Complete molecular genome analyses of equine rotavirus A strains from different continents reveal several novel genotypes and a largely conserved genotype constellation.
Abstract: In this study, the complete genome sequences of seven equine group A rotavirus (RVA) strains (RVA/Horse-tc/GBR/L338/1991/G13P[18], RVA/Horse-wt/IRL/03V04954/2003/G3P[12] and RVA/Horse-wt/IRL/04V2024/2004/G14P[12] from Europe; RVA/Horse-wt/ARG/E30/1993/G3P[12], RVA/Horse-wt/ARG/E403/2006/G14P[12] and RVA/Horse-wt/ARG/E4040/2008/G14P[12] from Argentina; and RVA/Horse-wt/ZAF/EqRV-SA1/2006/G14P[12] from South Africa) were determined. Multiple novel genotypes were identified and genotype numbers were assigned by the Rotavirus Classification Working Group: R9 (VP1), C9 (VP2), N9 (NSP2), T12 (NSP3), E14 (NSP4), and H7 and H11 (NSP5). The genotype constellation of L338 was unique: G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11. The six remaining equine RVA strains showed a largely conserved genotype constellation: G3/G14-P[12]-I2/I6-R2-C2-M3-A10-N2-T3-E2/E12-H7, which is highly divergent from other known non-equine RVA genotype constellations. Phylogenetic analyses revealed that the sequences of these equine RVA strains are related distantly to non-equine RVA strains, and that at least three lineages exist within equine RVA strains. A small number of reassortment events were observed. Interestingly, the three RVA strains from Argentina possessed the E12 genotype, whereas the three RVA strains from Ireland and South Africa possessed the E2 genotype. The unusual E12 genotype has until now only been described in Argentina among RVA strains collected from guanaco, cattle and horses, suggesting geographical isolation of this NSP4 genotype. This conserved genetic configuration of equine RVA strains could be useful for future vaccine development or improvement of currently used equine RVA vaccines.
Publication Date: 2011-12-21 PubMed ID: 22190012DOI: 10.1099/vir.0.039255-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research focuses on sequencing and analyzing the complete molecular genomes of seven equine group A rotavirus strains from different continents, finding several new genotypes and a largely consistent genotype constellation.
Study Overview and Objectives
- The objective of the researchers was to sequence seven strain variants of the equine group A rotavirus (RVA): four from Europe, two from Argentina, and one from South Africa, to analyze their genotype configurations and see how they compare.
- They aimed to identify any new genotypes that might be present, assign them appropriate numbers using the system set up by the Rotavirus Classification Working Group (RCWG), and assess the degree of genetic diversity and similarity between the strains.
Findings and Observations
- The researchers identified and assigned several new genotypes: R9 (VP1), C9 (VP2), N9 (NSP2), T12 (NSP3), E14 (NSP4), and H7 and H11 (NSP5).
- One strain (L338 from Europe) had a unique genotype constellation: G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11.
- The other six exhibited a largely conserved genotype constellation: G3/G14-P[12]-I2/I6-R2-C2-M3-A10-N2-T3-E2/E12-H7. This constellation was significantly different from other known non-equine RVA genotype constellations.
- Their phylogenetic analysis revealed that these horse RVA strain sequences were quite distant from non-equine RVA strains, indicating that at least three separate lineages exist within equine RVA strains.
- Alimited number of reassortment events (a process of genetic recombination in viruses) were observed among the sequences.
- The three Argentina-originated RVA strains had the E12 genotype. Contrastingly, the three strains from Ireland and South Africa had the E2 genotype. Interestingly, the E12 genotype had only previously been seen in strains from Argentina and in guanacos, cattle, and horses, suggesting geographical isolation of this NSP4 genotype.
Implications and Conclusion
- The study enhances our understanding of the genetic makeup and evolution of equine RVA strains. It presents valuable insight into their similarity and divergence, especially in the prevalence of a largely conserved genotype constellation among six of the strains.
- The discovery of several new genotypes, and the realization that certain genotypes (like E12) appear geographically limited, can be vital for vaccination strategies. The genetically conserved strains could possibly be helpful in designing or improving future vaccines.
Cite This Article
APA
Matthijnssens J, Miño S, Papp H, Potgieter C, Novo L, Heylen E, Zeller M, Garaicoechea L, Badaracco A, Lengyel G, Kisfali P, Cullinane A, Collins PJ, Ciarlet M, O'Shea H, Parreño V, Bányai K, Barrandeguy M, Van Ranst M.
(2011).
Complete molecular genome analyses of equine rotavirus A strains from different continents reveal several novel genotypes and a largely conserved genotype constellation.
J Gen Virol, 93(Pt 4), 866-875.
https://doi.org/10.1099/vir.0.039255-0 Publication
Researcher Affiliations
- Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
- Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
- Ondersterpoort Veterinary Institute, Onderstepoort, South Africa.
- Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
- Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
- Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
- Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
- Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
- Dr György Radó Military Medical Centre, Budapest, Hungary.
- Department of Medical Genetics, University of Pécs, Pécs, Hungary.
- Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
- Molecular Epidemiology Laboratory, Cork Institute of Technology, Cork, Ireland.
- Clinical Research and Development, Novartis Vaccines and Diagnostics, Inc., Cambridge, MA 02139, USA.
- Molecular Epidemiology Laboratory, Cork Institute of Technology, Cork, Ireland.
- Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
- Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
- Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
MeSH Terms
- Animals
- Base Sequence
- Conserved Sequence / genetics
- Gastroenteritis / veterinary
- Gastroenteritis / virology
- Genes, Viral / genetics
- Genome, Viral / genetics
- Genotype
- Horse Diseases / virology
- Horses / virology
- Molecular Sequence Data
- Phylogeny
- Rotavirus / genetics
- Rotavirus Infections / veterinary
- Rotavirus Infections / virology
Citations
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