Concentrations of serum amyloid A and lipopolysaccharide-binding protein in horses with colic.
Abstract: To determine concentrations of 2 acute-phase proteins (serum amyloid A [SAA] and lipopolysaccharide-binding protein [LBP]) in serum samples obtained from horses with colic and identify relationships among these acute-phase proteins and clinical data. Methods: 765 horses with naturally developing gastrointestinal tract diseases characterized by colic (ie, clinical signs indicative of abdominal pain) and 79 healthy control horses; all horses were examined at 2 university teaching hospitals. Methods: Serum concentrations of SAA and LBP were determined by immunoturbidometric and dot-blot assays, respectively. Results: SAA and LBP concentrations were determined for 718 and 765 horses with colic, respectively. Concentrations of SAA were significantly higher in nonsurvivors than in survivors, and horses with enteritis or colitis and conditions characterized by chronic inflammation (eg, abdominal abscesses, peritonitis, or rectal tears) had SAA concentrations significantly greater than those for horses with other conditions. Serum concentrations of LBP did not correlate with outcome, disease process, or portion of the gastrointestinal tract affected. Conclusions: Circulating concentrations of SAA were significantly higher at admission in horses with colic attributable to conditions having a primary inflammatory cause (eg, enteritis, colitis, peritonitis, or abdominal abscesses) and were higher in horses that failed to survive the episode of colic, compared with concentrations in horses that survived. Serum concentrations of LBP did not correlate with survival. Analysis of these findings suggests that evaluation of SAA concentrations may be of use in identifying horses with colic attributable to diseases that have inflammation as a primary component of pathogenesis.
Publication Date: 2005-11-03 PubMed ID: 16261823DOI: 10.2460/ajvr.2005.66.1509Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Acute Phase Proteins
- Biomarkers
- Clinical Pathology
- Clinical Study
- Colic
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Equine Health
- Gastrointestinal Diseases
- Horses
- Immune Response
- Inflammation
- Inflammatory Response
- Lipopolysaccharide
- Pathogenesis
- Serum
- Serum Amyloid A
- Survival Rate
- Veterinary Medicine
- Veterinary Research
Summary
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This study conducted research on horses affected by colic and the connections between two types of acute-phase proteins (serum amyloid A [SAA] and lipopolysaccharide-binding protein [LBP]) and clinical data from the horses. The results suggested that higher concentrations of SAA at admission were present in horses with inflammation-causing conditions and those that did not survive, whereas LBP levels showed no correlation with survival or disease process.
Study Methods
- The study included a total of 844 horses. Among these, 765 were affected by naturally occurring gastrointestinal diseases, characterized by colic, while 79 healthy control horses were also included for comparison.
- Examinations of the horses were conducted at two university teaching hospitals.
- The researchers used immunoturbidometric and dot-blot assays, respectively, to measure the SAA and LBP serum concentrations in the horses.
Key Findings
- The SAA concentrations were established in 718 horses and LBP concentrations in 765 horses suffering from colic.
- The data displayed significantly higher levels of SAA concentration in horses that did not survive the instance of colic than those who did.
- Also, horses suffering from enteritis, colitis, and other conditions caused by chronic inflammation (e.g., abdominal abscesses, peritonitis, rectal tears) showed significantly higher SAA concentrations than horses with different diseases.
- Unlike SAA, levels of LBP did not show any correlation with the survival rate, the disease process, or the affected portion of the gastrointestinal tract.
Implication of the Findings
- The findings highlight a significant observation of higher SAA levels upon admission in horses suffering from colic due to primary inflammatory conditions like enteritis, colitis, peritonitis, or abdominal abscesses.
- Further, higher levels of SAA have been linked with instances where the horses failed to survive the episode of colic.
- On the other hand, LBP serum concentration did not show any correlation with the survival outcomes, pointing out that this protein may not be useful for determining the prognosis of horses suffering from colic.
- The patterns discovered in this study propose that evaluating SAA concentrations can potentially help to identify horses with colic stemming from diseases with inflammation as a primary aspect of the disease process.
Cite This Article
APA
Vandenplas ML, Moore JN, Barton MH, Roussel AJ, Cohen ND.
(2005).
Concentrations of serum amyloid A and lipopolysaccharide-binding protein in horses with colic.
Am J Vet Res, 66(9), 1509-1516.
https://doi.org/10.2460/ajvr.2005.66.1509 Publication
Researcher Affiliations
- Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
MeSH Terms
- Acute-Phase Proteins
- Animals
- Carrier Proteins / blood
- Colic / blood
- Colic / veterinary
- Horse Diseases / blood
- Horses
- Immunoblotting / veterinary
- Membrane Glycoproteins / blood
- Nephelometry and Turbidimetry / veterinary
- Serum Amyloid A Protein / metabolism
- Statistics, Nonparametric
Citations
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