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Home / Equine Research Bank / Can J Vet Res / Comparison of the oral and rectal mucosal and colonic serosa...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2018; 82(1); 55-59;

Comparison of the oral and rectal mucosal and colonic serosal microcirculations of healthy, anesthetized horses.

Abstract: The objectives of the study were to: i) determine baseline microvascular perfusion indices (MPI) and assess their repeatability in healthy horses under general anesthesia, and ii) compare the MPIs of 3 microvascular beds (oral mucosa, colonic serosa, and rectal mucosa). Healthy adult horses were anesthetized and sidestream dark field microscopy was used to collect video loops of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions without cardiovascular support drugs; videos were later analyzed to produce MPIs. Baseline MPI values were determined for each site, which included the total vessel density (TVD), perfused vessel density (PVD), portion perfused vessels (PPV), and microcirculatory flow index (MFI). Differences in MPIs between microvascular beds were not statistically significant. Repeatability of the measurements varied for each MPI. In particular, the site of sampling had a profound effect on the repeatability of the PPV measurements and should be considered in future studies. Les objectifs de cet étude étaient: i) de déterminer les indices de perfusion microvasculaires (IPM) de base et évaluer leur répétabilité chez les chevaux en bonne santé sous anesthésie générale, et ii) de comparer les IPMs de trois lits microvasculaires (muqueuse orale, séreuse du colon, et muqueuse rectale). Des chevaux adultes en bonne santé ont été anesthésiés et une unité de microscopie au champ sombre a été utilisée pour recueillir des boucles vidéo de la muqueuse buccale, de la muqueuse rectale, et de la séreuse du colon sous des conditions de tension artérielle normale. Les vidéos ont été analysées pour produire les IPMs, incluant la densité totale des vaisseaux, la densité des vaisseaux perfusés, la portion des vaisseaux perfusés, et l’index de flux microcirculaire. Pour chaque IPM, les différences entre les sites anatomiques n’étaient pas significatives statistiquement. La répétabilité des mesures variait pour chaque IPM. En particulier, le type de lit microvasculaire a une influence profonde sur la répétabilité des mesures.(Traduit par les auteurs).
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Publication Date: 2018-02-01 PubMed ID: 29382969PubMed Central: PMC5764044
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on comparing the microvascular perfusion and its repeatability in three different areas of the body (oral mucosa, colonic serosa and rectal mucosa) in healthy, anesthetized horses. The results found no significant difference in microvascular perfusion between the three areas and informed that repeatability varied between measurements.

Study Objectives

  • The primary aim of this research was to establish baseline microvascular perfusion indices (MPI) for healthy anesthetized horses and to assess the repeatability of these measurements.
  • The researchers also sought to compare MPIs from three different microvascular beds including the oral mucosa, the colonic serosa, and the rectal mucosa in anesthetized horses.

Methodology

  • Adult horses in good health were anesthetized as part of the process, and their blood flow was maintained under normotensive conditions without the use of cardiovascular support drugs.
  • The research utilized sidestream dark field microscopy, a specialized method for visualizing the microcirculation, to collect video loops from the three specified locations in these anesthetized horses.
  • These video loops then were analysed to produce MPIs.

Microvascular Perfusion Indices

  • The team considered four main MPI variables. These were total vessel density (TVD), perfused vessel density (PVD), and the fraction of perfused vessels (PPV).
  • Another index was the microcirculatory flow index (MFI), a measurement that provides an assessment of the blood flow at the microvascular level.
  • For each test site, these MPIs were calculated, providing a snapshot of the microcirculation within these tissues.

Study Findings

  • Statistical analysis revealed no significant differences in the different MPIs between the different microvascular beds being measured.
  • However, the repeatability of the measurements varied depending on the MPI. This suggests that while the baseline measurements are statistically similar, how closely subsequent measurements might match can vary.
  • The location of the sample had a significant impact on the repeatability of the PPV measurements, which must be taken into account in future studies to ensure the reliability of data.

Cite This Article

APA
Kieffer PJ, Williams JM, Shepard MK, Giguère S, Epstein KL. (2018). Comparison of the oral and rectal mucosal and colonic serosal microcirculations of healthy, anesthetized horses. Can J Vet Res, 82(1), 55-59.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 1928-9022
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 82
Issue: 1
Pages: 55-59

Researcher Affiliations

Kieffer, Philip J
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).
Williams, Jarred M
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).
Shepard, Molly K
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).
Giguère, Steeve
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).
Epstein, Kira L
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA (Kieffer, Williams, Giguère, Epstein); MedVet Medical & Cancer Centers for Pets, Chicago, Illinois, USA (Shepard).

MeSH Terms

  • Anesthesia, General / veterinary
  • Animals
  • Blood Pressure
  • Colon / blood supply
  • Female
  • Horses / physiology
  • Intestinal Mucosa / blood supply
  • Male
  • Microcirculation
  • Mouth Mucosa / blood supply
  • Rectum / blood supply
  • Video Recording

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Citations

This article has been cited 5 times.
  1. Freccero F, Di Maio C, Mariella J, Lanci A, Castagnetti C, Hallowell G. Assessment of the microvascular perfusion using sidestream dark-field imaging in healthy newborn foals. Vet Med Sci 2023 Jan;9(1):158-166.
    doi: 10.1002/vms3.1051pubmed: 36524796google scholar: lookup
  2. Yozova ID, Londoño LA, Millar KK, Sano H, Weidgraaf K, Thomson NA, Munday JS. Rapid Patient-Side Evaluation of Endothelial Glycocalyx Thickness in Healthy Sedated Cats Using GlycoCheck® Software. Front Vet Sci 2021;8:727063.
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  3. Chow RS. Terms, Definitions, Nomenclature, and Routes of Fluid Administration. Front Vet Sci 2020;7:591218.
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  4. Sauter PK, Steblaj B, Kästner SBR, Söbbeler FJ, Reiners JK, Kutter APN, Bautitsta AJG, Neudeck S. Changes in microcirculation variables in an acute endotoxaemic equine model. Equine Vet J 2025 Nov;57(6):1623-1634.
    doi: 10.1111/evj.14473pubmed: 39844573google scholar: lookup
  5. Kieffer PJ, Williams JM, Shepard MK, Giguère S, Epstein KL. Effect of Hypotension and Dobutamine on Gastrointestinal Microcirculations of Healthy, Anesthetized Horses. Vet Sci 2024 Feb 19;11(2).
    doi: 10.3390/vetsci11020095pubmed: 38393113google scholar: lookup
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