Comparison of the lactate minimum speed and the maximal lactate steady state to determine aerobic capacity in purebred Arabian horses.
Abstract: To compare five different protocols for estimating the lactate minimum speed (LMS) with that for estimating the maximal lactate steady state (MLSS) in Arabian horses, in order to obtain a more rapid method for monitoring aerobic capacity and prescribing training schedules. Methods: Eight purebred Arabian horses were conditioned to exercise on a treadmill for 12 days then submitted to three to five exercise sessions to determine the MLSS. Blood samples were collected from a jugular catheter at specific intervals for measurement of lactate concentrations. The MLSS was the velocity maintained during the last 20 minutes of constant submaximal exercise, at which the concentration of lactate increased by no more than 1.0 mmol/L. The LMS test protocols (P1 - P5) included a warm-up period followed by a high-intensity gallop. The speed was then reduced to 4 m/s, and the incremental portion of the test was initiated. In P1, P2, and P3, the velocity increment was 0.5 m/s, and the duration of each incremental stage was three, five and seven minutes, respectively. In P4 and P5, the velocity increments were 1.0 and 1.5 m/s, respectively, and the duration of the stages was fixed at five minutes each. A second-degree polynomial function was fitted to the lactate-velocity curve, and the velocity corresponding to the lowest concentration of lactate was the LMS. Results: Only the mean LMS determined by P1 and P2 did not differ from the velocity determined by the MLSS test (p > 0.1). There was a strong correlation (r >0.6) between P1 and the MLSS velocity. A limits of agreement plot revealed that the best agreement occurred between the MLSS test and P1 (mean bias = 0.14 m/s), followed by P2 (bias = -0.22 m/s). The lactate concentrations associated with the various LMS protocols did not differ. Conclusions: This study shows the variation between protocols of the LMS test for determining the onset of blood lactate accumulation but also reveals that, at least for Arabian horses, the P1 protocol of the LMS has good agreement with the MLSS.
Publication Date: 2013-07-22 PubMed ID: 23869425DOI: 10.1080/00480169.2013.815103Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research aims to compare different protocols to find out a quick and efficient way of estimating the aerobic capacity of Arabian horses by determining the lactate minimum speed (LMS) and comparing it to the maximal lactate steady state (MLSS).
Methods
- Eight purebred Arabian horses were made to exercise on a treadmill for 12 days, with three to five exercise sessions designated to determine the MLSS.
- Blood samples were collected at different intervals from a jugular catheter for the measurement of lactate concentrations.
- The MLSS was the velocity that the horses maintained during the last 20 minutes of constant submaximal exercise, during which the lactate concentration did not increase by more than 1.0 mmol/L.
- For the LMS test, five different protocols were designed, termed as P1 – P5. Each protocol started with a warm-up period, which was then followed by a high-intensity sprint. The pace was then slowed down to 4 m/s, and the incremental portion of the test was initiated.
- Under P1, P2, and P3, the velocity increment was set at 0.5 m/s, and the time duration for each incremental stage was different: three minutes for P1, five minutes for P2, and seven minutes for P3.
- In contrast to the previous protocols, for P4 and P5, the velocity increments were set at 1.0 and 1.5 m/s, respectively. However, the duration of the stages in these protocols was fixed at five minutes each.
- The LMS was then calculated on the basis of lactate-velocity curve by fitting a second-degree polynomial function.
Results
- Results indicated that only the average LMS determined by the P1 and P2 protocols did not significantly differ from the MLSS velocity (p > 0.1).
- The correlation between P1 and the MLSS velocity was found to be strong (r >0.6).
- A limits of agreement plot showed that the best agreement occurred between the MLSS test and P1 (with a mean bias of 0.14 m/s), followed by P2 (with a bias of -0.22 m/s).
- The lactate concentrations associated with different LMS protocols were not found to differ from each other.
Conclusions
- The study draws a conclusion that there is a degree of variation between different LMS test protocols in determining the onset of blood lactate accumulation.
- However, at least for Arabian horses, the P1 protocol of the LMS test has a good agreement with the MLSS, indicating it to be a successful method of determining aerobic capacity in these horses rapidly and efficiently.
Cite This Article
APA
Miranda MC, Queiroz-Neto A, Silva-Júnior JR, Pereira MC, Soares OA, Borghi RT, Ferraz GC.
(2013).
Comparison of the lactate minimum speed and the maximal lactate steady state to determine aerobic capacity in purebred Arabian horses.
N Z Vet J, 62(1), 15-20.
https://doi.org/10.1080/00480169.2013.815103 Publication
Researcher Affiliations
- a Departamento das Clínicas Veterinárias, Centro de Ciências Agrárias , Universidade Estadual do Maranhão, Cidade Universitária Paulo VI , Tirirical, CEP 65055-970 , São Luís , MA , Brazil.
MeSH Terms
- Animals
- Female
- Horses / blood
- Horses / genetics
- Horses / physiology
- Lactic Acid / blood
- Male
- Muscle, Skeletal / physiology
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 4 times.- Spinella G, Valentini S, Matarazzo M, Tidu L, Ferlizza E, Isani G, Andreani G. Effects of exercise on urinary biochemical parameters and proteins in a group of well-trained military working dogs.. Vet Q 2023 Dec;43(1):1-9.
- Siegers E, van Wijk E, van den Broek J, Sloet van Oldruitenborgh-Oosterbaan M, Munsters C. Longitudinal Training and Workload Assessment in Young Friesian Stallions in Relation to Fitness: Part 1.. Animals (Basel) 2023 Feb 16;13(4).
- De Maré L, Boshuizen B, Vidal Moreno de Vega C, de Meeûs C, Plancke L, Gansemans Y, Van Nieuwerburgh F, Deforce D, de Oliveira JE, Hosotani G, Oosterlinck M, Delesalle C. Profiling the Aerobic Window of Horses in Response to Training by Means of a Modified Lactate Minimum Speed Test: Flatten the Curve.. Front Physiol 2022;13:792052.
- Messias LHD, Gobatto CA, Beck WR, Manchado-Gobatto FB. The Lactate Minimum Test: Concept, Methodological Aspects and Insights for Future Investigations in Human and Animal Models.. Front Physiol 2017;8:389.
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