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The Journal of general virology1994; 75 ( Pt 6); 1491-1497; doi: 10.1099/0022-1317-75-6-1491

Comparison of M and N gene sequences distinguishes variation amongst equine arteritis virus isolates.

Abstract: cDNA copies of the M and N genes of equine arteritis virus (EAV) isolates were synthesized by reverse transcription followed by polymerase chain reaction amplification. The cDNA was subjected to a cycle sequencing strategy using Taq polymerase, and the nucleotide and derived amino acid sequences of 10 virus isolates were compared. The M and N genes of all isolates had the same initiation and termination sites as the prototype Bucyrus strain and the encoded proteins were conserved between viruses. Comparison of nucleotide sequence homologies and phylogenetic tree analysis implied the existence of three EAV variants originating from the U.S.A. (Bucyrus), Austria (Vienna) and Switzerland (Bibuna), and suggested that RNA recombination between EAV isolates may have occurred.
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Publication Date: 1994-06-01 PubMed ID: 8207415DOI: 10.1099/0022-1317-75-6-1491Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on investigating the genetic differences among various isolates of Equine Arteritis Virus (EAV) by comparing their M and N genes. It suggests that there could be three EAV variants with origins in the USA, Austria, and Switzerland, and hints at the possibility of RNA recombination between EAV isolates.

Research Methodology

  • The researchers started by synthesizing cDNA copies of the M and N genes from EAV isolates. This was done through a process known as reverse transcription, followed by amplification via the polymerase chain reaction (PCR).
  • The obtained cDNA was then subjected to a cycle sequencing process using Taq polymerase, an enzyme that synthesizes DNA from its nucleotides.

Findings

  • Upon sequencing, the nucleotide and the derived amino acid sequences from 10 virus isolates were compared. This comparative analysis of the genetic sequences revealed that all isolates had the same initiation and termination sites as the prototype Bucyrus strain.
  • The proteins encoded by the M and N genes were conserved among all the analyzed EAV isolates, meaning there was a significant genetic similarity between them.

Interpretations and Implications

  • In addition to the base comparison, the researchers conducted an examination of nucleotide sequence homologies and performed a phylogenetic tree analysis. This allowed them to infer that there might exist three variants of EAV originating from different geographical regions, including the U.S.A. (with the Bucyrus strain), Austria (Vienna strain), and Switzerland (Bibuna strain).
  • The study also hinted at the possibility of RNA recombination between the various EAV isolates. This means that different EAV strains may have swapped genetic material at some point, leading to unique genetic combinations.

This research contributes important insights into the genetic makeup of EAV virus isolates, their similarities, differences, and possible mechanisms of evolution including RNA recombination. The identification of distinct virus strains also has potential implications for disease intervention strategies.

Cite This Article

APA
Chirnside ED, Wearing CM, Binns MM, Mumford JA. (1994). Comparison of M and N gene sequences distinguishes variation amongst equine arteritis virus isolates. J Gen Virol, 75 ( Pt 6), 1491-1497. https://doi.org/10.1099/0022-1317-75-6-1491

Publication

The Journal of general virology
ISSN: 0022-1317
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 75 ( Pt 6)
Pages: 1491-1497

Researcher Affiliations

Chirnside, E D
  • Department of Infectious Diseases, Animal Health Trust, Lanwades Park, Kennett, U.K.
Wearing, C M
    Binns, M M
      Mumford, J A

        MeSH Terms

        • Base Sequence
        • DNA Primers / chemistry
        • Equartevirus / genetics
        • Equartevirus / isolation & purification
        • Genes, Viral
        • Molecular Sequence Data
        • Open Reading Frames
        • Phylogeny
        • Sequence Alignment
        • Sequence Homology, Amino Acid
        • Viral Structural Proteins / genetics

        Citations

        This article has been cited 17 times.
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