Comprehensive characteristics of microRNA expression profile of equine sarcoids.
Abstract: Equine sarcoids are the most common neoplasms occurring in horses. Despite frequent occurrence, they are still not well described at the molecular level. Thus, in the present study, we performed a comprehensive comparative analysis of sarcoid miRNAome profile to identify aberrantly expressed microRNAs, along with their structural variants, potentially useful as biomarkers and, in a wider perspective, broaden the knowledge about this tumor and underlying mechanisms. To this end, we conducted next generation sequencing and as a result we identified both known and potentially novel miRNAs. Differential expression analysis revealed the existence of almost one hundred miRNAs being over- or underexpressed in sarcoids in comparison to healthy tissue (p-adj<0.05), of which many are known for their involvement in processes crucial for neoplastic transformation. Among upregulated miRNAs there were those associated with decreased cell adhesion abilities as well as engaged in global protein production, while downregulation of some miRs i.a. increased cell expansion abilities. Moreover, we identified altered expression levels of miRNA variants (isomiRs) between the investigated tissues. Further analysis revealed that 5' isomiRs comprise different seed sequences leading to target gene switching followed by activation of different biological pathways. Our results are the first which revealed the complexity of microRNA profiles in equine sarcoids and skin tissue, along with the dynamism of their growing in importance concomitants, namely isomiRs. They also showed miRNA molecules and biological pathways important from the sarcoid oncogenesis point of view.
Copyright © 2017 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.
Publication Date: 2017-03-01 PubMed ID: 28259757DOI: 10.1016/j.biochi.2017.02.017Google Scholar: Lookup
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- Journal Article
Summary
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The research discusses a study on equine sarcoids, a common type of horse neoplasm, to understand its molecular attributes using microRNA expression data. The team also managed to identify new potential biomarkers and presented new insights on sarcoid oncogenesis.
Objective of the Research
- This research aims to deeply understand equine sarcoids, which are common in horses but poorly understood at the molecular level.
- Researchers aimed to uncover any distinctive microRNA expression patterns and structural variants in equine sarcoids, potentially useful as biomarkers.
Methodology and Results
- Next-generation sequencing was used to examine the microRNA expression of equine sarcoids.
- Through the study, known and potentially new microRNAs were identified.
- Almost a hundred overexpressed or underexpressed microRNAs were identified in sarcoids compared to healthy tissue.
- Many of these differentially expressed microRNAs are involved in processes crucial to neoplastic transformation.
- Some upregulated microRNAs were associated with decreased cell adhesion and global protein production, while downregulation of some others increased cell expansion abilities.
- Besides, changes in the expression levels of microRNA variants (isomiRs) were also found between the investigated tissues. These isomiRs affected different biological pathways due to their varying seed sequences, thereby changing their target genes.
Significance of the Research
- This research is a pioneering study in elucidating the complexity of the microRNA expression patterns in equine sarcoids and skin tissue, as well as the role of isomiRs.
- It has shed light on the important microRNA molecules and biological pathways with potential implications in sarcoid oncogenesis.
- The identification of potential biomarkers through the differential expression of certain microRNAs can contribute to better diagnostic tools for equine sarcoids in the future.
Cite This Article
APA
Pawlina K, Gurgul A, Szmatoła T, Koch C, Mählmann K, Witkowski M, Bugno-Poniewierska M.
(2017).
Comprehensive characteristics of microRNA expression profile of equine sarcoids.
Biochimie, 137, 20-28.
https://doi.org/10.1016/j.biochi.2017.02.017 Publication
Researcher Affiliations
- Laboratory of Genomics, Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland. Electronic address: klaudia.pawlina@izoo.krakow.pl.
- Laboratory of Genomics, Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland. Electronic address: artur.gurgul@izoo.krakow.pl.
- Laboratory of Genomics, Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland. Electronic address: tomasz.szmatola@izoo.krakow.pl.
- Swiss Institute of Equine Medicine ISME, Faculty of Veterinary Medicine, University of Bern and Agroscope, Länggassstrasse 124c, Postfach 8466, CH-3001, Bern, Switzerland. Electronic address: christoph.koch@vetsuisse.unibe.ch.
- Swiss Institute of Equine Medicine ISME, Faculty of Veterinary Medicine, University of Bern and Agroscope, Länggassstrasse 124c, Postfach 8466, CH-3001, Bern, Switzerland; Equine Clinic: Surgery and Radiology, Department of Veterinary Medicine, Free University of Berlin, Oertzenweg 19b, 14163, Berlin, Germany. Electronic address: Kathrin.Maehlmann@fu-berlin.de.
- Department of Animal Reproduction, Institute of Veterinary Medicine, University of Agriculture, Al. Mickiewicza 21, 31-120, Krakow, Poland. Electronic address: m.witkowski@ur.krakow.pl.
- Laboratory of Genomics, Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice, Poland. Electronic address: monika.bugno@izoo.krakow.pl.
MeSH Terms
- Animals
- Gene Expression Regulation, Neoplastic
- High-Throughput Nucleotide Sequencing
- Horse Diseases / genetics
- Horse Diseases / metabolism
- Horses
- Humans
- MicroRNAs / genetics
- Skin Neoplasms / genetics
- Skin Neoplasms / metabolism
- Skin Neoplasms / veterinary
- Transcriptome
Citations
This article has been cited 12 times.- Pawlina-Tyszko K, Semik-Gurgul E, Ząbek T, Witkowski M. Methylation Status of Gene Bodies of Selected microRNA Genes Associated with Neoplastic Transformation in Equine Sarcoids. Cells 2022 Jun 14;11(12).
- Yuan J, Jiang L, Guo C. The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma. RSC Adv 2019 Jun 14;9(33):19057-19064.
- Hamza E, Cosandey J, Gerber V, Koch C, Unger L. The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids. Vet Res Commun 2023 Jan;47(1):87-98.
- Cosandey J, Hamza E, Gerber V, Ramseyer A, Leeb T, Jagannathan V, Blaszczyk K, Unger L. Diagnostic and prognostic potential of eight whole blood microRNAs for equine sarcoid disease. PLoS One 2021;16(12):e0261076.
- Pawlina-Tyszko K, Oczkowicz M, Gurgul A, Szmatoła T, Bugno-Poniewierska M. MicroRNA profiling of the pig periaqueductal grey (PAG) region reveals candidates potentially related to sex-dependent differences. Biol Sex Differ 2020 Dec 11;11(1):67.
- Unger L, Abril C, Gerber V, Jagannathan V, Koch C, Hamza E. Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys. J Vet Intern Med 2021 Jan;35(1):610-619.
- Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals (Basel) 2020 May 19;10(5).
- Unger L, Jagannathan V, Pacholewska A, Leeb T, Gerber V. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. J Vet Intern Med 2019 Jan;33(1):241-250.
- Beermann A, Hamza E, Reinhard S, Koch C, Oberhänsli T, Unger L. Selected microRNAs as biomarkers in sarcoid-affected horses under immunotherapy with a mistletoe extract. J Vet Diagn Invest 2026 Jan;38(1):33-40.
- Cullen JN, Cieslak J, Petersen JL, Bellone RR, Finno CJ, Kalbfleisch TS, Calloe K, Capomaccio S, Cappelli K, Coleman SJ, Distl O, Durward-Akhurst SA, Giulotto E, Hamilton NA, Hill EW, Katz LM, Klaerke DA, Lindgren G, MacHugh DE, Mackowski M, MacLeod JN, Metzger J, Murphy BA, Orlando L, Raudsepp T, Silvestrelli M, Strand E, Tozaki T, Trachsel DS, Valderrama Figueroa LS, Velie BD, Wade CM, Waud B, Mickelson JR, McCue ME. Charting the equine miRNA landscape: An integrated pipeline and browser for annotating, quantifying, and visualizing expression. PLoS Genet 2025 Sep;21(9):e1011835.
- Vychodilova L, Plasil M, Futas J, Kopecka A, Molinkova D, Wijacki T, Jahn P, Knoll A, Horin P. Genetic susceptibility to sarcoid in Arabian horses: associations with MHC class II and compound MHC class I/KLRA genotypes. Vet Res Commun 2025 May 1;49(3):184.
- Pawlina-Tyszko K, Szmatoła T. Benchmarking of bioinformatics tools for NGS-based microRNA profiling with RT-qPCR method. Funct Integr Genomics 2023 Nov 30;23(4):347.
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