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Home / Equine Research Bank / Parasitol Res / Comparison of the sensitivity of coprological methods in det...
Parasitology research2014; 113(6); 2401-2406; doi: 10.1007/s00436-014-3919-4

Comparison of the sensitivity of coprological methods in detecting Anoplocephala perfoliata invasions.

Abstract: The autopsy of 487 slaughter horses revealed the presence of Anoplocephala perfoliata in 36 animals. The invasions varied in the intensity (3 to 2,069 tapeworms) and in the level of tapeworms' proglottid maturity. Twenty nine horses were found to contain tapeworms with gravid proglottid. Fecal samples collected from the rectum were tested using following techniques: flotation with solution-saturated NaCl, decantation, McMaster's, and modified sedimentation-flotation methods (50 g feces samples, flotation solution-saturated NaCl and sucrose, specific gravity 1.25 g/ml). The number of A. perfoliata positive fecal samples was significantly higher using the sedimentation-flotation methods 21 (58.33%) than flotation 6 (16.66%), decantation 3 (8.33%), and McMaster's 1 (2.77%) techniques. The sensitivities of the coprological methods during the patent period were 20.69, 10.34, 3.45, and 72.41% for the flotation, decantation, McMaster's, and sedimentation-flotation method, respectively. Sedimentation-flotation techniques proved to be more sensitive than other one. The lowest intensity of invasion possible to detect using this method was nine tapeworms with gravid proglottid.
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Publication Date: 2014-04-29 PubMed ID: 24777342PubMed Central: PMC4031382DOI: 10.1007/s00436-014-3919-4Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the effectiveness of different diagnostic tests for detecting the presence of Anoplocephala perfoliata (a type of tapeworm) in horses. The study found that the sedimentation-flotation method was the most sensitive technique for identifying these parasitic invasions.

Study Overview

The research involved the autopsy of 487 slaughter horses, of which 36 were found to be infected with Anoplocephala perfoliata. The infection levels varied significantly, ranging from 3 to 2,069 tapeworms per horse. Upon investigation, the researchers determined that twenty-nine of the infected horses had tapeworms with mature proglottid, a segment of a tapeworm containing fertilized eggs.

Comparison of Diagnostic Techniques

Various diagnostic methods were used in the study, including:

  • Flotation with solution-saturated NaCl
  • Decantation
  • McMaster’s technique
  • Modified sedimentation-flotation method

The researchers tested fecal samples from the horses using these techniques to determine the presence and number of A. perfoliata. The results of the tests revealed significant differences in sensitivity between the methods.

Results

The accuracy of the diagnostic techniques varied considerably:

  • The sedimentation-flotation method detected the presence of A. perfoliata in 21 of the samples (58.33%).
  • The flotation technique identified the parasite in 6 samples (16.66%).
  • Decantation found the tapeworms in 3 samples (8.33%).
  • McMaster’s method detected them in only 1 sample (2.77%).

Furthermore, the sensitivity of the methods during the patent period (when the tapeworms are mature and releasing eggs) was 20.69% for flotation, 10.34% for decantation, 3.45% for McMaster’s, and 72.41% for the sedimentation-flotation method.

The modified sedimentation-flotation method proved to be the most sensitive technique, capable of detecting an invasion as low as nine tapeworms with gravid proglottid.

Cite This Article

APA
Tomczuk K, Kostro K, Szczepaniak KO, Grzybek M, Studzińska M, Demkowska-Kutrzepa M, Roczeń-Karczmarz M. (2014). Comparison of the sensitivity of coprological methods in detecting Anoplocephala perfoliata invasions. Parasitol Res, 113(6), 2401-2406. https://doi.org/10.1007/s00436-014-3919-4

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 113
Issue: 6
Pages: 2401-2406

Researcher Affiliations

Tomczuk, Krzysztof
  • University of Life Sciences in Lublin, Lublin, Poland, krzysztof.tomczuk@up.lublin.pl.
Kostro, Krzysztof
    Szczepaniak, Klaudiusz Oktawian
      Grzybek, Maciej
        Studzińska, Maria
          Demkowska-Kutrzepa, Marta
            Roczeń-Karczmarz, Monika

              MeSH Terms

              • Animals
              • Cestoda / isolation & purification
              • Cestode Infections / diagnosis
              • Cestode Infections / parasitology
              • Cestode Infections / veterinary
              • Feces / parasitology
              • Horse Diseases / diagnosis
              • Horse Diseases / parasitology
              • Horses
              • Sensitivity and Specificity

              References

              This article includes 39 references
              1. Barclay WP, Phillips TN, Foerner JJ. Intussusception associated with Anoplocephala perfoliata infection in five horses.. J Am Vet Med Assoc 1982;180:752–753.
                pubmed: 7085455
              2. De Almeida JC, Augusto Mde M, Da Silva TG, De Toledo PS, De Souza DF, Antunes J, Molento MB. Quantitative prevalence of Anoplocephala sp. and egg shedding consistency in horses.. Rev Bras Parasitol Vet 2008;17:158–162.
                pubmed: 20059837
              3. Deplazes P, Eckert J, Von Samson-Himmelstjerna G, Zahner H. Lehrbuch der Parasitologie für Die Tiermedizin.. Stuttgart: Enke Verlag; 2013.
                pubmed: 23434846
              4. Drogemuller M, Beelitzb P, Pfisterb K, Schnieder T, Von Samson-Himmelstjernaa G. Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods.. Vet Parasitol 2004;124:205–215.
                doi: 10.1016/j.vetpar.2004.07.012pubmed: 15381301google scholar: lookup
              5. Gasser RB, Williamson RMC, Beveridge I. Anoplocephala perfoliata of horses—significant scope for further research, improved diagnosis and control.. Parasitology 2005;131:1–13.
                doi: 10.1017/S0031182004007127pubmed: 16038391google scholar: lookup
              6. Gawor J. The prevalence and abundance of internal parasites in working horses autopsied in Poland.. Vet Parasitol 1995;58:99–108.
                doi: 10.1016/0304-4017(94)00698-Cpubmed: 7676606google scholar: lookup
              7. Gundlach JL, Sadzikowski AB, Tomczuk K. Diagnosis of tapeworm infestation in horses.. Medycyna Wet 2003;59:532–535.
              8. Gundlach JL, Sadzikowski AB, Tomczuk K, Studzińska MB. Parasites of the alimentary tract of horses from the Lublin district in the light of coproscopic and gross anatomopatological examinations.. Medycyna Wet 2004;60:1089–1092.
              9. Hearn FPD, Hearn EE. A simple diagnostic technique to better determine the prevalence of tapeworms.. J Equine Vet Sci 1995;15:96–98.
                doi: 10.1016/S0737-0806(07)80430-8google scholar: lookup
              10. Hinney B, Wirtherle NC, Kyule M, Miethe N, Zessin KH, Clausen PH. Prevalence of helminths in horses in the state of Brandenburg, Germany.. Parasitol Res 2011;108:1083–1091.
                doi: 10.1007/s00436-011-2362-zpubmed: 21472400google scholar: lookup
              11. Höglund J, Ljungström BL, Nilsson O, Uggla A. Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Anoplocephala perfoliata in horse sera.. Vet Parasitol 1995;59:97–106.
                doi: 10.1016/0304-4017(94)00755-2pubmed: 7483241google scholar: lookup
              12. Kania SA, Reinemeyer CR. Anoplocephala perfoliata coproantigen detection: a preliminary study.. Vet Parasitol 2005;127:115–119.
                doi: 10.1016/j.vetpar.2004.10.003pubmed: 15631904google scholar: lookup
              13. Kjaer LN, Lungholt MM, Nielsen MK, Olsen SN, Maddox-Hyttel C. Interpretation of serum antibody response to Anoplocephala perfoliata in relation to parasite burden and faecal egg count.. Equine Vet J 2007;39:529–533.
                doi: 10.2746/042516407X217876pubmed: 18065311google scholar: lookup
              14. Kornaś S, Skalska M, Nowosad B, Gawor J, Labaziewicz I, Babiuch A. Occurrence of tapeworm, roundworm and botfly larvae in horses from southern Poland.. Medycyna Wet 2007;63:1373–1376.
              15. Kornaś S, Cabaret J, Skalska M, Nowosad B. Horse infection with intestinal helminths in relation to age, sex, access to grass and farm system.. Vet Parasitol 2010;15:285–291.
                doi: 10.1016/j.vetpar.2010.09.007pubmed: 20933334google scholar: lookup
              16. Meana A, Luzon M, Corchero J, Gómez-Bautista M. Reliability of coprological diagnosis of Anoplocephala perfoliata infection.. Vet Parasitol 1998;74:79–83.
                doi: 10.1016/S0304-4017(97)00145-3pubmed: 9493312google scholar: lookup
              17. Michela C, Gianluca F, Rondolottp A, Pellegrini D, Tambinp P, Ming K, Traversa D, Perruccp S. Infestazione da anoplocefalidi (Cestoda, Cyclophyllidea) in cavalliallevati in Toscana.. Ippologia 2009;20:33–38.
              18. Nilsson O, Ljungström BL, Höglund J, Lundquist H, Uggla A. Anoplocephala perfoliata in horses in Sweden: prevalence, infection levels and intestinal lesions.. Acta Vet Scand 1995;36:319–328.
                pmc: PMC8095443pubmed: 7502948
              19. Pavone S, Veronesi F, Genchi C, Fioretti DP, Brianti E, Mandara MT. Pathological changes caused by Anoplocephala perfoliata in the mucosa/submucosa and in the enteric nervous system of equine ileocecal junction.. Vet Parasitol 2011;176:43–52.
                doi: 10.1016/j.vetpar.2010.10.041pubmed: 21075534google scholar: lookup
              20. Proudman CJ, Edwards GB. Validation of a centrifugation/flotation technique for the diagnosis of equine cestodiasis.. Vet Rec 1992;131:71–72.
                doi: 10.1136/vr.131.4.71pubmed: 1529504google scholar: lookup
              21. Proudman CJ, Holdstock NB. Investigation of an outbreak of tapeworm-associated colic in a training yard.. Equine Vet J Suppl 2000;32:37–41.
                doi: 10.1111/j.2042-3306.2000.tb05332.xpubmed: 11202380google scholar: lookup
              22. Proudman CJ, Trees AJ. Tapeworms as a cause of intestinal disease in horses.. Parasitol Today 1999;15:156–159.
                doi: 10.1016/S0169-4758(99)01416-7pubmed: 10322338google scholar: lookup
              23. Raether W, Hänel H. Epidemiology, clinical manifestations and diagnosis of zoonotic cestode infections: an update.. Parasitol Res 2003;91:412–438.
                doi: 10.1007/s00436-003-0903-9pubmed: 13680371google scholar: lookup
              24. Rehbein S, Visser M, Winter R. Koproskopische untersuchungen bei pferden in Deutschland und Österreich.. Pferdeheilkunde 2002;18:439–442.
              25. Rehbein S, Lindner T, Visser M, Winter R. Evaluation of a double centrifugation technique for the detection of Anoplocephala eggs in horse faeces.. J Helminthol 2011;85:409–414.
                doi: 10.1017/S0022149X10000751pubmed: 21138608google scholar: lookup
              26. Rehbein S, Visser M, Winter R. Prevalence, intensity and seasonality of gastrointestinal parasites in abattoir horses in Germany.. Parasitol Res 2013;112:407–413.
                doi: 10.1007/s00436-012-3150-0pubmed: 23052780google scholar: lookup
              27. Rohlf F, Sokal RR. Statistical tables.. San Francisco: WH Freeman and Company; 1995.
              28. Ryu SH, Bak UB, Kim JG, Yoon HJ, Seo HS, Kim JT, Park JY, Lee CW. Cecal rupture by Anoplocephala perfoliata infection in a thoroughbred horse in Seoul Race Park, South Korea.. J Vet Scie (Suwon-si, Korea) 2001;2:189–193.
                pubmed: 12441687
              29. Sadzikowski AB, Studzińska MB, Tomczuk K, Demkowska M. Fasciola hepatica invasion in horses from central and eastern Poland.. Medycyna Wet 2009;65:707–710.
              30. Sangioni LA, Vogel FSF, Da Rosa VA, Camillo G, Araújo L, Montemezzo E, Cezar AS. Epidemiologia da infecção por anoplocefalídeos em eqüinos do Jockey Club de Santa Maria.. Sem Cienc Agr 2009;30:163–170.
              31. Schnieder T. Veterinärmedizinische parasitologie.. Stuttgart: Parey Verlag; 2006.
              32. Schuster R. Morphometric analysis of an Anoplocephala perfoliata population.. Angewandte Parasitol 1991;32:105–111.
                pubmed: 1888072
              33. Skotarek SL, Colwell DD, Goater CP. Evaluation of diagnostic techniques for Anoplocephala perfoliata in horses from Alberta, Canada.. Vet Parasitol 2010;172:249–255.
                doi: 10.1016/j.vetpar.2010.05.005pubmed: 20605685google scholar: lookup
              34. Ślivińska K, Gawor J, Jaworski Z. Gastro-intestinal parasites in yearlings of wild Polish primitive horses from the Popielno Forest Reserve, Poland.. Helminthologia 2009;46:9–13.
                doi: 10.2478/s11687-009-0002-2google scholar: lookup
              35. Slocombe D. A modified critical test and its use in two dose titration trials to assess efficacy of praziquantel for Anoplocephala perfoliata in equids.. Vet Parasitol 2006;136:127–135.
                doi: 10.1016/j.vetpar.2005.10.025pubmed: 16309843google scholar: lookup
              36. Tomczuk K. The characteristic of the Anoplocephala perfoliata invasion in horses in relation to molecular and proteomic aspects of invasion.. Lublin: WUP; 2012.
              37. Traversa D, Fichi G, Campigli M, Rondolotti A, Iorio R, Proudman CJ, Pellegrini D, Perrucci S. A comparison of coprological, serological and molecular methods for the diagnosis of horse infection with Anoplocephala perfoliata (Cestoda, Cyclophyllidea). Vet Parasitol 2008;152:271–277.
                doi: 10.1016/j.vetpar.2007.12.032pubmed: 18280660google scholar: lookup
              38. Trotz-Williams L, Physick-Sheard P, McFarlane H, Pearl DL, Martin SW, Peregrine AS. Occurrence of Anoplocephala perfoliata infection in horses in Ontario, Canada and associations with colic and management practices.. Vet Parasitol 2008;153:73–84.
                doi: 10.1016/j.vetpar.2008.01.016pubmed: 18339482google scholar: lookup
              39. Williamson RM, Beveridge I, Gasser RB. Coprological methods for the diagnosis of Anoplocephala perfoliata infection of the horse.. Aust Vet J 1998;76:618–621.
                doi: 10.1111/j.1751-0813.1998.tb10242.xpubmed: 9791714google scholar: lookup
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