Comparison of pH, lactate, and glucose analysis of equine synovial fluid using a portable clinical analyzer with a bench-top blood gas analyzer.
Abstract: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Methods: Prospective experimental study. Methods: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.
© Copyright 2011 by The American College of Veterinary Surgeons.
Publication Date: 2011-07-19 PubMed ID: 21770979DOI: 10.1111/j.1532-950X.2011.00854.xGoogle Scholar: Lookup
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- Journal Article
- Analytical Methods
- Clinical Examination
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Equine Health
- Glucose
- Horses
- Laboratory Methods
- Lactate
- pH
- Sepsis
- Synovial Fluid
- Veterinary Medicine
- Veterinary Practice
- Veterinary Procedure
- Veterinary Research
Summary
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This research paper compares the efficiency of a portable clinical analyzer with a laboratory-based bench-top analyzer in analyzing pH, lactate, and glucose concentrations in synovial fluid from horses. The study concludes that the portable analyzer performs comparably to the lab-based tool for lactate and glucose measurements, but less so for pH.
Objective of the study
- The primary goal of this research was to compare the performance and accuracy of a portable clinical analyzer and a laboratory-based bench-top analyzer in determining the levels of pH, lactate, and glucose in equine synovial fluid.
Methods used in the study
- The study involved several horses under different categories: clinically normal horses, horses euthanized for reasons unrelated to the study, horses that had synoviocentesis for purposes other than sepsis identification, horses that had synoviocentesis for sepsis evaluation, and a few horses without any recorded clinical data.
- From each synovial fluid sample, the supernatant was collected and analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and an i-STAT portable clinical analyzer.
- To determine the bias and agreement between the two analyzers, the researchers constructed Bland-Altman plots and performed concordance analysis.
Results and Conclusions
- The study found acceptable agreement between the two analyzers for lactate and glucose concentrations; their biases were 0.198 mmol/L and 9 mg/dL, with concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively.
- However, the agreement between the two machines for pH analysis was not satisfactory. The bias here was -0.057 with a concordance correlation coefficient of 0.89.
- Overall, the research concluded that the portable clinical analyzer performs similarly to the laboratory-based bench-top analyzer for assessing lactate and glucose concentrations in synovial fluid of horses, but not for the pH level.
Cite This Article
APA
Dechant JE, Symm WA, Nieto JE.
(2011).
Comparison of pH, lactate, and glucose analysis of equine synovial fluid using a portable clinical analyzer with a bench-top blood gas analyzer.
Vet Surg, 40(7), 811-816.
https://doi.org/10.1111/j.1532-950X.2011.00854.x Publication
Researcher Affiliations
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, CA 95616, USA. jedechant@ucdavis.edu
MeSH Terms
- Animals
- Bacterial Infections / diagnosis
- Bacterial Infections / metabolism
- Bacterial Infections / veterinary
- Blood Gas Analysis / instrumentation
- Blood Gas Analysis / veterinary
- Glucose / analysis
- Horse Diseases / metabolism
- Horses
- Hydrogen-Ion Concentration
- Joint Diseases / diagnosis
- Joint Diseases / veterinary
- Lactic Acid / analysis
- Synovial Fluid / chemistry
Citations
This article has been cited 6 times.- Stabile M, Girelli CR, Lacitignola L, Samarelli R, Crovace A, Fanizzi FP, Staffieri F. (1)H-NMR metabolomic profile of healthy and osteoarthritic canine synovial fluid before and after UC-II supplementation. Sci Rep 2022 Nov 16;12(1):19716.
- Avellar HK, Lutter JD, Ganta CK, Beard W, Smith JR, Jonnalagadda N, Peloquin S, Kang Q, Ayub K. In vitro antimicrobial activity of equine platelet lysate and mesenchymal stromal cells against common clinical pathogens. Can J Vet Res 2022 Jan;86(1):59-64.
- Douzi W, Guillot X, Bon D, Seguin F, Boildieu N, Wendling D, Tordi N, Dupuy O, Dugué B. (1)H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients. Metabolites 2020 Nov 13;10(11).
- Hidalgo AI, Carretta MD, Alarcón P, Manosalva C, Müller A, Navarro M, Hidalgo MA, Kaehne T, Taubert A, Hermosilla CR, Burgos RA. Pro-inflammatory mediators and neutrophils are increased in synovial fluid from heifers with acute ruminal acidosis. BMC Vet Res 2019 Jul 3;15(1):225.
- Tsukano K, Sarashina S, Suzuki K. Hypoglycemia and failure of respiratory compensation are risk factors for mortality in diarrheic calves in Hokkaido, northern Japan. J Vet Med Sci 2018 Jul 18;80(7):1159-1164.
- Londeree W, Davis K, Helman D, Abadie J. Bodily fluid analysis of non-serum samples using point-of-care testing with iSTAT and Piccolo analyzers versus a fixed hospital chemistry analytical platform. Hawaii J Med Public Health 2014 Sep;73(9 Suppl 1):3-8.
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