Comparison of Measurements of 12 Analytes in Equine Blood Samples Using the In-Practice Falcor 350 and the Reference KoneLab 30i Analysers.
Abstract: Falcor 350 is a wet-reagent biochemistry analyser that is available for in-house use. The aim of this study was to compare the results produced by this analyser with those obtained by the KoneLab 30i that served as the reference instrument. Blood samples from 60 clinical cases were analysed for urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total calcium, phosphate, sodium, and potassium using both instruments. Good to excellent correlations (r s value) value) were identified for creatinine (0.88), total proteins (0.92), albumin (0.93), creatine kinase (0.98), aspartate aminotransferase (0.98), alkaline phosphatase (0.94), total bilirubin (0.98), phosphate (0.95), and potassium (0.97). The correlations for total calcium (0.71), sodium (0.68), and urea (0.64) were fair. For albumin, aspartate aminotransferase, creatine kinase, phosphate, potassium, total bilirubin, creatinine, and total proteins, the two instruments produce values that are closely related to each other and are sufficiently similar to allow them to be used interchangeably without the need for additional correction factor computations. Because of differences in the methodologies, the Falcor results for alkaline phosphatase, total calcium, and sodium cannot be used interchangeably and should be interpreted using reference intervals established from the Falcor analyser.
Publication Date: 2012-09-27 PubMed ID: 23762583PubMed Central: PMC3671722DOI: 10.5402/2012/475419Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research compared the performance of two laboratory instruments, Falcor 350 and KoneLab 30i, in measuring various substances in horse blood samples and found that while there was good correlation in measuring some substances, for others, the readings differed and could not be used interchangeably.
Research Method and Objective
- The purpose of this study was to evaluate the performance of the Falcor 350, a wet-reagent biochemistry analyser, by comparing its results to those of the KoneLab 30i, a more established and trusted laboratory instrument.
- The researchers are interested in finding out how well the Falcor 350 could measure 12 specific analytes in equine (horse) blood samples, a crucial factor for vets who might consider using this new analyser for praxis.
- The analytes in question were urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total calcium, phosphate, sodium, and potassium – all essential indicators of horse health.
Comparative Analysis of Instruments
- The researchers conducted their comparison using blood samples from 60 clinical cases and analysed each sample using both instruments.
- The accuracy of each instrument’s measurements was compared using correlation, with a high correlation indicating similar results between the two machines.
- Most of the analytes (creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, phosphate, and potassium) showed high correlations, suggesting that the Falcor 350 can accurately measure these substances in horse blood.
Differences and Recommendations
- However, the measurements for total calcium, sodium, and urea were only fairly correlated, indicating that these results were not consistent between the two machines.
- For these three substances, readings from the Falcor 350 should not be used interchangeably with those from the KoneLab 30i. Vets should interpret the Falcor results using separate reference intervals established for this particular analyser.
- For the remaining nine substances, the two instruments produced closely related measurements, meaning that they can be used interchangeably without the need for additional computations or corrections.
Cite This Article
APA
Papasouliotis K, Tennant KV, Dodkin S, Mason J.
(2012).
Comparison of Measurements of 12 Analytes in Equine Blood Samples Using the In-Practice Falcor 350 and the Reference KoneLab 30i Analysers.
ISRN Vet Sci, 2012, 475419.
https://doi.org/10.5402/2012/475419 Publication
Researcher Affiliations
- Diagnostic Laboratories, Langford Veterinary Services, University of Bristol, Langford, Bristol BS40 5DU, UK ; School of Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK.
References
This article includes 17 references
- Belford CJ, Lumsden JH. Assessment of a reflectance photometer in a veterinary laboratory.. Can Vet J 1984 Jun;25(6):243-6.
- Tschudi P. [The Seralyzer dry-reagent system and its applicability to veterinary practice].. Tierarztl Prax 1985;13(3):385-93.
- Hoshi F, Satho M, Koyama S, Nakadaka K, Chiba M, Ikeda N, Hakamada R, Higuchi S, Kawamura S. Application to cows and horses of Spotchem, a dry-chemistry blood analyzer for use in veterinary clinics.. Zentralbl Veterinarmed A 1994 Feb;41(1):22-30.
- Tschudi PR. [Evaluation of the dry chemical analysis system VetTest 8008].. Schweiz Arch Tierheilkd 1995;137(8):381-5.
- Lopes-Pereira CM, Harun M, Schmidtova D, Timane I, Mabasso M, Otto F, Bogin E. Use of the dry chemistry "Reflotron" blood analyzer under outdoor-field conditions in veterinary medicine.. Eur J Clin Chem Clin Biochem 1996 Mar;34(3):231-5.
- Papasouliotis K, Dodkin S, Murphy K, Woloszczuk G, Fleming M. Analysis of canine and feline blood samples using the Kuadro in-house wet-reagent chemistry analyser.. J Small Anim Pract 2006 Apr;47(4):190-5.
- Bienzle D, Stanton JB, Embry JM, Bush SE, Mahaffey EA. Evaluation of an in-house centrifugal hematology analyzer for use in veterinary practice.. J Am Vet Med Assoc 2000 Oct 15;217(8):1195-200.
- Dewhurst EC, Crawford E, Cue S, Dodkin S, German AJ, Papasouliotis K. Analysis of canine and feline haemograms using the VetScan HMT analyser.. J Small Anim Pract 2003 Oct;44(10):443-8.
- Sutton A, Dawson H, Hoff B, Grift E, Shoukri M. Analyte comparisons between 2 clinical chemistry analyzers.. Can Vet J 1999 Apr;40(4):255-60.
- Tvedten H, Weiss D. The complete blood count and bone marrow examination: general comments and selected techniques. In: Willard MD, Tvedten H, Turnwald GH, Saunders WB, editors. Small Animal Clinical Diagnosis by Laboratory Methods. 3rd edition. Philadelphia, Pa, USA: 1999. pp. 11–30.
- Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement.. Lancet 1986 Feb 8;1(8476):307-10.
- Jensen AL, Bantz M. Comparing laboratory tests using the difference plot method.. Vet Clin Pathol 1993;22(2):46-48.
- Gray TE, Pratt MC, Cusick PK. Determination of Agreement Between Laboratory Instruments.. Contemp Top Lab Anim Sci 1999 Mar;38(2):56-59.
- Papasouliotis K, Dodkin S, Murphy KF, Sladen A. Comparison of measurements of 18 analytes in canine and feline blood samples using the in-practice Falcor 350 and the reference KoneLab 30i analysers.. J Small Anim Pract 2008 Oct;49(10):494-501.
- James TJ. Ion-selective electrodes.. Postgrad Med J 1999 Apr;75(882):254.
- Loughrey CM, Hanna EV, McDonnell M, Archbold GP. Sodium measurement: effects of differing sampling and analytical methods.. Ann Clin Biochem 2006 Nov;43(Pt 6):488-93.
- Moss DW, Henderson AR. Enzymes. In: Burtis CA, Ashwood ER, Saunders WB, editors. Tietz Textbook of Clinical Chemistry. 2nd edition. Philadelphia, Pa, USA: 1994. pp. 735–896.
Citations
This article has been cited 1 times.- Brans M, Marynissen S, Mortier F, Duchateau L, Daminet S, Paepe D. Effect of storage temperature and time on measurement of serum symmetric dimethylarginine concentration using point-of-care and commercial laboratory analyzers in cats and dogs. J Vet Intern Med 2023 Sep-Oct;37(5):1794-1805.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
