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Archives of virology2020; 165(7); 1715-1717; doi: 10.1007/s00705-020-04647-x

Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes.

Abstract: Venezuelan equine encephalitis virus (VEEV) is an important pathogen of medical and veterinary importance in the Americas. In this report, we present the complete genome sequences of five VEEV isolates obtained from pools of Culex (Melanoconion) gnomatos (4) or Culex (Melanoconion) pedroi (1) from Iquitos, Peru. Genetic and phylogenetic analyses showed that all five isolates grouped within the VEEV complex sister to VEEV IIIC and are members of subtype IIID. This is the first report of full-length genomic sequences of VEEV IIID.
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Publication Date: 2020-05-16 PubMed ID: 32417973DOI: 10.1007/s00705-020-04647-xGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research reports the complete genomic sequences of five Venezuelan equine encephalitis virus (VEEV) isolates from mosquitoes in Iquitos, Peru, which fall into the subgroup IIID. This marks the first time the entire genome of VEEV IIID has been sequenced and analyzed.

Objective and Methodology

  • The primary purpose of the research was to acquire and analyze the complete genomic sequences of five Venezuelan equine encephalitis virus (VEEV) isolates. These isolates were all found in a species of mosquito in rural Peru. VEEV is a virus that can cause serious illness in both humans and animals.
  • The VEEV isolates analyzed for this study were obtained from pools of two types of Culex mosquitoes, Culex (Melanoconion) gnomatos and Culex (Melanoconion) pedroi. These mosquitoes commonly inhabit regions of the Americas, where the virus is prevalent.

Findings

  • The researchers performed genetic and phylogenetic analyses of all five isolates and found that they all grouped within the VEEV complex, indicating that they are closely related genetically.
  • The isolates were all found to be members of the subtype IIID, based on their genetic markers and sequences. This subtype is one among several that compose the larger grouping of VEEV viruses.

Significance

  • The report is of particular importance because it marks the first time that the complete genomic sequences of VEEV subtype IIID have been described and documented. Previous studies have not fully sequenced or analyzed this specific subtype.
  • The findings broaden our understanding of the genetic makeup, variability, and evolution of the VEEV virus, particularly within the IIID subtype. This detailed knowledge could aid in vaccine development, early detection, and treatment strategies.

Cite This Article

APA
Padilla SL, Prieto K, Dohm DJ, Turell MJ, Klein TA, Fernández R, Watts DM, Lowen RG, Palacios GF, Pitt ML, Wiley MR, Nasar F. (2020). Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes. Arch Virol, 165(7), 1715-1717. https://doi.org/10.1007/s00705-020-04647-x

Publication

Archives of virology
ISSN: 1432-8798
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 165
Issue: 7
Pages: 1715-1717

Researcher Affiliations

Padilla, Susana L
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
  • Public Health Command-Pacific, Honolulu, Hawaii, USA.
Prieto, Karla
  • Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.
  • College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Dohm, David J
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
Turell, Michael J
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
  • VectorID LLC, Frederick, MD, USA.
Klein, Terry A
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
  • 65th Medical Brigade/Medical Department Activity-Korea, Unit 15281, Pyeongtaek, Republic of Korea.
Fernández, Roberto
  • Department of Entomology, U.S. Naval Medical Research Unit No. 6, Callao, Peru.
Watts, Douglas M
  • Department of Entomology, U.S. Naval Medical Research Unit No. 6, Callao, Peru.
  • Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA.
Lowen, Robert G
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
  • Public Health Command-Atlantic, Fort George G. Meade, MD, USA.
Palacios, Gustavo F
  • Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.
Pitt, Margaret L
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA.
Wiley, Michael R
  • Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.
  • College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Nasar, Farooq
  • Virology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD, 21702, USA. farooq.nasar.ctr@mail.mil.

MeSH Terms

  • Animals
  • Base Sequence
  • Culex / virology
  • Encephalitis Virus, Venezuelan Equine / classification
  • Encephalitis Virus, Venezuelan Equine / genetics
  • Encephalitis Virus, Venezuelan Equine / isolation & purification
  • Encephalomyelitis, Venezuelan Equine / transmission
  • Encephalomyelitis, Venezuelan Equine / virology
  • Genome, Viral
  • Genomics
  • Horses
  • Mosquito Vectors / virology
  • Peru
  • Phylogeny

Grant Funding

  • U0501-17-AF-CS-OC / Military Infectious Diseases Research Program

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This article includes 13 references
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Citations

This article has been cited 1 times.
  1. Costa MM, Guidez A, Briolant S, Talaga S, Issaly J, Naroua H, Carinci R, Gaborit P, Lavergne A, Dusfour I, Duchemin JB, Almeras L. Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling. Trop Med Infect Dis 2023 Mar 13;8(3).
    doi: 10.3390/tropicalmed8030168pubmed: 36977169google scholar: lookup
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