Comparison of thoroughbred and Arabian horses using RAPD markers.
Abstract: We compared pools of DNA from 10 Thoroughbred horses and 10 Arabian horses for the presence of randomly amplified polymorphic DNA (RAPD) markers which might be useful in distinguishing between the breeds. Using 212 decamer oligonucleotides and our polymerase chain reaction (PCR) conditions, 173 of the primers produced scoreable bands. The number of bands ranged from 0 to 9 with an average of 3.6. In family studies using 11 arbitrarily selected primers, five of the 11 primers produced polymorphic bands which exhibited Mendelian inheritance as dominant markers. When comparing the pooled DNA from Thoroughbred and Arabian horses we found 10 primers which identified markers present in the pooled DNA from one breed but absent in the pool from the other breed. Testing individual horses revealed that only two markers were wholly absent for one group while being present among members of the other. Primer UBC-85 (5'-GTGCTCGTGC-3') detected a pair of markers absent in Thoroughbred horses but present among 11 of 31 Arabian horses. These markers were 1500 and 1700 base pairs (bp) long and designated UBC-85C and UBC-85D, respectively. Primer UBC-126 (5'-CTTTCGTGCT-3') detected a 1000 bp marker (designated UBC-126C) absent in 20 of 20 Thoroughbred horses but present in 31 of 31 Arabian horses.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1994-06-01 PubMed ID: 7943977DOI: 10.1111/j.1365-2052.1994.tb00414.xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research compares DNA samples from Thoroughbred and Arabian horses to determine distinctive genetic traits between the breeds. It was found that certain genetic markers were present in one breed, but absent in the other, potentially aiding in breed identification.
Methodology
- The research team started with pools of DNA from 10 Thoroughbred horses and 10 Arabian horses.
- They then looked for the presence of randomly amplified polymorphic DNA (RAPD) markers. These markers are portions of DNA that can help distinguish genetic variations between the two breeds.
- Using 212 decamer oligonucleotides and standardized polymerase chain reaction (PCR) conditions, they were able to produce results from 173 of the primers. The number of scoreable bands they obtained ranged anywhere from 0 to 9, with the average being 3.6.
Family Studies
- The team started family studies using 11 arbitrarily selected primers.
- Of those 11 primers, five produced polymorphic bands demonstrating Mendelian inheritance as dominant markers. Mendelian inheritance refers to the way genes and traits are passed down from parents to offspring, hypothesizing that these genes have a dominant or recessive pattern.
Comparison of Breeds
- When the researchers compared the pooled DNA samples, 10 primers helped identify markers present in DNA from one breed but not in the other.
- However, when testing individual horses, only two markers were found to be completely absent in one breed and completely present in the other.
- Primer UBC-85 detected a pair of markers that were absent in Thoroughbred horses but present in some Arabian horses. These markers were 1500 and 1700 base pairs long and were designated as UBC-85C and UBC-85D, respectively.
- Another primer, UBC-126, identified a 1000 base pair long marker, named UBC-126C, that was absent in all the tested Thoroughbred horses but present in all the tested Arabian horses.
Findings
- The presence or absence of specific markers, as indicated by the primer tools, could serve as distinctive genetic traits between the Thoroughbred and Arabian horse breeds, potentially aiding breed identification and contributing to the study of horse genetics.
Cite This Article
APA
Bailey E, Lear TL.
(1994).
Comparison of thoroughbred and Arabian horses using RAPD markers.
Anim Genet, 25 Suppl 1, 105-108.
https://doi.org/10.1111/j.1365-2052.1994.tb00414.x Publication
Researcher Affiliations
- Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
MeSH Terms
- Animals
- Base Sequence
- DNA / genetics
- DNA Primers / genetics
- Female
- Gene Amplification
- Gene Frequency
- Genetic Markers
- Horses / genetics
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Species Specificity
Citations
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