Comparison of hematologic values and transforming growth factor-beta and insulin-like growth factor concentrations in platelet concentrates obtained by use of buffy coat and apheresis methods from equine blood.
Abstract: To evaluate the buffy coat and apheresis methods for preparation of platelet concentrates from equine blood by comparing platelet and growth factor concentrations. Methods: 15 mature mixed-breed geldings. Methods: Whole blood samples were collected and processed by use of a buffy coat or apheresis method to obtain platelet poor and platelet concentrated fractions. The PCV, WBC count, and platelet count were compared among whole blood samples, platelet poor fractions, concentrates obtained by use of the apheresis method (ie, apheresis platelet concentrates), and concentrates obtained by use of the buffy coat method (ie, buffy coat platelet concentrates). Concentrations of transforming growth factor-beta (ie,TGF-beta1 andTGF-beta2) and insulin-like growth factor were compared between buffy coat and apheresis platelet concentrates. Results: Platelet concentrations were 8.9-fold and 5.2-fold greater in buffy coat and apheresis platelet concentrates, respectively, compared with whole blood. Platelet concentrations were 13.1-fold greater in filtered apheresis platelet concentrates, compared with whole blood. TGF-beta1 concentrations were 2.8-fold and 3.1-fold greater in buffy coat and apheresis platelet concentrates, respectively, and TGF-beta1 concentrations were 10.5-fold greater in filtered apheresis platelet concentrates, compared with whole blood. TGF-beta2 concentrations were 3.6-fold greater in apheresis platelet concentrates, compared with whole blood. Platelet concentrations correlated with growth factor concentrations across all blood and platelet fractions. White blood cell counts had a significant positive correlation with TGF-beta1 concentration in buffy coat platelet concentrates. Conclusions: Platelets and TGF-beta1 can be concentrated reliably from equine blood by use of buffy coat or apheresis methods, without modification of the protocols used for humans.
Publication Date: 2004-07-30 PubMed ID: 15281650DOI: 10.2460/ajvr.2004.65.924Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper is examining the feasibility and effectiveness of two methods for extracting platelet concentrates from equine blood: the buffy coat and apheresis methods. It discovers that both methods concentrate platelets and transforming growth factor-beta effectively from horse blood.
Methods Used in the Research
The study featured several important methods:
- The experiment involved 15 mature mixed-breed geldings as subjects.
- Blood samples were taken and processed with either the buffy coat or apheresis process to obtain different platelet fractions.
- The researchers compared the packed cell volume (PCV), white blood cell (WBC) count, and platelet count among whole blood samples, platelet-poor fractions, apheresis platelet concentrates, and buffy coat platelet concentrates.
- Additionally, the levels of insulin-like growth factor and different types of transforming growth factor-beta (TGF-beta1 and TGF-beta2) were compared between the buffy coat method and the apheresis method.
Key Results
The primary findings of the paper were:
- The platelet concentrations were significantly higher in the buffy coat (8.9-fold) and apheresis (5.2-fold) platelet concentrates compared to the whole blood samples.
- Filtered apheresis platelet concentrates yielded a remarkable 13.1-fold more platelets compared with whole blood.
- TGF-beta1 concentrations were significantly more (2.8-fold for buffy coat and 3.1-fold for apheresis) and reached up to 10.5-fold in filtered apheresis platelet concentrates.
- TGF-beta2 concentrations were 3.6-fold greater in apheresis platelet concentrates compared to whole blood.
- A strong correlation was found between platelet concentrations and growth factor concentrations across all blood and platelet fractions.
- A significant positive correlation was observed between white blood cell counts and TGF-beta1 concentration in buffy coat platelet concentrates.
Conclusions Drawn
Based on their findings, the researchers concluded:
- Both the buffy coat and apheresis methods can reliably concentrate platelets and TGF-beta1 from equine blood.
- There was no need to modify the protocols used for humans when applying these methods to equine blood.
Cite This Article
APA
Sutter WW, Kaneps AJ, Bertone AL.
(2004).
Comparison of hematologic values and transforming growth factor-beta and insulin-like growth factor concentrations in platelet concentrates obtained by use of buffy coat and apheresis methods from equine blood.
Am J Vet Res, 65(7), 924-930.
https://doi.org/10.2460/ajvr.2004.65.924 Publication
Researcher Affiliations
- Department of Clinical Sciences, Comparative Orthopedic Molecular Medicine Research Laboratories, The Ohio State University, Columbus, OH 43210, USA.
MeSH Terms
- Analysis of Variance
- Animals
- Blood Platelets / chemistry
- Blood Platelets / cytology
- Hemofiltration / instrumentation
- Hemofiltration / methods
- Horses / blood
- Leukocyte Count / veterinary
- Platelet Count / veterinary
- Plateletpheresis / methods
- Somatomedins / metabolism
- Transforming Growth Factor beta / blood
Citations
This article has been cited 15 times.- Karolczak K, Watala C. Blood Platelets as an Important but Underrated Circulating Source of TGFβ.. Int J Mol Sci 2021 Apr 26;22(9).
- Fukuda K, Kuwano A, Kuroda T, Tamura N, Mita H, Okada Y, Kasashima Y. Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma.. J Equine Sci 2020;31(4):105-111.
- Velloso Alvarez A, Boone LH, Braim AP, Taintor JS, Caldwell F, Wright JC, Wooldridge AA. A Survey of Clinical Usage of Non-steroidal Intra-Articular Therapeutics by Equine Practitioners.. Front Vet Sci 2020;7:579967.
- Fukuda K, Kuroda T, Tamura N, Mita H, Kasashima Y. Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma.. J Vet Med Sci 2020 Oct 20;82(10):1472-1479.
- Perego R, Spada E, Baggiani L, Martino PA, Proverbio D. Efficacy of a Semi Automated Commercial Closed System for Autologous Leukocyte- and Platelet-Rich Plasma (l-prp) Production in Dogs: A Preliminary Study.. Animals (Basel) 2020 Aug 4;10(8).
- Seidel SRT, Vendruscolo CP, Moreira JJ, Fülber J, Ottaiano TF, Oliva MLV, Michelacci YM, Baccarin RYA. Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?. Vet Sci 2019 Aug 21;6(3).
- Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know.. Front Vet Sci 2018;5:70.
- Gutiérrez CM, López C, Giraldo CE, Carmona JU. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma.. Vet Med Int 2017;2017:1950401.
- Hauschild G, Geburek F, Gosheger G, Eveslage M, Serrano D, Streitbürger A, Johannlükens S, Menzel D, Mischke R. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations.. BMC Vet Res 2017 Jan 5;13(1):7.
- Kisiday JD, McIlwraith CW, Rodkey WG, Frisbie DD, Steadman JR. Effects of Platelet-Rich Plasma Composition on Anabolic and Catabolic Activities in Equine Cartilage and Meniscal Explants.. Cartilage 2012 Jul;3(3):245-54.
- López C, Alvarez ME, Carmona JU. Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro Study.. Vet Med Int 2014;2014:525826.
- Perazzi A, Busetto R, Martinello T, Drigo M, Pasotto D, Cian F, Patruno M, Iacopetti I. Description of a double centrifugation tube method for concentrating canine platelets.. BMC Vet Res 2013 Jul 22;9:146.
- Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. Simple tube centrifugation for processing platelet-rich plasma in the horse.. Can Vet J 2012 Dec;53(12):1266-72.
- Giraldo CE, López C, Álvarez ME, Samudio IJ, Prades M, Carmona JU. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.. BMC Vet Res 2013 Feb 12;9:29.
- Georg R, Maria C, Gisela A, Bianca C. Autologous conditioned plasma as therapy of tendon and ligament lesions in seven horses.. J Vet Sci 2010 Jun;11(2):173-5.
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