Comparison of ticarcillin and piperacillin in Kenney’s semen extender.
Abstract: Ticarcillin and piperacillin were compared to determine their effect on sperm motility and bacterial growth of equine semen samples diluted in Kenney's glucose skim milk semen extender. Each ejaculate (n=11) was divided into three portions and glucose skim milk semen extender solution was added. The control semen extender solution contained extended semen and no antibiotic, whereas ticarcillin and piperacillin solutions contained extended semen plus 1.0mg/mL of ticarcillin or piperacillin, respectively. An aliquot was removed (1h after collection) to evaluate sperm motility and microbial concentration. All three solutions were stored at 4 degrees C and aliquots were obtained at 24 and 48 h to determine sperm motility and microbial concentration. Mean percentages of motile and progressively motile sperm did not differ significantly among control and antibiotic-containing solutions after storage. Control-extended semen samples from ejaculates of stallions (n=11) were contaminated with aerobic gram-positive and gram-negative bacteria. In solutions that contained either antibiotic, growth of these microbes was inhibited after 1, 24, and 48 h at 4 degrees C. Semen samples from stallions (n=5) were extended with Kenney's glucose skim milk extender containing no antibiotic, ticarcillin or piperacillin and then inoculated with approximately 5 x 10(2)CFU/mL Klebsiella pneumoniae or Pseudomonas aeruginosa; there was no significant difference between antibiotics in the inhibition of microbial growth. In conclusion, piperacillin was an appropriate alternative to ticarcillin in extenders for equine semen.
Publication Date: 2007-08-15 PubMed ID: 17706757PubMed Central: PMC2128061DOI: 10.1016/j.theriogenology.2007.03.031Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- N.I.H.
- Extramural
Summary
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The research investigates the effects of the antibiotics ticarcillin and piperacillin on the motility and bacterial growth of horse semen extended in Kenney’s glucose skim milk extender. The study found that there’s no significant difference in sperm motility between using ticarcillin, piperacillin or no antibiotic. However, both antibiotics effectively inhibited bacterial growth. Piperacillin was concluded to be a suitable alternative to ticarcillin for extending equine semen.
Research Methodology
- The study used 11 ejaculates, equally divided into three groups and diluted in Kenney’s glucose skim milk semen extender. The groups consisted of a control group (with no antibiotic), a ticarcillin group (semen plus 1.0mg/mL of ticarcillin) and a piperacillin group (semen plus 1.0mg/mL of piperacillin).
- An aliquot (a portion of the mixture) was removed from each solution one hour after collection to assess sperm motility and microbial concentration.
- All three solutions were then stored at 4°C, and additional samples were taken at 24 and 48 hours for assessment of sperm motility and microbial concentration.
Findings & Interpretation
- The research found no significant difference in the mean percentages of motile and progressively motile sperm among the control and antibiotic-containing solutions after storage.
- In the control-extended semen samples, evidence of contamination from both aerobic gram-positive and gram-negative bacteria was found. However, in solutions with either antibiotic, there was effective inhibition of these microbes after 1, 24, and 48 hours at 4 degrees C.
- The semen extender solutions with ticarcillin or piperacillin were additionally tested with inoculations of Klebsiella pneumoniae and Pseudomonas aeruginosa. The results showed no significant difference between the two antibiotics in their capacity to inhibit microbial growth.
Conclusion
- From these findings, it can be concluded that piperacillin is an appropriate alternative to ticarcillin for use in equine semen extenders. Both antibiotics showed a similar ability to inhibit bacterial growth without negatively affecting sperm motility.
Cite This Article
APA
Dietz JP, Sertich PL, Boston RC, Benson CE.
(2007).
Comparison of ticarcillin and piperacillin in Kenney’s semen extender.
Theriogenology, 68(6), 848-852.
https://doi.org/10.1016/j.theriogenology.2007.03.031 Publication
Researcher Affiliations
- Department of Clinical Studies, Section of Reproduction, 382 West Street Road, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Horses
- Male
- Microbial Sensitivity Tests
- Organ Preservation Solutions / pharmacology
- Piperacillin / pharmacology
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / drug effects
- Spermatozoa / drug effects
- Spermatozoa / microbiology
- Ticarcillin / pharmacology
Grant Funding
- T35 RR007065 / NCRR NIH HHS
- T35 RR007065-10 / NCRR NIH HHS
References
This article includes 10 references
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