[Comparison of proviral genomes between the Chinese EIAV donkey leukocyte-attenuated vaccine and its parental virulent strain].
Abstract: The donkey leukocyte-attenuated vaccine of equine infectious anemia virus (EIAV) was the first lentiviral vaccine that induced solid protection from the infection of virulent strains. To elucidate the mechanism of increased immunogenicity and attenuated virulence of the vaccine, the proviral genomic DNA of an EIAV vaccine strain, EIAV(DLV121) was analyzed and compared with the genome of a parental virulent strain EIAV(DV117). Full length viral genomic DNAs were amplified as two segments by LA-PCR and were cloned. Because of the genomic diversity of retroviral quasispecies, 10 full-length sequences of EIAV(DLV121) and 4 full-length sequences of EIAV(DV117) from randomly picked clones were analyzed. Results showed that the average length of the complete nucleotide sequence of EIAV(DLV121) was 8,236bp and EIAV(DV117) was 8,249bp, with the inter-strain diversity of 2.8%. As for individual genes between the vaccine and virulent strains, the differences in nucleotide sequence of S2, LTR and env were significantly higher than the other genes with the diversity of 4.1%, 3.7% and 3.1%, respectively. Considerable variations in deduced amino acid sequences were found in S2, S3 and env. The diversities were 10.4%, 5.6% and 4.8%, respectively. Furthermore, the LTR of EIAV(DLV121) consisted of at least 5 subtypes grouped by their nucleotide sequences. There were two additional N-linked glycosylation sites in the deduced amino acid sequence of EIAV(DV117) gp90 than that of EIAV(DLV121). Among glycosylation sites in the gp90 of virulent strain, 3 were found unique only in EIAV(DV117), of which 2 were located in the principle neutralizing domain (PND). In addition, there was one EIAV(DLV121) -specific glycosylation site, which was positioned in the PND, too. Taken together, it is clear that greatly increased genomic diversity exists in the EIAV vaccine strain, which provides important information for the further study on biological characters of the Chinese EIAV attenuated vaccine.
Publication Date: 2009-02-21 PubMed ID: 19226953 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- English Abstract
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study involves a comparative analysis of the genomes between the Chinese EIAV (equine infectious anemia virus) donkey leukocyte-attenuated vaccine and its parental virulent strain. The aim of this study is to understand the increased immunogenicity and attenuated virulence of the vaccine.
Methodology
- The genomic DNA of an EIAV vaccine strain, EIAV(DLV121), was examined and compared with the genome of a parental virulent strain EIAV(DV117).
- Full-length viral genomic DNAs were amplified as two segments via LA-PCR (Long Amplification-Polymerase Chain Reaction) and were cloned.
- To address the genetic diversity of retroviral quasispecies, 10 full-length sequences of EIAV(DLV121) and 4 full-length sequences of EIAV(DV117) from randomly chosen clones were studied.
Results
- Results indicated that the average length of the full nucleotide sequence of EIAV(DLV121) was 8,236bp and for EIAV(DV117) it was 8,249bp, with an inter-strain diversity of 2.8%.
- For individual genes between the vaccine and virulent strains, significant differences in the nucleotide sequence of S2, LTR and env were found, with diversities of 4.1%, 3.7%, and 3.1% respectively.
- Important variations were discovered in deduced amino acid sequences in S2, S3, and env, with diversities of 10.4%, 5.6%, and 4.8% respectively.
- The LTR of EIAV(DLV121) consisted of at least 5 subtypes grouped by their nucleotide sequences.
- The EIAV(DV117) gp90 deduced amino acid sequence had two extra N-linked glycosylation sites compared to EIAV(DLV121).
- Among glycosylation sites in the gp90 of the virulent strain, 3 were unique to EIAV(DV117), of which 2 were located in the principle neutralizing domain (PND).
- The EIAV(DLV121) had one specific glycosylation site, also located in the PND.
Conclusion
- The EIAV vaccine strain presents an increased genomic diversity. This information is crucial for the further study on the biological characteristics of the Chinese EIAV attenuated vaccine.
Cite This Article
APA
Wang XF, Jiang CG, Guo W, Xiang W, Lv XL, Zhao LP, Wang FL, Kong XG, Zhang XY, Shao YM, Zhou JH.
(2009).
[Comparison of proviral genomes between the Chinese EIAV donkey leukocyte-attenuated vaccine and its parental virulent strain].
Bing Du Xue Bao, 24(6), 443-450.
Publication
Researcher Affiliations
- Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Equidae
- Genome, Viral
- Infectious Anemia Virus, Equine / chemistry
- Infectious Anemia Virus, Equine / genetics
- Infectious Anemia Virus, Equine / immunology
- Leukocytes / immunology
- Leukocytes / virology
- Molecular Sequence Data
- Sequence Alignment
- Vaccines, Attenuated / chemistry
- Vaccines, Attenuated / genetics
- Vaccines, Attenuated / immunology
- Viral Proteins / chemistry
- Viral Proteins / genetics
- Viral Proteins / immunology
- Virulence
Citations
This article has been cited 4 times.- Wang HN, Rao D, Fu XQ, Hu MM, Dong JG. Equine infectious anemia virus in China. Oncotarget 2018 Jan 2;9(1):1356-1364.
- Han X, Zhang P, Yu W, Xiang W, Li X. Amino acid mutations in the env gp90 protein that modify N-linked glycosylation of the Chinese EIAV vaccine strain enhance resistance to neutralizing antibodies. Virus Genes 2016 Dec;52(6):814-822.
- Liu Q, Ma J, Wang XF, Xiao F, Li LJ, Zhang JE, Lin YZ, Du C, He XJ, Wang X, Zhou JH. Infection with equine infectious anemia virus vaccine strain EIAVDLV121 causes no visible histopathological lesions in target organs in association with restricted viral replication and unique cytokine response. Vet Immunol Immunopathol 2016 Feb;170:30-40.
- Wang X, Wang S, Lin Y, Jiang C, Ma J, Zhao L, Lv X, Wang F, Shen R, Zhou J. Unique evolution characteristics of the envelope protein of EIAV(LN₄₀), a virulent strain of equine infectious anemia virus. Virus Genes 2011 Apr;42(2):220-8.
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