, The Ohio State University, Columbus, OH 43210, USA.
MeSH Terms
- Analysis of Variance
- Animals
- Blood Platelets / chemistry
- Blood Platelets / cytology
- Hemofiltration / instrumentation
- Hemofiltration / methods
- Horses / blood
- Leukocyte Count / veterinary
- Platelet Count / veterinary
- Plateletpheresis / methods
- Somatomedins / metabolism
- Transforming Growth Factor beta / blood
Citations
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