Complete genome analysis of Glutamicibacter creatinolyticus from mare abscess and comparative genomics provide insight of diversity and adaptation for Glutamicibacter.
Abstract: Bacteria of the genusGlutamicibacterare considered ubiquitous because they can be found in soil, water and air. They have already been isolated from different habitats, including different types of soil, clinical samples, cheese and plants. Glutamicibacter creatinolyticus is a Gram-positive bacterium important to various biotechnological processes, however, as a pathogen it is associated to urinary tract infections and bacteremia. Recently,Glutamicibacter creatinolyticusLGCM 259 was isolated from a mare, which displayed several diffuse subcutaneous nodules with heavy vascularization. In this study, sequencing, genomic analysis ofG. creatinolyticusLGCM 259 and comparative analyseswere performedamong 4representatives of different members of genusfromdifferent habitats, available in the NCBI database. The LGCM 259 strain's genome carries important factors of bacterial virulence that are essential in cell viability, virulence, and pathogenicity. Genomic islands were predicted for 4 members of genusGlutamicibacter,showing ahigh number of GEIs,which may reflect a high interspecific diversity and a possible adaptive mechanism responsible for the survival of each species in its specific niche. Furthermore,G. creatinolyticusLGCM 259 sharessyntenicregions, albeit with a considerable loss of genes, in relation to the other species. In addition,G. creatinolyticusLGCM 259 presentsresistancegenes to 6 differentclasses ofantibiotics and heavy metals, such as: copper, arsenic, chromium and cobalt-zinc-cadmium.Comparative genomicsanalysescouldcontribute to the identification of mobile genetic elements particular to the speciesG. creatinolyticuscompared to other members of genus. The presence of specific regions inG. creatinolyticuscould be indicative of their rolesin host adaptation, virulence, and the characterization ofastrain that affects animals.
Copyright © 2020 Elsevier B.V. All rights reserved.
Publication Date: 2020-03-17 PubMed ID: 32171826DOI: 10.1016/j.gene.2020.144566Google Scholar: Lookup
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- Journal Article
Summary
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This study investigates the genetic makeup of Glutamicibacter creatinolyticus, a bacteria found in diverse locations and associated with both biotechnological uses and infections. The research particularly studies a strain of this bacteria found in a mare, analyzing its full genome and comparing it with other strains to better understand its diversity and adaptability.
Study Introduction and Background
- The Glutamicibacter genus refers to bacteria that can be found in various environments such as soil, water, and air. They have been isolated from various habitats like soil types, clinical samples, cheese, and plants.
- The specific strain of Glutamicibacter creatinolyticus studied in this research was isolated from a mare and is associated with several health conditions including urinary tract infections and bacteremia.
- The bacteria are also known to possess important biotechnological applications.
Analysis Methodology
- Sequencing and genomic analysis were performed on the LGCM 259 strain of G. creatinolyticus. Other strains from different habitats were also studied for a comparative analysis.
- The researchers used the NCBI database as their source for other members of the genus Glutamicibacter. This helped to compare the diversity in the genomes of these different bacteria.
Major Findings
- The LGCM 259 strain was found to possess key factors responsible for bacterial virulence, pathogenicity, and cell viability. These are critical to the capacity to infect other organisms.
- High numbers of Genomic Islands were predicted for the genus Glutamicibacter. These Genomic Islands or GEIs represent high interspecies diversity and possible adaptive mechanisms that each species uses to survive in specific niches.
- The LGCM 259 strain shared synteny – a similar order of genes – with other Glutamicibacter species, but with a significant loss of genes.
- The LGCM 259 strain also showed the presence of resistance genes to six different classes of antibiotics and the heavy metals copper, arsenic, chromium, and cobalt-zinc-cadmium.
Conclusions and Implications
- Comparative genomic analysis helped to identify mobile genetic elements specifically belonging to the species G. creatinolyticus, compared to other Glutamicibacter genus members.
- This study indicates that specific regions in G. creatinolyticus may play a role in host adaptation and virulence, thereby enabling this strain to impact animals.
- The findings lay the groundwork for better understanding this bacterium and potentially mitigating its impact on health, while harnessing its beneficial biotechnological applications.
Cite This Article
APA
Santos RG, Hurtado R, Gomes LGR, Profeta R, Rifici C, Attili AR, Spier SJ, Mazzullo G, Morais-Rodrigues F, Gomide ACP, Brenig B, Gala-García A, Cuteri V, Castro TLP, Ghosh P, Seyffert N, Azevedo V.
(2020).
Complete genome analysis of Glutamicibacter creatinolyticus from mare abscess and comparative genomics provide insight of diversity and adaptation for Glutamicibacter.
Gene, 741, 144566.
https://doi.org/10.1016/j.gene.2020.144566 Publication
Researcher Affiliations
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: roselanegr@gmail.com.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Department of Veterinary Science, University of Messina (Italy), Polo Universitario, dell'Annunziata, 98168 Messina, ME, Italy.
- School of Biosciences and Veterinary Medicine, University of Camerino (Italy), Via Circonvallazione 93/95, 62024 Matelica, MC, Italy. Electronic address: annarita.attili@unicam.it.
- Department of Veterinary Medicine and Epidemiology, University of California, Davis, CA, USA. Electronic address: sjspier@ucdavis.edu.
- Department of Veterinary Science, University of Messina (Italy), Polo Universitario, dell'Annunziata, 98168 Messina, ME, Italy. Electronic address: giuseppe.mazzullo@unime.it.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, Göttingen, Germany. Electronic address: bbrenig@gwdg.de.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Institute of Biological Sciences, Federal University of Para, PA, Brazil.
- School of Biosciences and Veterinary Medicine, University of Camerino (Italy), Via Circonvallazione 93/95, 62024 Matelica, MC, Italy. Electronic address: vincenzo.cuteri@unicam.it.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil.
- Department of Computer Science, Virginia Commonwealth University, Richmond, VA 23284, USA.
- Institute of Biology, Federal University of Bahia, Salvador, BA, Brazil.
- Cellular and Molecular Genetics Laboratory, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
MeSH Terms
- Abscess / genetics
- Abscess / microbiology
- Abscess / veterinary
- Adaptation, Physiological / genetics
- Animals
- Genetic Variation
- Genome, Bacterial
- Genomic Islands / genetics
- Genomics
- Horses / microbiology
- Male
- Micrococcaceae / genetics
- Micrococcaceae / pathogenicity
- Phylogeny
- Virulence / genetics
Conflict of Interest Statement
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Citations
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- Lukianova AA, Evseev PV, Stakheev AA, Kotova IB, Zavriev SK, Ignatov AN, Miroshnikov KA. Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence. Plants (Basel) 2021 Feb 13;10(2).
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