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Virus research1996; 43(1); 17-31; doi: 10.1016/0168-1702(96)01308-1

Comparison of the deduced matrix and fusion protein sequences of equine morbillivirus with cognate genes of the Paramyxoviridae.

Abstract: The nucleotide sequence of the matrix protein of equine morbillivirus (EMV) was determined to be 1062 nucleotides and coded for a deduced protein of M(r) 40148 having a net charge of + 19 at neutral pH. The matrix protein gene was separated from the P and F genes by intercistronic regions of 546 and 469 nucleotides, respectively. The nucleotide sequence which coded for the F protein was 1641 nucleotides and coded for a deduced protein of 546 amino acids having an M(r) of 60,447 and a charge + 4 at neutral pH. Partial sequence information was also determined for the P/V proteins. M, P and F protein sequence comparisons revealed that a greater homology existed between EMV and known members of the morbillivirus genus than with other members of the Paramyxoviridae and that this homology resided within the central half of the protein for the fusion protein, the C-terminal half of the matrix protein and at certain sites with the P protein. Far greater homology was seen between the morbilliviruses and EMV than for the other paramyxoviridae. It was inferred from phylogenetic analyses that EMV was a distantly related member of the morbillivirus genus. A conserved sequence of 18 nucleotides (assumed to be the transcriptional editing site) was present in the P gene of EMV. Insertion of a single nucleotide residue within this site generated the C-terminus of a V-like, cysteine rich protein. Likewise, a conserved 'CTT' intergenic region presumed to be the transcription termination and polyadenylation signal was present in EMV between the P-M-F genes. The close sequence homology of these sites with that of morbilliviruses also inferred that EMV was a member of the morbillivirus genus.
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Publication Date: 1996-07-01 PubMed ID: 8822631DOI: 10.1016/0168-1702(96)01308-1Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper deals primarily with the genetic analysis of the equine morbillivirus (EMV), comparing the nucleotide sequences of its matrix protein and fusion protein with the cognate genes found in the Paramyxoviridae family.

Decoding the Equine Morbillivirus

  • The research begins by decoding the genetic structure of the EMV’s matrix protein. This gene was found to be 1062 nucleotides long and to encode a deduced protein which had a molecular weight (M(r)) of 40148 and a net charge of +19 at neutral pH.
  • The matrix protein gene was separated from the P and F genes by intercistronic regions of 546 and 469 nucleotides respectively. This suggests distinct roles for these proteins in the replication and transcription of the EMV.
  • In addition, the nucleotide sequence for the F protein was identified to be 1641 nucleotides long and to code for a deduced protein of 546 amino acids, with an M(r) of 60,447 and a charge of +4 at neutral pH. The partial sequence information for the P/V proteins was also determined.

Comparing with the Paramyxoviridae

  • When the sequences of the M, P, and F proteins were compared, greater homology was found between the EMV and known members of the morbillivirus genus than with other members of the Paramyxoviridae.
  • The homology was stronger in the central part of the fusion protein, the C-terminal part of the matrix protein, and at certain sites within the P protein.
  • Through phylogenetic analyses, the researchers inferred that the EMV can be considered a distant relative of the morbillivirus genus.

Identifying Shared Genetic Markers

  • The researchers identified an 18 nucleotide-long conserved sequence, assumed to be the transcriptional editing site, within the P gene of the EMV.
  • A single nucleotide insertion within this site resulted in the creation of a C-terminus of a V-like, cysteine-rich protein.
  • Finally, a conserved ‘CTT’ intergenic region, believed to be the transcription termination and polyadenylation signal, was identified between the P-M-F genes.
  • The presence of these markers, along with the overall homology of sequences, strongly suggests that EMV is indeed a member of the morbillivirus genus.

Cite This Article

APA
Gould AR. (1996). Comparison of the deduced matrix and fusion protein sequences of equine morbillivirus with cognate genes of the Paramyxoviridae. Virus Res, 43(1), 17-31. https://doi.org/10.1016/0168-1702(96)01308-1

Publication

Virus research
ISSN: 0168-1702
NlmUniqueID: 8410979
Country: Netherlands
Language: English
Volume: 43
Issue: 1
Pages: 17-31

Researcher Affiliations

Gould, A R
  • CSIRO, Australian Animal Health Laboratory, Geelong, Australia.

MeSH Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Viral
  • Horses / virology
  • Molecular Sequence Data
  • Morbillivirus / classification
  • Morbillivirus / genetics
  • Paramyxoviridae / classification
  • Paramyxoviridae / genetics
  • Phylogeny
  • Viral Fusion Proteins / genetics
  • Viral Matrix Proteins / genetics

Citations

This article has been cited 19 times.
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