Complete Genome Sequences of Getah Virus Strains Isolated from Horses in 2016 in Japan.
Abstract: Getah virus is mosquito-borne and causes disease in horses and pigs. We sequenced and analyzed the complete genomes of three strains isolated from horses in Ibaraki Prefecture, eastern Japan, in 2016. They were almost identical to the genomes of strains recently isolated from horses, pigs, and mosquitoes in Japan.
Copyright © 2017 Nemoto et al.
Publication Date: 2017-08-03 PubMed ID: 28774985PubMed Central: PMC5543647DOI: 10.1128/genomeA.00750-17Google Scholar: Lookup
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- Journal Article
Summary
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This research involves the investigation and analysis of the complete genomes of Getah virus strains isolated from horses in Japan in 2016, revealing a strong similarity to recent strains found in the same country.
Overview of the Research
- This study focuses on Getah virus, a mosquito-borne pathogen that causes diseases in horses and pigs. The virus is especially prevalent in the country of Japan.
- Researchers sought to understand the virus better by sequencing and analyzing the complete genomes of three strains that were isolated from horses in Ibaraki Prefecture, a region in eastern Japan, in 2016.
Genome Sequencing and Analysis
- The genomes of the selected virus strains were subject to complete sequencing. This process involves determining the precise order of nucleotides within a DNA molecule, providing a comprehensive genetic map of the virus.
- Following sequencing, the isolated genomes were then analyzed in depth. Analysis likely included comparison to known viral genome structures, identification of unique or notable genetic features, and comparison between the strains.
Comparison to Recent Strains
- Findings from the study showed that the genomes of the three strains studied were almost identical to the genomes of strains recently isolated from horses, pigs, and mosquitoes in Japan.
- This suggests a relative lack of significant variation in Getah virus strains within recent years, at least within the studied physical and temporal scope. The virus appears to have remained largely stable over time and consistent across host species.
Significance of the Research
- By generating complete genomes of Getah virus strains, the research significantly contributes to the scientific understanding of this particular virus. It gives further insight into its genetic makeup, which aids in tracking its evolution, studying its pathogenicity, and developing methods for control and prevention.
- The findings of this study may also stimulate further research endeavors. The genetic stability observed over time and across hosts remains an interesting aspect to explore, which may uncover why the Getah virus has remained similarly virulent over time and could provide clues to its future evolution.
Cite This Article
APA
Nemoto M, Bannai H, Ochi A, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kokado H, Kondo T.
(2017).
Complete Genome Sequences of Getah Virus Strains Isolated from Horses in 2016 in Japan.
Genome Announc, 5(31), e00750-17.
https://doi.org/10.1128/genomeA.00750-17 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, Tochigi, Japan nemoto_manabu@equinst.go.jp.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Thermo Fisher Scientific, Life Technologies Japan Ltd., Tokyo, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi, Japan.
References
This article includes 11 references
- Fukunaga Y, Kumanomido T, Kamada M. Getah virus as an equine pathogen.. Vet Clin North Am Equine Pract 2000 Dec;16(3):605-17.
- Izumida A, Takuma H, Inagaki S, Kubota M, Hirahara T, Kodama K, Sasaki N. Experimental infection of Getah virus in swine.. Nihon Juigaku Zasshi 1988 Jun;50(3):679-84.
- Yago K, Hagiwara S, Kawamura H, Narita M. A fatal case in newborn piglets with Getah virus infection: isolation of the virus.. Nihon Juigaku Zasshi 1987 Dec;49(6):989-94.
- Kamada M, Ando Y, Fukunaga Y, Kumanomido T, Imagawa H, Wada R, Akiyama Y. Equine Getah virus infection: isolation of the virus from racehorses during an enzootic in Japan.. Am J Trop Med Hyg 1980 Sep;29(5):984-8.
- Bannai H, Ochi A, Nemoto M, Tsujimura K, Yamanaka T, Kondo T. A 2015 outbreak of Getah virus infection occurring among Japanese racehorses sequentially to an outbreak in 2014 at the same site.. BMC Vet Res 2016 Jun 10;12:98.
- Nemoto M, Bannai H, Tsujimura K, Kobayashi M, Kikuchi T, Yamanaka T, Kondo T. Getah Virus Infection among Racehorses, Japan, 2014.. Emerg Infect Dis 2015 May;21(5):883-5.
- Kobayashi D, Isawa H, Ejiri H, Sasaki T, Sunahara T, Futami K, Tsuda Y, Katayama Y, Mizutani T, Minakawa N, Ohta N, Sawabe K. Complete Genome Sequencing and Phylogenetic Analysis of a Getah Virus Strain (Genus Alphavirus, Family Togaviridae) Isolated from Culex tritaeniorhynchus Mosquitoes in Nagasaki, Japan in 2012.. Vector Borne Zoonotic Dis 2016 Dec;16(12):769-776.
- Bannai H, Nemoto M, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kondo T. Geospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015.. BMC Vet Res 2017 Jun 19;13(1):187.
- Wekesa SN, Inoshima Y, Murakami K, Sentsui H. Genomic analysis of some Japanese isolates of Getah virus.. Vet Microbiol 2001 Nov 8;83(2):137-46.
- Bannai H, Nemoto M, Ochi A, Kikuchi T, Kobayashi M, Tsujimura K, Yamanaka T, Kondo T. Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014.. J Clin Microbiol 2015 Jul;53(7):2286-91.
- Nemoto M, Bannai H, Tsujimura K, Yamanaka T, Kondo T. Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan.. Arch Virol 2016 Jun;161(6):1691-5.
Citations
This article has been cited 3 times.- Ochi A, Bannai H, Aonuma H, Kanuka H, Uchida-Fujii E, Kinoshita Y, Ohta M, Kambayashi Y, Tsujimura K, Ueno T, Nemoto M. Surveillance of Getah virus in mosquitoes and racehorses from 2016 to 2019 at a training center in Ibaraki Prefecture, Japan, a site of several previous Getah virus outbreaks.. Arch Virol 2023 Jan 7;168(2):35.
- Takeishi M, Kuwata R, Ono T, Sasaki A, Ogata M, Iwata E, Taji S, Koike M, Nemoto M, Bannai H, Isawa H, Maeda K, Morikawa S, Kitagawa H, Yoshikawa Y. Seroconversion of anti-Getah virus antibody among Japanese native Noma horses around 2012.. J Vet Med Sci 2022 Nov 18;84(12):1605-1609.
- Ren T, Mo Q, Wang Y, Wang H, Nong Z, Wang J, Niu C, Liu C, Chen Y, Ouyang K, Huang W, Wei Z. Emergence and Phylogenetic Analysis of a Getah Virus Isolated in Southern China.. Front Vet Sci 2020;7:552517.
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