Concentration of amikacin sulphate in synovial fluid when given in combination with dexamethasone phosphate in intravenous regional limb perfusion in standing horses.
Abstract: Eight horses underwent IVRLP at two occasions through a 23-gauge 2 cm long butterfly catheter. Regional anaesthesia of the ulnar, median and medial cutaneous antebrachial nerves was performed prior, and an 8 cm rubber tourniquet was placed on the proximal radius for 30 minutes following the infusion. The first infusion consisted of 2 g of amikacin sulphate and 10 mg of dexamethasone phosphate diluted with 0.9% NaCl to a total volume of 100 ml. The second perfusion was performed after a 2-week washout period, the same protocol was used but without dexamethasone phosphate. Synovial fluid samples were collected from the metacarpophalangeal joint at T = 0, 0.5, 2, 12, 24 and 36 h post-infusion. Synovial fluid amikacin sulphate concentrations were determined by use of liquid chromatography/tandem mass-spectrometry. All horses (n = 8) remained healthy throughout the study, and no adverse effects associated with the study were encountered. No statistically significant differences were found in synovial fluid amikacin sulphate concentrations between the treatment and the control group at any of the time points. In conclusion, dexamethasone phosphate can be used in IVRLP concomitantly with amikacin sulphate in cases of distal limb inflammation and pain without decreasing the synovial fluid concentration of amikacin sulphate.
© 2022 John Wiley & Sons Ltd.
Publication Date: 2022-03-20 PubMed ID: 35307838DOI: 10.1111/jvp.13053Google Scholar: Lookup
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- Journal Article
- Antibiotics
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Dexamethasone
- Disease Treatment
- Drug
- Equine Health
- Horses
- In Vivo
- Inflammation
- Intravenous Administration
- Metacarpophalangeal Joint
- Pain Management
- Pharmacodynamics
- Pharmacokinetics
- Synovial Fluid
- Veterinary Medicine
- Veterinary Practice
- Veterinary Procedure
Summary
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The research was conducted to determine if the joint fluid (synovial fluid) concentrations of Amikacin Sulfate, an antibiotic, would be affected when given in conjunction with Dexamethasone Phosphate, a steroidal anti-inflammatory drug, through a procedure known as intravenous regional limb perfusion (IVRLP) in horses. The results indicated no significant reduction in the concentration of Amikacin Sulfate in the synovial fluid when used with Dexamethasone Phosphate.
Experiment Design and Methodology
- The experiment involved eight horses that went through an IVRLP procedure twice.
- Each IVRLP was facilitated using a 23-gauge 2 cm long butterfly catheter.
- Before every IVRLP procedure, the horses were anaesthetised in the ulnar, median and medial cutaneous antebrachial nerves.
- An 8 cm rubber tourniquet was placed on the proximal radius for 30 minutes following the infusion, in order to restrict the flow of the solutions in the body.
- For the first infusion, a mixture of 2 g of amikacin sulphate and 10 mg of dexamethasone phosphate was used. The mixture was diluted using 0.9% NaCl to a total volume of 100 ml.
- After a 2-week washout period, the same procedure was performed for a second time, this time without including dexamethasone phosphate.
Sample Collection and Analysis
- Synovial fluid samples were collected from the horses’ metacarpophalangeal joint at six time points: immediately after the infusion (T=0), and at 0.5 hrs., 2 hrs., 12 hrs., 24 hrs., and 36 hrs. post-infusion.
- The samples were then subjected to liquid chromatography/tandem mass-spectrometry to determine the concentration of amikacin sulphate in the synovial fluid.
- No adverse effects were observed in any of the horses throughout the study, indicating the procedures were safe.
Research Findings
- The analysis revealed that there was no statistically significant difference in the concentrations of amikacin sulphate in the synovial fluid between the first group (that received both amikacin sulphate and dexamethasone phosphate) and the second group (that received only amikacin).
- These results led to the conclusion that dexamethasone phosphate can be used together with amikacin sulphate in cases of distal limb inflammation and pain in horses, without affecting the concentration of amikacin sulphate in the synovial fluid.
Cite This Article
APA
Gustafsson K, Tatz AJ, Britzi M, Dahan R, Sutton GA, Kelmer G.
(2022).
Concentration of amikacin sulphate in synovial fluid when given in combination with dexamethasone phosphate in intravenous regional limb perfusion in standing horses.
J Vet Pharmacol Ther, 45(4), 409-414.
https://doi.org/10.1111/jvp.13053 Publication
Researcher Affiliations
- Department of Large Animal Medicine and Surgery, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal Medicine and Surgery, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- National Residue Control Laboratory, Kimron Veterinary Institute, Beit Dagan, Israel.
- Department of Large Animal Medicine and Surgery, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal Medicine and Surgery, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
- Department of Large Animal Medicine and Surgery, Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
MeSH Terms
- Amikacin / analysis
- Amikacin / chemistry
- Animals
- Anti-Bacterial Agents / analysis
- Dexamethasone / analogs & derivatives
- Forelimb
- Horses
- Perfusion / veterinary
- Synovial Fluid / chemistry
Grant Funding
- Internal hospital grant
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