Computerised analysis of standardised ultrasonographic images to monitor the repair of surgically created core lesions in equine superficial digital flexor tendons following treatment with intratendinous platelet rich plasma or placebo.
Abstract: The effectiveness of new therapies to treat tendon injuries is difficult to determine and is often based on semi-quantitative methods, such as grey level analysis of ultrasonographic images or subjective pain scores. The alternatives are costly and long-lasting end-stage studies using experimental animals. In this study, a method of ultrasonographic tissue characterisation (UTC), using mathematical analysis of contiguous transverse ultrasonographic images, was used for intra-vital monitoring of the healing trajectory of standardised tendon lesions treated with platelet rich plasma (PRP) or placebo. Using UTC it was possible to detect significant differences between the groups in the various phases of repair. At end stage, over 80% of pixels showed correct alignment in the PRP group, compared with just over 60% in the placebo group (P<0.05). UTC also showed significant differences in the course of the healing process between PRP treated and placebo treated animals throughout the experiment. It was concluded that computerised analysis of ultrasonographic images is an excellent tool for objective longitudinal monitoring of the effects of treatments for superficial digital flexor tendon lesions in horses.
2009 Elsevier Ltd. All rights reserved.
Publication Date: 2009-11-20 PubMed ID: 19932036DOI: 10.1016/j.tvjl.2009.10.014Google Scholar: Lookup
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- Journal Article
Summary
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This study examines the effectiveness of platelet rich plasma (PRP) treatment in healing tendon injuries in horses, using ultrasonographic tissue characterisation (UTC) for tracking the healing process. The findings suggest that UTC can successfully identify significant differences in the healing trajectories of tendons treated with PRP versus a placebo.
Objective of the Research
- The study is aimed at assessing the value of computerized analysis of ultrasonographic images as a method for monitoring the repair of tendon injuries in horses. The treatment methods under examination are the intratendinous application of platelet rich plasma (PRP) and a placebo.
Methodology
- The study used ultrasonographic tissue characterisation (UTC), a mathematical analysis of transverse ultrasonographic images, to monitor the live healing trajectory of standardized tendon injuries in horses that were treated with either PRP or placebo.
- This technique is comparatively less costly and doesn’t require a long-term engagement like other end-stage studies that use experimental animals.
Results
- In the course of the study, UTC was able to detect significant differences in the healing process between tendons treated with PRP and those treated with a placebo at various stages of repair.
- By the end of the study, over 80% of the scans in the PRP treatment group showed correctly aligned pixels, as compared to just over 60% in the placebo group. These results were statistically significant (P<0.05), indicating that the differences are likely not due to chance alone.
Conclusions
- This research concludes that computerized analysis of ultrasonographic images is an efficient method for longitudinally monitoring the effects of treatments for superficial digital flexor tendon lesions in horses.
- It also gives an indication that PRP could potentially be a more effective treatment for tendon injuries in horses than a placebo, which is important for veterinarians and horse owners to know when deciding on treatment options.
Cite This Article
APA
Bosch G, René van Weeren P, Barneveld A, van Schie HT.
(2009).
Computerised analysis of standardised ultrasonographic images to monitor the repair of surgically created core lesions in equine superficial digital flexor tendons following treatment with intratendinous platelet rich plasma or placebo.
Vet J, 187(1), 92-98.
https://doi.org/10.1016/j.tvjl.2009.10.014 Publication
Researcher Affiliations
- Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. g.bosch@uu.nl
MeSH Terms
- Animals
- Female
- Horse Diseases / diagnostic imaging
- Horse Diseases / therapy
- Horses / injuries
- Male
- Platelet-Rich Plasma
- Tendon Injuries / diagnostic imaging
- Tendon Injuries / therapy
- Tendon Injuries / veterinary
- Tendons / diagnostic imaging
- Tendons / pathology
- Ultrasonography
- Wound Healing / physiology
Citations
This article has been cited 16 times.- Chalidis B, Givissis P, Papadopoulos P, Pitsilos C. Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review.. Int J Mol Sci 2023 Feb 1;24(3).
- Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade.. Front Vet Sci 2021;8:641818.
- Montano C, Auletta L, Greco A, Costanza D, Coluccia P, Del Prete C, Meomartino L, Pasolini MP. The Use of Platelet-Rich Plasma for Treatment of Tenodesmic Lesions in Horses: A Systematic Review and Meta-Analysis of Clinical and Experimental Data.. Animals (Basel) 2021 Mar 12;11(3).
- Depuydt E, Broeckx SY, Van Hecke L, Chiers K, Van Brantegem L, van Schie H, Beerts C, Spaas JH, Pille F, Martens A. The Evaluation of Equine Allogeneic Tenogenic Primed Mesenchymal Stem Cells in a Surgically Induced Superficial Digital Flexor Tendon Lesion Model.. Front Vet Sci 2021;8:641441.
- Lawson A, Noorkoiv M, Masci L, Mohagheghi AA. Ankle Joint Position and the Reliability of Ultrasound Tissue Characterization of the Achilles Tendon: A Pilot Study.. Med Sci Monit 2019 Sep 13;25:6884-6893.
- Beerts C, Suls M, Broeckx SY, Seys B, Vandenberghe A, Declercq J, Duchateau L, Vidal MA, Spaas JH. Tenogenically Induced Allogeneic Peripheral Blood Mesenchymal Stem Cells in Allogeneic Platelet-Rich Plasma: 2-Year Follow-up after Tendon or Ligament Treatment in Horses.. Front Vet Sci 2017;4:158.
- Watts AE, Millar NL, Platt J, Kitson SM, Akbar M, Rech R, Griffin J, Pool R, Hughes T, McInnes IB, Gilchrist DS. MicroRNA29a Treatment Improves Early Tendon Injury.. Mol Ther 2017 Oct 4;25(10):2415-2426.
- Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial.. Stem Cell Res Ther 2017 Jun 5;8(1):129.
- White NA 2nd, Barrett JG. Magnetic Resonance Imaging-Guided Treatment of Equine Distal Interphalangeal Joint Collateral Ligaments: 2009-2014.. Front Vet Sci 2016;3:73.
- Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial.. BMC Vet Res 2016 Sep 7;12(1):191.
- González JC, López C, Álvarez ME, Pérez JE, Carmona JU. Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model.. Sci Rep 2016 Jan 19;6:19623.
- Wong AM, Docking SI, Cook JL, Gaida JE. Does type 1 diabetes mellitus affect Achilles tendon response to a 10 km run? A case control study.. BMC Musculoskelet Disord 2015 Nov 10;16:345.
- Salamanna F, Veronesi F, Maglio M, Della Bella E, Sartori M, Fini M. New and emerging strategies in platelet-rich plasma application in musculoskeletal regenerative procedures: general overview on still open questions and outlook.. Biomed Res Int 2015;2015:846045.
- Kuffler DP. Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction.. Mol Neurobiol 2015 Oct;52(2):990-1014.
- Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions.. BMC Vet Res 2015 Apr 22;11:98.
- Silva RF, Alvarez ME, Ríos DL, López C, Carmona JU, Rezende CM. Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates.. BMC Vet Res 2012 Nov 6;8:212.
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