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Home / Equine Research Bank / Infect Immun / Complement requirement for virus neutralization by antibody ...
Infection and immunity1981; 31(2); 636-640; doi: 10.1128/iai.31.2.636-640.1981

Complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection.

Abstract: Pony foals, negative for detectable serum-neutralizing antibody to equine herpesvirus 1 by the standard tube-culture virus neutralization test, were experimentally infected with equine herpesvirus 1. Complement-requiring (CR) and non-complement-requiring (NCR) serum-neutralizing antibodies were evaluated in preinfection and postinfection sera by means of a complement-enhanced plaque reduction assay. Low levels of CR antibodies were found in the preinfection sera of only group II ponies. Upon infection, CR antibodies were detected by day 2 postinfection and reached peak titers between 7 and 14 days postinfection in the antisera of all ponies. NCR antibodies were detected later than CR antibodies and at levels approximately 40 to 150 times lower than the latter. CR/NCR ratios indicated that complement requirement was greatest early in the acute stages of disease and that this requirement decreased during the convalescent phase. Fractionation of 1-week and 2-week postinfection antisera of group I ponies indicated the CR antibody activity resided in both the 7S and 19S fractions. Total serum complement levels of the ponies were quantified throughout the infection with an equine anti-goat erythrocyte hemolytic system. In vivo, complement levels were depressed for all ponies during the first 2 weeks of infection. A decline in complement levels was seen as early as day 2, and they decreased to an average of 35% of preinfection levels on day 10 postinfection for all ponies.
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Publication Date: 1981-02-01 PubMed ID: 6260672PubMed Central: PMC351356DOI: 10.1128/iai.31.2.636-640.1981Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how antibodies requiring, or not requiring, complement proteins react before and after infection with equine herpesvirus 1 in pony foals. The results show a drop in serum complement levels during infection.

Methods and Experimentation

  • Pony foals without detectable antibodies against equine herpesvirus 1, were infected with the same virus for this experiment.
  • Two types of serum-neutralizing antibodies were studied – Complement-requiring (CR) and non-complement-requiring (NCR).
  • A complement-enhanced plaque reduction assay was utilized to evaluate these antibodies in preinfection and postinfection sera.

Results and Findings

  • Before the infection (pre-infection sera), low levels of CR antibodies were found, but only in group II ponies.
  • Upon infection, CR antibodies were detected by day 2 post-infection and they achieved their peak levels between day 7 and day 14 post-infection in all ponies.
  • NCR antibodies were detected later than CR antibodies, and their levels were notably lower, around 40 to 150 times less.
  • The ratio of CR to NCR antibodies showed that the need for complement proteins was most significant early in the disease’s acute stages, and this requirement decreased during the recovery phase (convalescent phase).
  • When examining the infected ponies’ serum after one week and two weeks, results showed that the CR antibody activity was present in both the 7S and 19S fractions.
  • Furthermore, total serum complement levels were measured throughout the infection period. These levels showed a drop in the first two weeks of infection across all ponies.
  • The decline in complement levels was evident as early as day 2, and by day 10 post-infection, the levels reduced to an average of 35% of preinfection levels for all ponies.

Implications of Research

  • This research provides insights into the immune response of ponies to equine herpesvirus 1 infection. Understanding the action and interaction of the different types of antibodies produced in response to infection can aid in the design of future therapeutic interventions and vaccines.

Cite This Article

APA
Snyder DB, Myrup AC, Dutta SK. (1981). Complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection. Infect Immun, 31(2), 636-640. https://doi.org/10.1128/iai.31.2.636-640.1981

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 31
Issue: 2
Pages: 636-640

Researcher Affiliations

Snyder, D B
    Myrup, A C
      Dutta, S K

        MeSH Terms

        • Animals
        • Antibodies, Viral
        • Complement Activation
        • Herpesviridae / immunology
        • Herpesvirus 1, Equid / immunology
        • Horse Diseases / immunology
        • Horses

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        Citations

        This article has been cited 8 times.
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