Complete Mitochondrial genome of an equine intestinal parasite, Triodontophorus brevicauda (Chromadorea: Strongylidae): the first characterization within the genus.
Abstract: The complete mitochondrial (mt) genome sequence of Triodontophorus brevicauda, an intestinal equine nematode parasite was determined for the first time. The circular T. brevicauda mt genome is 14,305 bp in length and contains 36 genes, of which 12 code for protein, 22 for transfer RNA, and two for ribosomal RNA, and lacks atp8 mtDNA gene. Phylogenetic analysis based on the concatenated amino acid sequence of the 12 protein-coding genes was performed using three different tree-building methods. The Strongyloidea cluster divides into two large branches, and each nematode family included in our study forms an independent clade, though paraphyly confounds the issue at some nodes. T. brevicauda clusters together with Cylicocyclus insignis with high statistical support. The mtDNA data in this study not only provide a new mtDNA resource for phylogeny, but also become a novel and useful genetic marker for further studies on the identification, population genetics, and molecular epidemiology of the genus Triodontophorus in equine.
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
Publication Date: 2015-06-20 PubMed ID: 26100950DOI: 10.1016/j.parint.2015.06.006Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers have successfully sequenced the complete mitochondrial genome of the equine intestinal parasite, Triodontophorus brevicauda. The study reveals this genome is made up of 36 genes and provides crucial data potentially useful for the identification, population genetics, and molecular epidemiology of this genus.
Research Methodology
- The scientists carried out genome sequencing on T. brevicauda, an intestinal equine nematode parasite. This was the first time the mitochondrial genome of this parasite was completely sequenced.
- The mitochondrial genome in question was found to be a circular one with a length of 14,305 base pairs. It contains a total of 36 genes, 12 of which encode for protein, 22 for transfer RNA, and two for ribosomal RNA.
- The ATP8 mitochondrial DNA gene, typically part of many mitochondrial genomes, was conspicuously absent in T. brevicauda.
- The research team performed a phylogenetic analysis based on the concatenated amino acid sequence derived from the 12 protein-coding genes. These findings were achieved using three different tree-building techniques.
Research Outcomes
- The phylogenetic tree of the Strongyloidea cluster, of which the T. brevicauda is a part, showed two large branches, with each nematode family assessed in the study forming an independent clade.
- Paraphyly created some confusion at certain tree nodes. Paraphyly refers to the condition where some but not all descendants of a common ancestor are included in a taxonomic group.
- It was found that T. brevicauda has a close relationship with Cylicocyclus insignis, as both parasites clustered closely together on the phylogenetic tree.
- The mitochondrial DNA data derived from this study offers a new resource for studying phylogeny – the evolutionary history and relationships among individual species.
- This data also presents itself as a useful genetic marker for future research examining the identification, population genetics, and molecular epidemiology of the Triodontophorus genus in equines.
Cite This Article
APA
Duan H, Gao JF, Hou MR, Zhang Y, Liu ZX, Gao DZ, Guo DH, Yue DM, Su X, Fu X, Wang CR.
(2015).
Complete Mitochondrial genome of an equine intestinal parasite, Triodontophorus brevicauda (Chromadorea: Strongylidae): the first characterization within the genus.
Parasitol Int, 64(5), 429-434.
https://doi.org/10.1016/j.parint.2015.06.006 Publication
Researcher Affiliations
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province 161006, PR China.
- Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province 161006, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
- College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China. Electronic address: chunrenwang@sohu.com.
MeSH Terms
- Animals
- DNA, Helminth / genetics
- Gene Expression Regulation / physiology
- Genome, Mitochondrial
- Helminth Proteins / genetics
- Helminth Proteins / metabolism
- Phylogeny
- RNA, Helminth / genetics
- Strongylida / genetics
Citations
This article has been cited 11 times.- Liu S, Liu Y, Chen B, Lu X, Jiang D, Geng L, Wang X, Peng K, Du C, Ren T, Yang X. The complete mitochondrial genome of Morishitium polonicum (Trematoda, Cyclocoelidae) and its phylogenetic implications. Parasitol Res 2023 Sep 9;.
- Gacad JLJ, Yurlova NI, Ponomareva NM, Urabe M. Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications. Parasitol Res 2023 Jul;122(7):1545-1556.
- Yang H, Chen T, Dong W. Comparative analysis of the mitochondrial genome of Dermacentor steini from different regions in China. Parasitology 2023 Feb;150(2):195-205.
- Chang QC, Fu X, Song CL, Liu HB, Sun Y, Jia N, Jiang JF, Wang CR, Jiang BG. The complete mitochondrial genome of Haemaphysalis concinna (Ixodida: Ixodidae). Mitochondrial DNA B Resour 2018 Mar 14;3(1):348-349.
- Solórzano-García B, Ospina AL, Rondón S, Pérez-Ponce de León G. Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle. Int J Parasitol Parasites Wildl 2020 Apr;11:17-28.
- Gao Y, Zhang Y, Yang X, Qiu JH, Duan H, Xu WW, Chang QC, Wang CR. Mitochondrial DNA Evidence Supports the Hypothesis that Triodontophorus Species Belong to Cyathostominae. Front Microbiol 2017;8:1444.
- Abbas G, Nielsen MK, E-Hage C, Ghafar A, Beveridge I, Bauquier J, Beasley A, Wilkes EJA, Carrigan P, Cudmore L, Jacobson C, Hughes KJ, Jabbar A. Recent advances in intestinal helminth parasites of horses in the Asia-Pacific region: Current trends, challenges and future directions. Int J Parasitol Drugs Drug Resist 2025 Dec;29:100622.
- Jia H, Tang L, Fu Y, Xiong Y, Yan L, Shao C, Li K, Zhang D, Hu D. The first mitogenome of Petrovinema skrjabini from Equus ferus przewalskii: a phylogenetic analysis within the Strongylidae family. Parasit Vectors 2025 Jul 11;18(1):278.
- Chen B, Duan M, Liu S, Liu Y, Tang S, Jiang D, Gu W, Zhang Q, Yang X. The complete mitochondrial genome and phylogenetic implications of Paradoxopsyllus custodis and Stenischia montanis yunlongensis. Sci Rep 2024 Dec 30;14(1):31555.
- Ma L, Li J, He J, Jiang T, Hao Y, Bu Y. Characterization and phylogenetic analysis of the mitochondrial genome of Cylicostephanus longibursatus. Parasitol Res 2024 Oct 29;123(10):363.
- Zeng JL, Chen HX, Ni XF, Kang JY, Li L. Molecular phylogeny of the family Rhabdiasidae (Nematoda: Rhabditida), with morphology, genetic characterization and mitochondrial genomes of Rhabdias kafunata and R. bufonis. Parasit Vectors 2024 Mar 1;17(1):100.
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