Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.
Abstract: In the past decade, mass spectrometry has become an important technology for protein identification. Recent developments in mass spectrometry allow a large number of identifications in samples; therefore, mass-spectrometry-based techniques have been applied to the discovery of biomarkers. Here, we conducted a proteomic study to compare the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport (RDT). We found that four proteins, apolipoprotein F, lipopolysaccharide binding protein, lysozyme and protein S100-A8, were upregulated, while keratin 1 was downregulated in the RDT group. It is assumed that inflammation and immune response are involved in the changes of these proteins. The findings suggested that these proteins are potentially useful for elucidating the mechanism of development of RDT.
©2021 The Japanese Society of Equine Science.
Publication Date: 2021-03-16 PubMed ID: 33776535PubMed Central: PMC7984915DOI: 10.1294/jes.32.11Google Scholar: Lookup
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Summary
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This research article explores the differences in protein composition between the blood of healthy horses and horses with a travel-related respiratory disease, using mass spectrometry for protein identification. The study found changes in the concentrations of several proteins that may relate to immune responses and inflammation caused by the disease.
Research Methodology
- The study utilized mass spectrometry-based proteomics. This technology has become significant in protein identification due to its ability to detect a large number of identifications in samples.
- The researchers compared the proteomes or sets of proteins in the sera (blood component) between two groups of Thoroughbred horses – those who are healthy and those suffering from respiratory disease associated with transport (RDT).
Key Findings
- There was a change in levels of certain proteins in the Thoroughbred horses afflicted with RDT.
- Four specific proteins (apolipoprotein F, lipopolysaccharide binding protein, lysozyme, and protein S100-A8) were upregulated (their concentrations were increased) in the sera of the RDT horses.
- On the other hand, keratin 1 protein was downregulated (its concentration was decreased) in the same group of horses.
Research Implications
- The study assumes that these changes in protein levels are connected to the bodies’ inflammation and immune responses as a reaction to the respiratory disease caused by transportation.
- The researchers propose that these proteins could be helpful in understanding more about the development process of RDT.
- The findings open potential paths for the discovery of biomarkers, which are objective indicators of medical state observed from outside the patient, which could indicate the presence of RDT early
This study contributes valuable findings to veterinary and equine medical research, and may motivate future research into developing effective diagnostics or treatment methods for RDT in horses based on these potential biomarker proteins.
Cite This Article
APA
Minamijima Y, Niwa H, Uchida E, Yamamoto K.
(2021).
Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.
J Equine Sci, 32(1), 11-15.
https://doi.org/10.1294/jes.32.11 Publication
Researcher Affiliations
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
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