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The Journal of veterinary medical science2021; 84(1); 129-132; doi: 10.1292/jvms.21-0539

Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.

Abstract: Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
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Publication Date: 2021-12-02 PubMed ID: 34853198PubMed Central: PMC8810321DOI: 10.1292/jvms.21-0539Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the effectiveness of seven different tests for detecting Taylorella equigenitalis, an agent causing disease in horses. The study found that real-time PCR and semi-nested PCR were the most reliable methods for detecting the pathogen both in terms of sensitivity and efficiency.

Research Background and Aim

  • The study primarily centers on Taylorella equigenitalis, a bacterium that causes contagious equine metritis, a severe disease in horses.
  • The objective of the research was to identify the most reliable and quick diagnostic method for detecting T. equigenitalis. To achieve this, the researchers compared seven different nucleic acid amplification tests.

Methods and Results

  • Among the seven tests examined, they included real-time PCR, semi-nested PCR, and several conventional PCRs (cPCRs).
  • Their experiment involved evaluating the 95% detection limits of each of the seven assays and found that these varied significantly among the different tests.
  • Specifically, real-time PCR showed the lowest detection limit at 0.77 fg/reaction, making it more sensitive than the other tests. Some of the conventional PCR detection limits were above 100 fg/reaction, indicating their lower sensitivity.
  • The study also used experimentally infected samples to compare the performances of these tests. Both real-time PCR and semi-nested PCR returned the highest positive results, detecting T. equigenitalis in 33 out of 42 samples.
  • However, two of the conventional PCRs showed significantly poor results, detecting only 2 and 7 positive results respectively from the same set of infected samples.

Conclusion

  • Based on their findings, the researchers concluded that sensitive molecular assays such as real-time PCR and semi-nested PCR are crucial for the efficient detection of T. equigenitalis in clinical samples.
  • The study highlights the variability in test performance and underlines the need for continued evaluation and refinement of diagnostic techniques in the field of veterinary medicine.

Cite This Article

APA
Kinoshita Y, Kakoi H, Ishige T, Yamanaka T, Niwa H, Uchida-Fujii E, Nukada T, Ueno T. (2021). Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis. J Vet Med Sci, 84(1), 129-132. https://doi.org/10.1292/jvms.21-0539

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 84
Issue: 1
Pages: 129-132

Researcher Affiliations

Kinoshita, Yuta
  • Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Kakoi, Hironaga
  • Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
Ishige, Taichiro
  • Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
Yamanaka, Takashi
  • Epizootic Prevention Section, Equine Department, Japan Racing Association, 1-1-1 Nishi Shinnbashi, Minato-ku, Tokyo 105-0003, Japan.
Niwa, Hidekazu
  • Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Uchida-Fujii, Eri
  • Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Nukada, Toshio
  • Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Ueno, Takanori
  • Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.

MeSH Terms

  • Animals
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / veterinary
  • Horse Diseases / diagnosis
  • Horses
  • Nucleic Acid Amplification Techniques / veterinary
  • Real-Time Polymerase Chain Reaction / veterinary
  • Taylorella equigenitalis / genetics

Conflict of Interest Statement

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors that may pose a conflict of interest.

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Citations

This article has been cited 1 times.
  1. Knox A, Zerna G, Beddoe T. Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP). Animals (Basel) 2023 Aug 18;13(16).
    doi: 10.3390/ani13162663pubmed: 37627456google scholar: lookup
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